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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2251 [new locus tag: NWMN_RS12960 ]
  • pan locus tag?: SAUPAN005849000
  • symbol: NWMN_2251
  • pan gene symbol?: —
  • synonym:
  • product: esterase-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2251 [new locus tag: NWMN_RS12960 ]
  • symbol: NWMN_2251
  • product: esterase-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2472675..2473595
  • length: 921
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    TTGATTAGAAACCGTGTTATGAATAGCGTTGTCAATAAATATTTGCTTCACAATCGCTCG
    ATTATGTTTAAAAATGATCAAGATGTTGAAAGATTTTTTTATAAAAGAGAAATTGAAAAT
    AGAAAAAAACATAAGCAGCCTTCAACATTAAATGTTAAAGCAAATTTAGAAAAATTGTCA
    TTAGATGATATGCAAGTCTTTCGCTTTAATTTCAGACATCAAATTGATAAGAAAATTTTA
    TATATTCACGGTGGATTCAATGCACTACAACCATCACCGTTCCATTGGAGATTGTTGGAT
    AAAATCACTTTAAGTACATTATATGAGGTTGTACTGCCTATCTATCCAAAGACACCAGAG
    TTTCATATTGACGATACTTTCCAAGCGATACAACGTGTTTATGATCAATTAGTATCTGAA
    GTAGGACATCAAAATGTCGTAGTCATGGGTGATGGTTCAGGTGGTGCACTGGCATTATCC
    TTTGTACAATCTCTTTTAGATAATCAACAGCCATTACCGAATAAATTGTATTTAATCTCA
    CCAATTTTAGATGCAACACTATCTAATAAAGATATTTCGGACGCCTTAATCGAACAAGAT
    GCGGTTCTAAGTCAGTTTGGTGTCAATGAGATTATGAAAAAATGGGCGAATGGCCTACCA
    TTAACAGATAAGCGCATATCGCCAATTAATGGCACAATAGAAGGATTGCCACCAGTTTAT
    ATGTTTGGTGGTGGACGTGAAATGACACATCCTGATATGAAGCTATTTGAACAAATGATG
    TTGCAACATCATCAATACATTGAGTTTTATGATTATCCTAAGATGGTACATGATTTTCCA
    ATTTATCCAATTAGACAATCACATAAAGCTATTAAACAAATTGCCAAATCGATAGATGAG
    GATGTAACACAAAATAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2251 [new locus tag: NWMN_RS12960 ]
  • symbol: NWMN_2251
  • description: esterase-like protein
  • length: 306
  • theoretical pI: 8.02735
  • theoretical MW: 35544.7
  • GRAVY: -0.389216

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 97.9)
    and 3 more
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 55.6)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 14.4)
    DUF3530; Protein of unknown function (DUF3530) (PF12048; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002179
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000297
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKHKQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKITLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALSFVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANGLPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMVHDFPIYPIRQSHKAIKQIAKSIDEDVTQNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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