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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2295 [new locus tag: NWMN_RS13200 ]
  • pan locus tag?: SAUPAN005926000
  • symbol: narJ
  • pan gene symbol?: narJ
  • synonym:
  • product: respiratory nitrate reductase, delta subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2295 [new locus tag: NWMN_RS13200 ]
  • symbol: narJ
  • product: respiratory nitrate reductase, delta subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2519606..2520196
  • length: 591
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAGGGATTTTCCGTGATTAATTTCGATAATTTTAAAAAATATCAAGAAAGTTTTGGA
    TATATGGCACAACAGTTATGTTTTCCAGAAAAATTAACTTTTCATCCAAAAACTTTTGAA
    GAAACAATTTCGAAAGATCATCCAGCATATGAAGATTTAGTAGCATTTAGAAATGTAATG
    ATGACATTTTCATTATCAGAAATCAAAGCGATTTATACAGATACATTTGATTTTACGAAA
    AAAGCACCATTATATATGACGTATAACAAATTTGATACGCAAAAAGAACGTGGGCAAATG
    TTAGCTAAATTAAAGGTATTATATGAAATGTTTGGACTAGAAATGGTAGATAATGAACTT
    TCTGATTATCTACCGTTGATGCTTCAATTTTTGCAAGTGGCAGAATGGCGAAATGATCCA
    AGAGCTGAAGAGAATATTCAATTAATCATTATGATTATTGAGGATGGAACATATGTGATG
    GCGAATGCACTTGGTGAAGATAACAATCCATATGCCTATGTGATTCAAGCACTTAGAAAA
    ACATTGAAATTATGTTTGACATCACCAAAAGGAGTGAAACATCATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    591

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2295 [new locus tag: NWMN_RS13200 ]
  • symbol: NarJ
  • description: respiratory nitrate reductase, delta subunit
  • length: 196
  • theoretical pI: 5.09662
  • theoretical MW: 22925.3
  • GRAVY: -0.286735

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization nitrate reductase molybdenum cofactor assembly chaperone (TIGR00684; HMM-score: 65.6)
    and 1 more
    DMSO reductase family type II enzyme chaperone (TIGR03482; HMM-score: 26.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Nitrate_red_del; Nitrate reductase delta subunit (PF02613; HMM-score: 25.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003696
    • TAT(Tat/SPI): 0.000164
    • LIPO(Sec/SPII): 0.000675
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEGFSVINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPAYEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVLYEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALGEDNNPYAYVIQALRKTLKLCLTSPKGVKHHA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NreC* (activation) regulon
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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