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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2308 [new locus tag: NWMN_RS13275 ]
  • pan locus tag?: SAUPAN005943000
  • symbol: dsbA
  • pan gene symbol?: dsbA
  • synonym:
  • product: protein-disulfide isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2308 [new locus tag: NWMN_RS13275 ]
  • symbol: dsbA
  • product: protein-disulfide isomerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2535803..2536402
  • length: 600
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACTAAAAAATTACTAACATTATTTATAGTGAGCATGTTAATTTTAACAGCTTGCGGT
    AAAAAAGAATCAGCAACGACATCTTCGAAAAACGGCAAACCATTAGTTGTCGTATATGGC
    GACTATAAATGTCCTTATTGTAAAGAATTAGATGAAAAAGTCATGCCAAAGTTGCGTAAA
    AATTATATAGATAATCACAAAGTGGAATACCAATTTGTCAATTTAGCTTTCTTAGGTAAA
    GACTCAATTGTTGGTTCGCGTGCGAGTCATGCAGTATTGATGTATGCACCTAAATCATTT
    TTAGATTTTCAAAAGCAATTATTTGCTGCCCAGCAAGATGAAAATAAAGAATGGTTAACA
    AAAGAACTATTAGATAAACATATTAAACAACTGCATTTAGATAAAGAGACGGAAAATAAA
    ATTATAAAAGATTACAAGACAAAAGATAGCAAGTCTTGGAAAGCTGCAGAGAAAGATAAA
    AAAATAGCGAAAGATAATCATATAAAAACGACACCAACTGCATTTATTAATGGCGAGAAA
    GTTGAAGATCCATATGATTATGAAAGTTATGAGAAGTTATTAAAAGATAAAATCAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2308 [new locus tag: NWMN_RS13275 ]
  • symbol: DsbA
  • description: protein-disulfide isomerase
  • length: 199
  • theoretical pI: 9.67715
  • theoretical MW: 23061.6
  • GRAVY: -0.680402

⊟Function[edit | edit source]

  • TIGRFAM:
    glutaredoxin, GrxA family (TIGR02183; HMM-score: 16.9)
    glutaredoxin (TIGR02180; HMM-score: 15.9)
    Metabolism Energy metabolism Electron transport glutaredoxin 3 (TIGR02181; HMM-score: 13.6)
    and 2 more
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 11.4)
    extracellular lipase, Pla-1/cef family (TIGR03502; EC 3.1.-.-; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Thioredoxin (CL0172) Thioredoxin_4; Thioredoxin (PF13462; HMM-score: 96.1)
    and 10 more
    Thioredoxin_2; Thioredoxin-like domain (PF13098; HMM-score: 33.3)
    DSBA; DSBA-like thioredoxin domain (PF01323; HMM-score: 32.7)
    Thioredoxin_5; Thioredoxin (PF13743; HMM-score: 18.6)
    no clan defined Imm42; Immunity protein 42 (PF15593; HMM-score: 17.6)
    Thioredoxin (CL0172) Glutaredoxin; Glutaredoxin (PF00462; HMM-score: 16.8)
    Thioredoxin_7; Thioredoxin-like (PF13899; HMM-score: 16.8)
    Thioredoxin_3; Thioredoxin domain (PF13192; HMM-score: 15.1)
    C2H2-zf (CL0361) zf-Di19; Drought induced 19 protein (Di19), zinc-binding (PF05605; HMM-score: 14.1)
    Zn_Beta_Ribbon (CL0167) Toprim_Crpt; C-terminal repeat of topoisomerase (PF13342; HMM-score: 13)
    Thioredoxin (CL0172) Thioredoxin_8; Thioredoxin-like (PF13905; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: ILTACGK
  • SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
    • SP(Sec/SPI): 0.001408
    • TAT(Tat/SPI): 0.000083
    • LIPO(Sec/SPII): 0.998331
    • Cleavage Site: CS pos: 18-19. LTA-CG. Pr: 0.9990
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKCPYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLDFQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDKKIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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