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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2471 [new locus tag: NWMN_RS14195 ]
- pan locus tag?: SAUPAN006240000
- symbol: NWMN_2471
- pan gene symbol?: —
- synonym:
- product: TetR family regulatory protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2471 [new locus tag: NWMN_RS14195 ]
- symbol: NWMN_2471
- product: TetR family regulatory protein
- replicon: chromosome
- strand: +
- coordinates: 2720923..2721510
- length: 588
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331529 NCBI
- RefSeq: YP_001333505 NCBI
- BioCyc:
- MicrobesOnline: 3708073 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGTTAGGTAGTGAACAAATGATGGAAAGTAAATCCATAGATCCAAGAATCGTTAGAACC
AAACAATTGCTTGTCGATGCTTTTCTTAAAATTTCTAGAGAAAAGAAATTATCTCAAATT
ACAGTTAAAGATATCACTGATATTGCTACGCTAAATCGTGCAACATTTTACGCTCATTTC
ACTGATAAAGAAGACCTCCTAGACTACACATTATCTGTAACCATTTTAAAAGACTTGAAT
GATAATTTGAGCATTTCTAATGTCATTAATGAAAAGGTTCTGCGTAATATTTTCATTTCA
ATTGCGAGTTATATCAAAGATGCTGCAAAGTCTTGCGAATTAAATAGTGAAGCATTTTGC
AACAAAGCACATCAACGTATTAATAATGAATTAGAAGATATTTTTGCGATTATGTTAGAA
AACAGCTATCCGGAGCATCAACGAGATATCATTGTAAATAGTGCGAGTTTTTTAGCAGCT
GGTATCTCAGGCTTAGCATTACATTGGTTTAACACGAGTCAAGAGACAGCCGATGTGTTT
ATCGATCGCAACCTTCCATTTTTAATTCATCATATAGCACATTTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2471 [new locus tag: NWMN_RS14195 ]
- symbol: NWMN_2471
- description: TetR family regulatory protein
- length: 195
- theoretical pI: 5.8754
- theoretical MW: 22231.3
- GRAVY: -0.0733333
⊟Function[edit | edit source]
- TIGRFAM: probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 40.3)and 2 moreRegulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 20.4)Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 16.4)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 27.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001384
- TAT(Tat/SPI): 0.000183
- LIPO(Sec/SPII): 0.000284
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLGSEQMMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIATLNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDAAKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLALHWFNTSQETADVFIDRNLPFLIHHIAHF
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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