PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2475 [new locus tag: NWMN_RS14215 ]
• pan locus tag^?: SAUPAN006249000
• symbol: NWMN_2475
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2475 [new locus tag: NWMN_RS14215 ]
• symbol: NWMN_2475
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2723202..2724023
• length: 822
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331533 NCBI
• RefSeq: YP_001333509 NCBI
• BioCyc:
• MicrobesOnline: 3708077 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGATATTTTAGTAATAGGCGCAACAGGGAAGCAAGGTAATGCAGTTGTAAAACAG
  TTGTTAGAGGATGGATGGTATGTAAGTGCATTAACTAGAAATAAGAATAATCGTAAACTT
  TCAGACATAGGACATCCGCATTTATCAATCGTTGAAGGTGATCTGAGTGATAATGTTAGT
  TTGCAATCAGCGATGAAAGGTAAGTATGGCTTATACAGTATTCAGCCAATCGTTAAAGAT
  GATGTTAGCGAAGAATTAAGACAAGGTATGAAGATAATTGAAATAGCTGAGCAAGAAAAT
  ATTCAACATATTGTGTATAGTACTGCGGGAGGGGTTAATCGAAATCGAACTGGCCCACAC
  TTTGAAGTACTAGCAAAAATAGAAAATAGATTAATGGAATCTAATATAAATGCAACAGTT
  ATCAAACCATCATTCTTTATGGATAATTTTTTACGCATTGCTAAAGTCGAAGATGAACGT
  ATTACATTACCAGAATTTATTAATCCGAATATAAAATTCACAATGATTTCTTCTATTGAT
  ATAGCTAAAATTGCATCGTATATTTTTGCACATCCACAAAGCTTTACGCATCAATCAATA
  GAGATTGGTTCCGATGAAGTTACATTAAGTGAAGCAGCAACAATTTTTAGTGAAGTGACA
  GGTAAATCTACTGTTATAGAAGGAGAATTTGTTAGTGGTGTTGCAGAAAAGCAATGGTTG
  GAAGAAAAAGGTTATGAAGTAGACTTTGAATTAATGGCTGAAATAAATCCAACAAGATTA
  TCATTAAGTGATTGGCTTAAAGTCCAAAACTATAATAAGTAA
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  822
  

⊟Protein

⊟General

• locus tag: NWMN_2475 [new locus tag: NWMN_RS14215 ]
• symbol: NWMN_2475
• description: hypothetical protein
• length: 273
• theoretical pI: 4.95399
• theoretical MW: 30608.6
• GRAVY: -0.237363

⊟Function

• TIGRFAM:
  ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 42.7)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 41.9)
  and 4 more
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 16.4)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 14.3)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 13.7)
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 11.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) NmrA; NmrA-like family (PF05368; HMM-score: 135.7)
  and 9 more
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 76.5)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 30.5)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 17.7)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 17.3)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 16.2)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.5)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 12.9)
  DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 12.6)
  HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007367
  • TAT(Tat/SPI): 0.000196
  • LIPO(Sec/SPII): 0.00085
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222687 NCBI
• RefSeq: YP_001333509 NCBI
• UniProt: A0A0H3KAP5 UniProt

⊟Protein sequence

• MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIGHPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQHIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDERITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSEVTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2474 < NWMN_2475 < NWMN_2476

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications