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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2526 [new locus tag: NWMN_RS14520 ]
  • pan locus tag?: SAUPAN006349000
  • symbol: phoB
  • pan gene symbol?: phoB
  • synonym:
  • product: alkaline phosphatase III precursor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2526 [new locus tag: NWMN_RS14520 ]
  • symbol: phoB
  • product: alkaline phosphatase III precursor
  • replicon: chromosome
  • strand: +
  • coordinates: 2777169..2778593
  • length: 1425
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACG
    GTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGT
    TTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATG
    GGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATG
    ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCA
    AAACAGAATATTACTGATTCTGCAGCTGGCGGAACAGCCTTTAGTTCTGGTCATAAGACA
    TATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAGTAAAGACGGTGCTTGAACGT
    GCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
    CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAA
    TTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCT
    AAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAC
    CTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
    GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGAT
    ATGCAACAAAGTGCACTTAGTAAGCTTGAGCGCAATAAAAAAGGATTCTTTTTAATGGTC
    GAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATATTACAGGCGTTATGTCTGAA
    ATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
    ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAA
    GGATACGAATGGAATCCTCAACCGATTAAATCTATGAAACACTCTGGATCATATATGACA
    GAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTAATGAAGGTTATGGATTTGAA
    TTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGCTAAAAAGACTACTT
    AAAAAAGAAAAAGACGAAAAATCCGCAGAAGTAGAAACGCAAACAAAAGCCCTACAACAC
    GCTATTCAGAAGCCAGTAAATGATAAATCTTATACAGGGTGGACTTCCGATGGACATACA
    GGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAGAATCATTTAGTGGTAATATC
    GAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1425

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2526 [new locus tag: NWMN_RS14520 ]
  • symbol: PhoB
  • description: alkaline phosphatase III precursor
  • length: 474
  • theoretical pI: 9.60182
  • theoretical MW: 51960
  • GRAVY: -0.759072

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Alk_phosphatase (CL0088) Alk_phosphatase; Alkaline phosphatase (PF00245; HMM-score: 388.4)
    and 4 more
    Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 24.6)
    no clan defined Pox_H7; Late protein H7 (PF04787; HMM-score: 15.9)
    DUF1889; Domain of unknown function (DUF1889) (PF08986; HMM-score: 14.7)
    TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TPAIAQSD
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.991774
    • TAT(Tat/SPI): 0.003916
    • LIPO(Sec/SPII): 0.002577
    • Cleavage Site: CS pos: 30-31. AIA-QS. Pr: 0.8454
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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