PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2420 [new locus tag: SA_RS13880 ]
• pan locus tag^?: SAUPAN006349000
• symbol: phoB
• pan gene symbol^?: phoB
• synonym:
• product: alkaline phosphatase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2420 [new locus tag: SA_RS13880 ]
• symbol: phoB
• product: alkaline phosphatase
• replicon: chromosome
• strand: +
• coordinates: 2715011..2716435
• length: 1425
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125349 NCBI
• RefSeq: NP_375746 NCBI
• BioCyc: see SA_RS13880
• MicrobesOnline: 104772 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACA
  GTTTTCAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGT
  TTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATG
  GGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATTCGCATGCCAAAGAAATG
  ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCA
  AAACAGAATATTACTGATTCTGCAGCTGGCGGAACAGCCTTTAGTTCTGGCCATAAGACA
  TATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAGTAAAGACAGTGCTTGAACGT
  GCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
  CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAA
  TTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCT
  AAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAC
  CTTGCCACGAACAGTAAGGATTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
  GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGAT
  ATGCAACAAAGTGCACTTAGTAAGCTTGAGCGTAATAAAAAAGGTTTCTTTTTAATGGTC
  GAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATATTACAGGCGTTATGTCTGAA
  ATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
  ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAA
  GGATACGAATGGAATCCTCAACCGATTAAATCGATGAAACACTCTGGATCATATATGACA
  GAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTAATGAAGGTTATGGCTTTGAA
  TTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGTTGCAAAAATTACTT
  AAAAAAGAAAAAGACGAAAAATCTGCAGATGTAGAAAAACAAACAAAAGCCTTACAACAC
  GCAATTCAGAAGCCAGTAAATGATAAATCTTATACAGGGTGGACTTCCGATGGACATACA
  GGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAGAATCATTTAGTGGTAATATC
  GAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA
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⊟Protein

⊟General

• locus tag: SA2420 [new locus tag: SA_RS13880 ]
• symbol: PhoB
• description: alkaline phosphatase
• length: 474
• theoretical pI: 9.5982
• theoretical MW: 51920.9
• GRAVY: -0.762025

⊟Function

• reaction:
  EC 3.1.3.1?  ExPASy
  Alkaline phosphatase A phosphate monoester + H₂O = an alcohol + phosphate
• TIGRFAM:
• TheSEED  :
  • Alkaline phosphatase (EC 3.1.3.1)
  Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Alkaline phosphatase (EC 3.1.3.1)
• PFAM:
  Alk_phosphatase (CL0088) Alk_phosphatase; Alkaline phosphatase (PF00245; HMM-score: 389)
  and 4 more
  Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 24.9)
  no clan defined DUF1889; Domain of unknown function (DUF1889) (PF08986; HMM-score: 14.8)
  TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 14.1)
  NADP_Rossmann (CL0063) Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 14.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: TPAIAQSD
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.991774
  • TAT(Tat/SPI): 0.003916
  • LIPO(Sec/SPII): 0.002577
  • Cleavage Site: CS pos: 30-31. AIA-QS. Pr: 0.8454
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928213 NCBI
• RefSeq: NP_375746 NCBI
• UniProt: A0A0H3JWA7 UniProt

⊟Protein sequence

• MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications