PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2576 [new locus tag: NWMN_RS14780 ]
• pan locus tag^?: SAUPAN006433000
• symbol: hisD
• pan gene symbol^?: hisD
• synonym:
• product: histidinol dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2576 [new locus tag: NWMN_RS14780 ]
• symbol: hisD
• product: histidinol dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2843940..2845190
• length: 1251
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332001 NCBI
• RefSeq: YP_001333610 NCBI
• BioCyc:
• MicrobesOnline: 3708178 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
  TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
  AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
  CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
  TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
  TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTGCCTGGT
  GGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTAGCAGGT
  GTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAAGTATTA
  GCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAAAGTATT
  GCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGC
  AATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGACCAAATA
  GCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCCATCGTA
  TATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATTAGTGAA
  GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
  AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
  TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
  GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
  TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
  AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
  ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
  GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
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⊟Protein

⊟General

• locus tag: NWMN_2576 [new locus tag: NWMN_RS14780 ]
• symbol: HisD
• description: histidinol dehydrogenase
• length: 416
• theoretical pI: 4.80567
• theoretical MW: 46177.7
• GRAVY: -0.176683

⊟Function

• reaction:
  EC 1.1.1.23?  ExPASy
  Histidinol dehydrogenase L-histidinol + H₂O + 2 NAD⁺ = L-histidine + 2 NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 474)
  and 8 more
  no clan defined HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
  DUF4435; Protein of unknown function (DUF4435) (PF14491; HMM-score: 14.2)
  MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
  ZapB; Cell division protein ZapB (PF06005; HMM-score: 13.3)
  YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 13.1)
  no clan defined FadA; Adhesion protein FadA (PF09403; HMM-score: 12)
  YtxH; YtxH-like protein (PF12732; HMM-score: 10.5)
  DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001818
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000226
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222788 NCBI
• RefSeq: YP_001333610 NCBI
• UniProt: A0A0H3KAR9 UniProt

⊟Protein sequence

• MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications