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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman
locus tag: NWMN_RS14780 [old locus tag: NWMN_2576 ]
pan locus tag^?: SAUPAN006433000
symbol: NWMN_RS14780
pan gene symbol^?: hisD
synonym:
product: histidinol dehydrogenase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_RS14780 [old locus tag: NWMN_2576 ]
symbol: NWMN_RS14780
product: histidinol dehydrogenase
replicon: chromosome
strand: -
coordinates: 2843940..2845190
length: 1251
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_009641 (2843940..2845190) NCBI
BioCyc:
MicrobesOnline: see NWMN_2576

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

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ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTGCCTGGT
GGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTAGCAGGT
GTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAAGTATTA
GCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAAAGTATT
GCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGC
AATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGACCAAATA
GCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCCATCGTA
TATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATTAGTGAA
GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_RS14780 [old locus tag: NWMN_2576 ]
symbol: NWMN_RS14780
description: histidinol dehydrogenase
length: 416
theoretical pI: 4.80567
theoretical MW: 46177.7
GRAVY: -0.176683

⊟Function[edit | edit source]

reaction:
EC 1.1.1.23? ExPASy
Histidinol dehydrogenase L-histidinol + H₂O + 2 NAD⁺ = L-histidine + 2 NADH
TIGRFAM:
Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
TheSEED: see NWMN_2576
PFAM:
ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 472.1)
and 6 more
no clan defined DUF2004; Protein of unknown function (DUF2004) (PF09406; HMM-score: 14.5)
HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.3)
MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 12.8)
no clan defined YtxH; YtxH-like protein (PF12732; HMM-score: 11.7)
BORCS6; BLOC-1-related complex sub-unit 6 C-terminal helix (PF10157; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Zn²⁺
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9987
- Cytoplasmic Membrane Score: 0.0004
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0007
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001818
- TAT(Tat/SPI): 0.000203
- LIPO(Sec/SPII): 0.000226
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446853387 NCBI
RefSeq: WP_000930643 NCBI
UniProt: see NWMN_2576

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data[edit | edit source]

experimentally validated:
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CodY, HisR* see NWMN_2576

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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