PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2579 [new locus tag: NWMN_RS14795 ]
• pan locus tag^?: SAUPAN006437000
• symbol: NWMN_2579
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2579 [new locus tag: NWMN_RS14795 ]
• symbol: NWMN_2579
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2846938..2848062
• length: 1125
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331603 NCBI
• RefSeq: YP_001333613 NCBI
• BioCyc:
• MicrobesOnline: 3708181 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  1021
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  ATGCATAAAAAATATTTATTGCTATTTTTTCTGTTATTATCAGTTGCATTGTTATATAGC
  TGTTCCAAGCAAAATGAGAAAGCTCAAAATAAATCTGTTAATCCAAATGAGTATGCAAAG
  CATAAATCGAAAATGGATGAACATTGGGAAACATATCACGGCCAAGTTTATCATGTGTTT
  TATCACCCATTAATTACAGACCCAAAAGTTGCGTTTACAGGAGATACACACCAAGCTAAG
  GGCAACAATGATTGGATGATTACTGTAAGTGAATTTAAAAAATCATTGAACGAATTATAT
  AAAAATAATTACATCATCGTTAATCCACATGATGTATACGACTTATCATCTCATCATGTA
  AAAGCCAAAAAAATAAAATTGCCGAAAGGTAAAAAGCCACTAATCATCTCAATTGATGAT
  ATGAATTACTATAGTTACATGAGACACCATGGTTATGCTGACCGTTTAGTACTCGATAAA
  AATAAACATGTTGTTTCTGAAACTACAGACAAAAAAGGCCATGCTACTTATTCTGATGAC
  AATGATATCGTGCCAATTTTAAATAAATTTGTAAAAGAGCATCCAGATTTTTCTTTAAAT
  GGTGAAAAAGGTGTTGTTGCTTTAACCGGTTATGAGGGCGTATTAGGTTATCGCACGAAT
  GAATTAACGAGTAAAGATTATACAAAGAATAAAAAGGCAGCTGAGGAAGTTGTTCGCGCA
  ATGAAACGGGATGGATGGTCATTTGCGAGTCATTCATATGGTCACATTAATTTTGAAAAA
  ACATCTTTAGAAGGTATTAAACGTGATACTAAAAGATGGAAAGATGAAGTTGAACCAATT
  GTAGGTAAAACAGATATGTTTGTATTTCCACATGGCGCACAGGATAGACATACACAAGCG
  TATGATTATTTGGTAGATGAAGCAGAGTTTAAGTTTATTGCAGGTGTCGGTCCAAACAAT
  TTTACAGATATATCAGCAACGAACGTTTACCAAGATAGGGTTGCGATTGATGGTCTAAAC
  TTATTTGAATTTAAATATAAACTCAAACCGTTTTTTAATCCTGAAAATGTATATAGTAAA
  CAAGATAGACGTTACTTTAAAGGGAATCGGGATTATGAAGAATAG
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  1125
  

⊟Protein

⊟General

• locus tag: NWMN_2579 [new locus tag: NWMN_RS14795 ]
• symbol: NWMN_2579
• description: hypothetical protein
• length: 374
• theoretical pI: 9.01084
• theoretical MW: 43552.8
• GRAVY: -0.757754

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination polysaccharide deacetylase family sporulation protein PdaB (TIGR02764; HMM-score: 24.9)
  aromatic cluster surface protein (TIGR04313; HMM-score: 23.3)
  and 4 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
  Cellular processes Cellular processes Sporulation and germination delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide deacetylase family protein, PEP-CTERM locus subfamily (TIGR03006; HMM-score: 16.4)
  putative urate catabolism protein (TIGR03212; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GH_CE (CL0158) Polysacc_deac_1; Polysaccharide deacetylase (PF01522; HMM-score: 31.8)
  and 4 more
  no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 15.1)
  DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 15)
  HicA_toxin; HicA toxin of bacterial toxin-antitoxin, (PF07927; HMM-score: 12.7)
  GH_CE (CL0158) DUF2334; Uncharacterized protein conserved in bacteria (DUF2334) (PF10096; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: ALLYSCS
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.001313
  • TAT(Tat/SPI): 0.000073
  • LIPO(Sec/SPII): 0.998424
  • Cleavage Site: CS pos: 20-21. LYS-CS. Pr: 0.9986
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222791 NCBI
• RefSeq: YP_001333613 NCBI
• UniProt: A0A0H3KFE7 UniProt

⊟Protein sequence

• MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSKMDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKNNYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDKNKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYRTNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDEVEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRVAIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications