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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2591 [new locus tag: NWMN_RS14855 ]
  • pan locus tag?: SAUPAN006450000
  • symbol: NWMN_2591
  • pan gene symbol?: bstA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2591 [new locus tag: NWMN_RS14855 ]
  • symbol: NWMN_2591
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2858136..2858600
  • length: 465
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACTACAAAAACAGTATTTGATGTCATTGATATGGGGTTAGGATATTTAGTAAATGTG
    TATGATGCTTGGAAAGTTGAAAAGGTACTTGATGATTATCATAAGCCTTTTTCTAATACC
    ATTCATTGGCAATTTGGTCATGTATTAACAATTTTTGAATCGGCCTTAGCTGTTGCTGGT
    AAAGAGAATATTGATTTAAATATCTATAGACCTTTATTCGGAAATGGTTCGTCTCCAGAT
    GAATGGAAGGATGAAGTACCGAGTATTGAAAGGATTTTAGAAGGTCTCCAAACTTTACCT
    GAACGTGCACGAAATCTAACTGAAGATGATTTAGCAATTGAATTGAAACAGCCAATTGTC
    GGTTGTAATAACTTAGAAGAGTTATTAGTATTAAATGCCATTCACATCCCACTTCATGCT
    GGTAAAATTGAAGAGATGTCTCGTATATTAAAAAATTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    465

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2591 [new locus tag: NWMN_RS14855 ]
  • symbol: NWMN_2591
  • description: hypothetical protein
  • length: 154
  • theoretical pI: 4.72146
  • theoretical MW: 17511
  • GRAVY: -0.143506

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    DinB (CL0310) DinB_2; DinB superfamily (PF12867; HMM-score: 22.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00392
    • TAT(Tat/SPI): 0.000197
    • LIPO(Sec/SPII): 0.000499
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQFGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPERARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Varahenage R Perera, Gerald L Newton, Kit Pogliano
Bacillithiol: a key protective thiol in Staphylococcus aureus.
Expert Rev Anti Infect Ther: 2015, 13(9);1089-107
[PubMed:26184907] [WorldCat.org] [DOI] (I p)

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