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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
- pan locus tag?: SAUPAN006459000
- symbol: NWMN_2595
- pan gene symbol?: nixA
- synonym:
- product: high-affinity nickel transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
- symbol: NWMN_2595
- product: high-affinity nickel transporter
- replicon: chromosome
- strand: +
- coordinates: 2862506..2863558
- length: 1053
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331617 NCBI
- RefSeq: YP_001333629 NCBI
- BioCyc:
- MicrobesOnline: 3708197 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGATTAAATATCATGTTATGAAAGAAGGATATATTTTGACTGTTTTTAAAAATGAGCGC
TTAAGCTGGTTACCATACATAGCTATTGTCATTTTGTTACACGTTATTGGGTTTAGTTTT
TTATGGATTGCTGGAAAAGACCATCATATCTTATTTGGTATGGGGATTCTTGCATATACA
TTAGGTTTGCGTCATGCATTTGATGCAGATCACATTGCTGCAATAGATAATACGGTTCGC
AAATTATTACAGCAACGCAAAGATCCATCTGGTGTGGGTTTCTATTTTTCAATTGGACAT
TCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGGGTATCTGTAAAATGGGCTAAAGAT
GAATTACCGCATTTCCAAGATATTGGTGGAACGATTGGTACACTAGTTTCAGGTTTCTTT
TTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTAATCTCTTTAATTAACTTGTTCGCT
AAATTACGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGA
TTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTG
CCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCT
CTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATT
TTATTCGCATCAGGTATGAGTTTATTGGATACATTAGATGGTGTTGTAATGAAGTATGCC
TATAATTGGGCATTCTTCAATCCTATTCGCAAAATCTATTACAATATAACGATTACTGCG
ATATCAGTCATGGCAGCATTAGTGATTGGGATGATTGAATTGCTACAAATTCTTGCTGAT
AAGTTAGATTTACATGGTGCGTTTTGGGCATTCATTGGTTCGATCGAATTTGATTATTTA
GGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAG
TTTGGTCGCATAGAACACAAATGGTCTAGATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
- symbol: NWMN_2595
- description: high-affinity nickel transporter
- length: 350
- theoretical pI: 8.66941
- theoretical MW: 39305.3
- GRAVY: 0.743714
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.8)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- ⊞PFAM: LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.2)and 1 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MIKYHVMKEGYILTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p) - ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)