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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman
locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
pan locus tag^?: SAUPAN006459000
symbol: NWMN_2595
pan gene symbol^?: nixA
synonym:
product: high-affinity nickel transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
symbol: NWMN_2595
product: high-affinity nickel transporter
replicon: chromosome
strand: +
coordinates: 2862506..2863558
length: 1053
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 5331617 NCBI
RefSeq: YP_001333629 NCBI
BioCyc:
MicrobesOnline: 3708197 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
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961
1021
ATGATTAAATATCATGTTATGAAAGAAGGATATATTTTGACTGTTTTTAAAAATGAGCGC
TTAAGCTGGTTACCATACATAGCTATTGTCATTTTGTTACACGTTATTGGGTTTAGTTTT
TTATGGATTGCTGGAAAAGACCATCATATCTTATTTGGTATGGGGATTCTTGCATATACA
TTAGGTTTGCGTCATGCATTTGATGCAGATCACATTGCTGCAATAGATAATACGGTTCGC
AAATTATTACAGCAACGCAAAGATCCATCTGGTGTGGGTTTCTATTTTTCAATTGGACAT
TCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGGGTATCTGTAAAATGGGCTAAAGAT
GAATTACCGCATTTCCAAGATATTGGTGGAACGATTGGTACACTAGTTTCAGGTTTCTTT
TTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTAATCTCTTTAATTAACTTGTTCGCT
AAATTACGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGA
TTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTG
CCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCT
CTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATT
TTATTCGCATCAGGTATGAGTTTATTGGATACATTAGATGGTGTTGTAATGAAGTATGCC
TATAATTGGGCATTCTTCAATCCTATTCGCAAAATCTATTACAATATAACGATTACTGCG
ATATCAGTCATGGCAGCATTAGTGATTGGGATGATTGAATTGCTACAAATTCTTGCTGAT
AAGTTAGATTTACATGGTGCGTTTTGGGCATTCATTGGTTCGATCGAATTTGATTATTTA
GGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAG
TTTGGTCGCATAGAACACAAATGGTCTAGATAA
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1053

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
symbol: NWMN_2595
description: high-affinity nickel transporter
length: 350
theoretical pI: 8.66941
theoretical MW: 39305.3
GRAVY: 0.743714

⊟Function[edit | edit source]

TIGRFAM:
Metabolism Transport and binding proteins Cations and iron carrying compounds transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.8)
TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
PFAM:
LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.2)
and 1 more
no clan defined MHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000863
- TAT(Tat/SPI): 0.000086
- LIPO(Sec/SPII): 0.000757
predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

GI: 151222807 NCBI
RefSeq: YP_001333629 NCBI
UniProt: A0A0H3KAZ2 UniProt

⊟Protein sequence[edit | edit source]

MIKYHVMKEGYILTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR

⊟Experimental data[edit | edit source]

experimentally validated:
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

regulator: SigB (activation) regulon
SigB (sigma factor) controlling a large regulon involved in stress/starvation response and adaptation; ^[1] ^[2] other strains

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p)
↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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