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Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
  • symbol: NWMN_2595
  • product: high-affinity nickel transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2862506..2863558
  • length: 1053
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAAATATCATGTTATGAAAGAAGGATATATTTTGACTGTTTTTAAAAATGAGCGC
    TTAAGCTGGTTACCATACATAGCTATTGTCATTTTGTTACACGTTATTGGGTTTAGTTTT
    TTATGGATTGCTGGAAAAGACCATCATATCTTATTTGGTATGGGGATTCTTGCATATACA
    TTAGGTTTGCGTCATGCATTTGATGCAGATCACATTGCTGCAATAGATAATACGGTTCGC
    AAATTATTACAGCAACGCAAAGATCCATCTGGTGTGGGTTTCTATTTTTCAATTGGACAT
    TCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGGGTATCTGTAAAATGGGCTAAAGAT
    GAATTACCGCATTTCCAAGATATTGGTGGAACGATTGGTACACTAGTTTCAGGTTTCTTT
    TTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTAATCTCTTTAATTAACTTGTTCGCT
    AAATTACGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGA
    TTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTG
    CCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCT
    CTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATT
    TTATTCGCATCAGGTATGAGTTTATTGGATACATTAGATGGTGTTGTAATGAAGTATGCC
    TATAATTGGGCATTCTTCAATCCTATTCGCAAAATCTATTACAATATAACGATTACTGCG
    ATATCAGTCATGGCAGCATTAGTGATTGGGATGATTGAATTGCTACAAATTCTTGCTGAT
    AAGTTAGATTTACATGGTGCGTTTTGGGCATTCATTGGTTCGATCGAATTTGATTATTTA
    GGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAG
    TTTGGTCGCATAGAACACAAATGGTCTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
  • symbol: NWMN_2595
  • description: high-affinity nickel transporter
  • length: 350
  • theoretical pI: 8.66941
  • theoretical MW: 39305.3
  • GRAVY: 0.743714

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.2)
    and 1 more
    no clan defined MHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000863
    • TAT(Tat/SPI): 0.000086
    • LIPO(Sec/SPII): 0.000757
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIKYHVMKEGYILTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: SigB (activation) regulon

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]