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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2630 [new locus tag: SAUSA300_RS14610 ]
  • pan locus tag?: SAUPAN006459000
  • symbol: nixA
  • pan gene symbol?: nixA
  • synonym:
  • product: high-affinity nickel-transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2630 [new locus tag: SAUSA300_RS14610 ]
  • symbol: nixA
  • product: high-affinity nickel-transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2856442..2857458
  • length: 1017
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGACTGTTTTTAAAAATGAGCGCTTAAGCTGGTTACCATACATAGCTATTGTCATTTTG
    TTACACGTTATTGGGTTTAGTTTTTTATGGATTGCTGGAAAAGACCATCATATCTTATTT
    GGTATGGGGATTCTTGCATATACATTAGGTTTGCGTCATGCATTTGATGCAGATCACATT
    GCTGCAATAGATAATACGGTTCGCAAATTATTACAGCAACGCAAAGATCCATCTGGTGTG
    GGTTTCTATTTTTCAATTGGACATTCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGG
    GTATCTGTAAAATGGGCTAAAGATGAATTACCGCATTTCCAAGATATTGGTGGAACGATT
    GGTACACTAGTTTCAGGTTTCTTTTTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTA
    ATCTCTTTAATTAACTTGTTCGCTAAATTACGTCGTGAACACATTGAAGAAGCTGAAGTC
    GATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTA
    ATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACA
    GCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTT
    ATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTATTGGATACATTA
    GATGGTGTTGTAATGAAGTATGCCTATAATTGGGCATTCTTCAATCCTATTCGCAAAATC
    TATTACAATATAACGATTACTGCGATATCAGTCATGGCAGCATTAGTGATTGGGATGATT
    GAATTGCTACAAATTCTTGCTGATAAGTTAGATTTACATGGTGCGTTTTGGGCATTCATT
    GGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGG
    CTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1017

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2630 [new locus tag: SAUSA300_RS14610 ]
  • symbol: NixA
  • description: high-affinity nickel-transporter
  • length: 338
  • theoretical pI: 8.02909
  • theoretical MW: 37829.5
  • GRAVY: 0.765976

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.9)
  • TheSEED  :
    • HoxN/HupN/NixA family nickel/cobalt transporter
    Membrane Transport Membrane Transport - no subcategory Transport of Nickel and Cobalt  HoxN/HupN/NixA family nickel/cobalt transporter
  • PFAM:
    LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.3)
    and 1 more
    no clan defined MHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008354
    • TAT(Tat/SPI): 0.000304
    • LIPO(Sec/SPII): 0.002886
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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