PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2599 [new locus tag: NWMN_RS14895 ]
• pan locus tag^?: SAUPAN006466000
• symbol: vraE
• pan gene symbol^?: vraE
• synonym:
• product: ABC transporter permease, involved in vancomycin resistance

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2599 [new locus tag: NWMN_RS14895 ]
• symbol: vraE
• product: ABC transporter permease, involved in vancomycin resistance
• replicon: chromosome
• strand: +
• coordinates: 2866272..2868152
• length: 1881
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332598 NCBI
• RefSeq: YP_001333633 NCBI
• BioCyc:
• MicrobesOnline: 3708201 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACATTTAACCATATCGTTTTCAAAAACTTACGACAAAACTTAAAACATTATGCAATG
  TATTTATTTTCATTATTTTTTAGCATCGTCTTATATTTCAGTTTTACAACCTTACAGTTT
  ACTAAAGGTGTAAATAATGACGACTCGATGGCCATCATTAAGAAAGGTGCTTTAGTCGGA
  TCAATATTTTTATTTATCATTATTGTCATCTTTTTAATGTATGCCAATCATTTATTCGTA
  AAACGCCGTACACGTGAATTTGCGCTATTTCAGTTGATTGGTTTAACACGACAAAACATT
  TTGAAAATGTTAGCACTTGAACAAATGATCGTATTTTTAATCACAGGTGTTGTCGGTGTA
  TTATGTGGCATTGCAGGTGCACAATTATTGCTTTCCATAGTATCTAAATTGATGTCGTTA
  TCGATTAACTTATCGATACATTTCGAACCTATGGCCCTTGTTTTAACTATTTTCATGCTA
  ATTATTGCGTATGTACTGATTTTATTTAAAAGTGCTTTATTTCTAAAAAGACGTAGTATC
  TTATCAATGATGAAAGATTCAATTAAAACTGATGCCACAACTGCTAAAGTAACGACTGCA
  GAGGTTATTTCAGGCGTATTAGGTATTGCTATGATTGCACTAGGCTATTATATGGCGACA
  GAAATGTTTGGTACGTTCAAAGCACTAACTATGGCTATGACATCACCGTTTATCATTTTA
  TTTTTAACGGTTGTAGGCGCCTATTTATTCTTCAGAAGTTCCGTGTCACTTATTTTTAAA
  ACATTGAAAAAATCAAAAAATGGACGCGTATCTATTACCGATGTTGTATTCACATCGTCT
  ATTATGTACAGAATGAAGAAAAATGCCATGTCTTTAACTATCATTGCAATTATTTCTGCA
  GTTACAGTAACTGTATTGTGTTTTGCGGCATTATCTAAATCAAATACAGATCAAACCCTT
  ACATCTATGGCACCAAATGAATTTAACGTGGTCGCTACTCAAGATGCTAAACAATTTGAA
  ACTAAACTAAGCCAACAACAAATTACATTTTCTAAAAATGCTTACGAAACTATTACTGTA
  GATAATGTTAATGATCAGGTTATTACTTTGGAAAATGGTAGTGATTCAGGTCGCACGAAT
  TCTATTTTAAGTGCAAATAACAAGTTAACAGGCAACAATGCCATCATTACAAATACAAAA
  TCGCTGCCTAACATTATTAATATCCATTTAAACAAAGATTTAGTAGTAAAAGGTACTAAG
  AATGAAACTTTCCGTGTTACACAAGAAGACAAAGGTAAGGTTTATCCTTTAAATCTAAGC
  TTCAACTCACCTGTCATCGAAGTAAGTCCTGAAAAGTATCAGCAGTTGAAAACACAAAAT
  AACGTTCATACTTTTTATGGATATGATATTAAACAAACATCACAAAAGGAAAAAGCTCAA
  GCAATTGCAAAACAGTTTGGAGACAAAGTCATCACGTATGATGAAATGAAAAAAGAGGTC
  GATGCTACTAACGGTATTCTAATATTTGTTACATCATTTTTAGGATTAGCATTTTTAGTA
  GCAGCTGGATGTATTATTTATATTAAACAAATGGATGAAACTGAGGATGAACTAAGTAAC
  TTCAGAATATTAAAACGCATAGGCTTTACGCATACAGATATGCTTAAGGGATTATTATTA
  AAAATTACTTTCAACTTCGGTTTACCATTATTGATTGCTATATTACATGCAGTATTCGCC
  GCAATAGCATTTATGAAATTGATGGGTAATATTTCCTTCATGCCGGTTATTGTAGTAATT
  GTTGTATACACCCTCATTTACATTACTTTTGCTTTAATTGCTTTTGTACATTCAAACAAA
  TTGATTAAGAAAACCATTTAA
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⊟Protein

⊟General

• locus tag: NWMN_2599 [new locus tag: NWMN_RS14895 ]
• symbol: VraE
• description: ABC transporter permease, involved in vancomycin resistance
• length: 626
• theoretical pI: 10.2122
• theoretical MW: 69992.7
• GRAVY: 0.496326

⊟Function

• TIGRFAM:
  acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 19.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 41.4)
  and 3 more
  Yip1 (CL0112) Yip1; Yip1 domain (PF04893; HMM-score: 10.3)
  no clan defined MLANA; Protein melan-A (PF14991; HMM-score: 9.6)
  DUF2855; Protein of unknown function (DUF2855) (PF11017; HMM-score: 8.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009921
  • TAT(Tat/SPI): 0.000156
  • LIPO(Sec/SPII): 0.006009
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222811 NCBI
• RefSeq: YP_001333633 NCBI
• UniProt: A0A0H3KJB1 UniProt

⊟Protein sequence

• MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGVNNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILKMLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFMLIIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYMATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVVFTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQDAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGNNAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSPEKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILIFVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNFGLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKKTI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: vraD > vraE > NWMN_2600 > NWMN_2601

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications