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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA2493 [new locus tag: SA_RS14255 ]
- pan locus tag?: SAUPAN006466000
- symbol: vraE
- pan gene symbol?: vraE
- synonym:
- product: ABC transporter permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA2493 [new locus tag: SA_RS14255 ]
- symbol: vraE
- product: ABC transporter permease
- replicon: chromosome
- strand: +
- coordinates: 2803941..2805821
- length: 1881
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1125423 NCBI
- RefSeq: NP_375820 NCBI
- BioCyc: see SA_RS14255
- MicrobesOnline: 104846 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1861ATGACATTTAACCATATCGTTTTCAAAAACTTACGACAAAACTTAAAACATTATGCAATG
TATTTATTTTCATTATTTTTTAGCATCGTCTTATATTTCAGTTTTACAACCTTACAGTTT
ACTAAAGGTGTAAATAATGACGACTCGATGGCCATCATTAAGAAAGGTGCTTTAGTCGGA
TCAATATTTTTATTTATCATTATTGTCATCTTTTTAATGTATGCCAATCATTTATTCGTG
AAACGCCGTACACGTGAATTTGCGCTATTTCAGTTGATTGGTTTAACACGACAAAACATT
TTGAAAATGTTAGCACTTGAACAAATGATTGTATTTTTAATCACAGGTGTTGTCGGTGTA
TTATGTGGCATTGCAGGTGCACAATTATTGCTTTCCATAGTATCTAAATTGATGTCGTTA
TCGATTAACTTATCGATACATTTCGAACCTATGGCCCTTGTTTTAACTATTTTCATGCTA
ATTATTGCGTATGTACTGATTTTATTTCAAAGTGCTTTATTTCTAAAAAGACGTAGTATC
TTATCAATGATGAAAGATTCAATTAAAACTGATGCCACAACTGCTAAAGTAACGACTGCA
GAGGTTATTTCAGGCGTATTAGGTATTGCTATGATTGCACTAGGCTATTATATGGCGACA
GAAATGTTTGGAACATTTAAAGCACTAACTATGGCTATGACATCACCGTTTATCATTTTA
TTTTTAACGGTAGTAGGCGCCTATTTATTCTTCAGAAGTTCCGTGTCACTAATTTTTAAA
ACTTTGAAAAAATCTAAAAATGGACGCGTATCTATTACAGATGTTGTATTTACATCGTCT
ATTATGTACCGAATGAAGAAAAATGCGATGTCTTTAACTATCATTGCAATTATTTCTGCA
GTTACAGTAACTGTATTGTGTTTCGCGGCATTATCTAAATCAAATACAGATCAAACCCTT
ACATCTATGGCACCAAATGAATTTAACGTGGTCGCTACTCAAGATGCTAAACAATTTGAA
ACTAAACTAAGCCAACAACAAATTACATTTTCTAAAAATGCTTACGAAACTATTACTGTA
GATAATGTTAAAGATCAGGTTATTACTTTGGAAAATGGTAGTGATTCAGGTCGCACGAAT
TCAATATTAAGTGCAAATAACAAGGTAACAGGCAACAATGCCATCATTACAAATACAAAA
TCACTTCCTAACATTATTAATATCCATTTAAACAAAGATTTAGTAGTAAAAGGAACTAAG
AATGAAACTTTCCGTGTTACACAAGAAGACAAAGGTAGGGTTTATCCTTTAAATCTAAGC
TTCAACTCACCTGTCGTCGAAGTAAGTCCTGAAAAGTATCAGCAGTTGAAAACACAAAAT
AACGTTCATACTTTTTATGGATATGATATTAAACAAACATCACAAAAGGAAAAAGCTCAA
GCAATTGCAAAACAGTTTGGAGACAAAGTCATCACTTATGATGAAATGAAAAAAGAGGTC
GATGCTACTAACGGTATTCTAATATTTGTTACATCATTTTTAGGATTAGCATTTTTAGTA
GCAGCTGGATGTATTATTTATATTAAACAAATGGATGAAACTGAGGATGAACTAAGTAAC
TTCAGAATATTAAAACGCATAGGCTTTACGCATACAGATATGCTTAAGGGATTATTATTA
AAAATTACTTTCAACTTCGGTTTACCATTATTGATTGCTATATTACATGCAGTATTCGCC
GCAATAGCATTTATGAAATTGATGGGTAATATTTCCTTCATGCCGGTTATTGTAGTAATT
GTTGTATACACCCTCATTTACATTACTTTTGCTTTAATTGCTTTTGTACATTCAAACAAA
TTGATTAAGAAAACCATTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA2493 [new locus tag: SA_RS14255 ]
- symbol: VraE
- description: ABC transporter permease
- length: 626
- theoretical pI: 10.2193
- theoretical MW: 70006.7
- GRAVY: 0.495527
⊟Function[edit | edit source]
- TIGRFAM: acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 19.1)
- TheSEED :
- ABC transporter-like sensor and permease protein SERP0020
- PFAM: BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 40.5)and 3 moreno clan defined MLANA; Protein melan-A (PF14991; HMM-score: 9.6)Yip1 (CL0112) Yip1; Yip1 domain (PF04893; HMM-score: 9.4)no clan defined DUF2855; Protein of unknown function (DUF2855) (PF11017; HMM-score: 7.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 10
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009921
- TAT(Tat/SPI): 0.000156
- LIPO(Sec/SPII): 0.006009
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGVNNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILKMLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFMLIIAYVLILFQSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYMATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVVFTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQDAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKVTGNNAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGRVYPLNLSFNSPVVEVSPEKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILIFVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNFGLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKKTI
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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