PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS00040 [old locus tag: NWMN_0007 ]
• pan locus tag^?: SAUPAN000014000
• symbol: NWMN_RS00040
• pan gene symbol^?: hutH
• synonym:
• product: histidine ammonia-lyase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS00040 [old locus tag: NWMN_0007 ]
• symbol: NWMN_RS00040
• product: histidine ammonia-lyase
• replicon: chromosome
• strand: +
• coordinates: 10900..12414
• length: 1515
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (10900..12414) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0007

⊟Phenotype

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⊟DNA sequence

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  ATGACTTTATATTTAGATGGTGAAACACTAACAATTGAGGATATTAAATCATTTTTACAA
  CAACAATCAAAGATTGAAATTATTGATGATGCGTTAGAACGTGTCAAAAAAAGTAGAGCG
  GTAGTTGAACGTATTATTGAAAATGAGGAAACGGTTTACGGTATTACTACAGGTTTTGGG
  TTATTTAGTGATGTACGTATAGACCCGACGCAATATAATGAATTACAAGTGAATCTGATA
  CGCTCACATGCCTGTGGACTAGGTGAGCCATTTTCAAAAGAAGTAGCATTAGTCATGATG
  ATTTTACGATTGAATACATTATTAAAAGGTCATTCAGGTGCCACTTTAGAATTAGTGAGA
  CAATTACAATTTTTTATAAATGAACGTATTATACCGATAATCCCACAGCAAGGCTCTCTC
  GGTGCATCAGGAGATTTAGCGCCATTATCACATTTAGCATTAGCATTAATTGGTGAAGGG
  AAAGTATTGTACAGAGGGGAAGAAAAGGATAGTGACGATGTATTAAGAGAATTAAATAGA
  CAACCTTTGAACCTTCAGGCTAAAGAAGGTTTAGCATTGATTAATGGTACGCAAGCTATG
  ACAGCTCAAGGTGTCATTAGTTATATAGAAGCAGAAGATTTAGGTTACCAATCTGAATGG
  ATTGCTGCATTAACGCATCAGTCTCTTAATGGCATTATAGATGCATATCGACATGATGTG
  CACGCAGTTCGTAATTTTCAAGAACAGATTAATGTGGCAGCGCGTATGCGTGATTGGTTA
  GAAGGATCAACATTAACGACGCGACAATCAGAAATACGTGTACAAGATGCATATACGTTG
  CGTTGTATACCACAAATCCATGGCGCGAGTTTTCAAGTATTCAATTATGTTAAACAGCAA
  TTAGAATTTGAAATGAATGCGGCTAATGATAATCCACTTATATTTGAGGAAGCAAATGAA
  ACGTTTGTTATTTCAGGTGGTAACTTCCATGGACAACCTATTGCTTTTGCATTAGATCAT
  CTTAAATTAGGTGTAAGTGAATTAGCAAACGTATCGGAACGTCGTCTAGAGCGACTAGTA
  AATCCTCAATTAAATGGTGATTTACCAGCATTTCTTAGTCCAGAGCCAGGATTGCAAAGT
  GGCGCGATGATTATGCAATATGCTGCTGCAAGTCTCGTTTCTGAAAATAAAACTTTAGCG
  CATCCAGCGAGTGTTGATTCTATCACTTCATCTGCGAACCAAGAAGATCACGTATCTATG
  GGAACTACAGCTGCTAGACATGGTTATCAAATTATTGAAAATGCAAGACGTGTGCTGGCA
  ATCGAATGTGTTATTGCATTACAAGCAGCAGAGTTAAAAGGTGTTGAAGGATTATCACCA
  AAAACACGTCGCAAGTATGATGAGTTTCGAAGTATCGTGCCATCCATTACACATGATCGT
  CAATTTCATAAAGATATTGAAGCGGTTGCACAGTATTTAAAGCAATCAATTTATCAAACG
  ACTGCATGTCACTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS00040 [old locus tag: NWMN_0007 ]
• symbol: NWMN_RS00040
• description: histidine ammonia-lyase
• length: 504
• theoretical pI: 5.14671
• theoretical MW: 56075.2
• GRAVY: -0.185714

⊟Function

• reaction:
  EC 4.3.1.3?  ExPASy
  Histidine ammonia-lyase L-histidine = urocanate + NH₃
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines histidine ammonia-lyase (TIGR01225; EC 4.3.1.3; HMM-score: 665)
  and 4 more
  tyrosine 2,3-aminomutase (TIGR03832; EC 5.4.3.6; HMM-score: 442.9)
  phenylalanine aminomutase (D-beta-phenylalanine forming) (TIGR04475; EC 5.4.3.11; HMM-score: 397.1)
  phenylalanine ammonia-lyase (TIGR01226; EC 4.3.1.24; HMM-score: 269.2)
  phenylalanine aminomutase (L-beta-phenylalanine forming) (TIGR04473; EC 5.4.3.10; HMM-score: 144.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined Lyase_aromatic; Aromatic amino acid lyase (PF00221; HMM-score: 612.4)
  and 2 more
  UPF0223; Uncharacterised protein family (UPF0223) (PF05256; HMM-score: 14.4)
  RNR_N; Ribonucleotide reductase N-terminal (PF08343; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00164
  • TAT(Tat/SPI): 0.00019
  • LIPO(Sec/SPII): 0.00027
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446099609 NCBI
• RefSeq: WP_000177464 NCBI
• UniProt: see NWMN_0007

⊟Protein sequence

• MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVERIIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMILRLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEGKVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQSEWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQDAYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPIAFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASLVSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAAELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see NWMN_0007

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications