PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS00805 [old locus tag: NWMN_0147 ]
• pan locus tag^?: SAUPAN001060000
• symbol: NWMN_RS00805
• pan gene symbol^?: ggt
• synonym:
• product: gamma-glutamyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS00805 [old locus tag: NWMN_0147 ]
• symbol: NWMN_RS00805
• product: gamma-glutamyltransferase
• replicon: chromosome
• strand: +
• coordinates: 186405..188411
• length: 2007
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (186405..188411) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0147

⊟Phenotype

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⊟DNA sequence

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  ATGGTCATTAACTTAAATGACAAACAGACAAAAACATCTAAAGAAGGGTTAATTTCCGTA
  TCACATCCTCTTGCGGCTAAAATTGGTAAGGATGTATTAGATCAAGGTGGCAACGCCATG
  GATGCAGTGATTGCAATTCAACTGGCATTGAATGTGGTAGAACCATTTGCATCAGGTATT
  GGTGGTGGCGGGTATTTGCTATATTATGAGCAAAGTACTGGCAGTATAACTGCGTTTGAT
  GCACGTGAGACAGCACCTGAACATGTAGACAAACAATTTTATCTAGATGATTCAGGCGAA
  TATAAATCATTTTTTGATATGACTACACATGGTAAAACTGTCGCTGTGCCAGCAATTCCA
  AAGCTGTTTGATTATATTCACAAGCGTTATGCTAAATTGTCATTGGAAGATTTAATTAAT
  CCTGCAATTGAACTAGCCATTGAAGGTCATGCAGCCAATTGGGCTACTGAAAAATATTCG
  CGCCAGCAACACGCACGATTGACAAAGTATCATGAAACGGCACAAGTATTTACGCATGAA
  AATCAATATTGGCGTGAAGGTGATTGGATTGTACAACCCGAATTAGGTAAGACATTTCAA
  ATATTAAGAGAACAAGGGTTTAATGCATTTTATAAAGGTGACATTGCGAAACAATTAGTC
  AATGTTGTCAAAGCATGTGGTGGGACAATCACTTTAGAGGATCTAGCCAAATATGACATT
  CAGATTAAAGCGCCAATCAGTGCAACATTTAAAGACTATGACATTTATTCAATGGGACCA
  TCTAGTTCTGGCGGTATCACGGTAATTCAAATATTGAAGTTATTAGAACATGTCGATTTA
  CCATCTATGGGTCCAAGATCTGTTGATTACTTGCATCATTTGATACAAGCGATGCATTTA
  GCATATAGTGATCGTGCGCAATACTTGGCGGATGATAATTTTCATGAGGTGCCTGTACAG
  TCATTAATTGATGATGATTATTTAAAAGCACGCAGTACGCTCATTGATAGCAATAAAGCA
  AATATTGATATAGAGCATGGTGTTGTGTCTGATTGCATTAGTCATACAGATGTTGAAGAA
  AATCATACCGAAACAACTCATTTTTGTGTGATTGATAAGGAAGGTAATATTGCTTCATTT
  ACGACATCAATTGGTATGATTTATGGTTCAGGTATCACGATTCCAGGCTACGGTGTGTTA
  TTGAATACGACAATGGTTGGCTTTGATGTAGTAGATGGTGGTATTAACGAAATTGCACCA
  TATAAACGACCACTAAGTAACATGGCTCCAACGATTGTGATGTATCACGGGAAGCCAATA
  TTAACAGTAGGTGCACCTGGTGCCATAAGTATCATTGCTAGTGTTGCGCAAACATTAATC
  AATGTATTAGTGTTTGGCATGGATATTCAGCAGGCTATAGATGAACCTAGAATTTATAGT
  AGCCATCCTAATCGCATTGAATGGGAGCCTCAATTTTCACAATCTACAATATTAGCATTG
  ATTGCACATGGACATGCAATGGAACATAAACCAGATGCTTATATTGGAGATGTACATGGA
  CTACAGGTTGACCCAACAACGTATGAAGCTTCGGGTGGAAGTGATGATACTAGAGAAGGC
  ACCGTAATGGGTGGCGAGGTGTTAGTGATTAGAAAACAGCCATTACCATATCGGCAAATG
  TATGATAGTGACGGCTTTCGTCTATATTTCAATGATGTGCAGTTACCTTTATTGGCAGAT
  CAAGTGCGATGGATGCATGACAAATATTGGGTTGATGAAAGTGTTGTTAGAATCATTTTC
  CCTGAAGTTAGTGCACATATTGAAGATTTAAGAAGTTATGAAAACGCAGGAGAAAATTAT
  ATAGATATTGCCTGGTTAGCACGGAAATATGCTTATCAAGTAACATTGAAGGATGACGGT
  TTATACTTAACTGATGATACATATACTTCAGTGAAACGGAACACAAACGCATACTATAGA
  TATGATCGAGATAGTATCACAAGATAG
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⊟Protein

⊟General

• locus tag: NWMN_RS00805 [old locus tag: NWMN_0147 ]
• symbol: NWMN_RS00805
• description: gamma-glutamyltransferase
• length: 668
• theoretical pI: 4.95757
• theoretical MW: 74677.7
• GRAVY: -0.246257

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs gamma-glutamyltransferase (TIGR00066; EC 2.3.2.2; HMM-score: 502.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NTN (CL0052) G_glu_transpept; Gamma-glutamyltranspeptidase (PF01019; HMM-score: 548.6)
  and 1 more
  Asparaginase_2; Asparaginase (PF01112; HMM-score: 16.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.1
  • Cytoplasmic Membrane Score: 0.14
  • Cellwall Score: 0.15
  • Extracellular Score: 9.6
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007882
  • TAT(Tat/SPI): 0.000284
  • LIPO(Sec/SPII): 0.000915
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446158574 NCBI
• RefSeq: WP_000236429 NCBI
• UniProt: see NWMN_0147

⊟Protein sequence

• MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVIAIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYKSFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYSRQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQLVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLEHVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTLIDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGITIPGYGVLLNTTMVGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAISIIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAMEHKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGFRLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIAWLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications