PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
• pan locus tag^?: SAUPAN002337000
• symbol: NWMN_RS03040
• pan gene symbol^?: sdrE
• synonym:
• product: MSCRAMM family adhesin SdrE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
• symbol: NWMN_RS03040
• product: MSCRAMM family adhesin SdrE
• replicon: chromosome
• strand: +
• coordinates: 609219..612719
• length: 3501
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (609219..612719) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0525

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTAACAGGGATAATAAAAAGGCAATAACAAAAAAGGGTATGATTTCAAATCGCTTA
  AACAAATTTTCGATTAGAAAGTATACTGTAGGAACTGCATCGATTTTAGTAGGTACGACA
  TTGATTTTTGGTCTAGGGAACCAAGAAGCTAAAGCTGCTGAAAACACTAGTACAGAAAAT
  GCAAAACAAGATGATGCAACGACTAGTGATAATAAAGAAGTAGTGTCGGAAACTGAAAAT
  AATTCGACAACAGAAAATAATTCAACAAATCCAATTAAGAAAGAAACAAATACTGATTCA
  CAACCAGAAGCTAAAAAAGAATCAACTTCATCAAGTACTCAAAAACAGCAAAATAACGTT
  ACAGCTACAACTGAAACTAAGCCTCAAAACATTGAAAAAGAAAATGTTAAACCTTCAACT
  GATAAAACTGCGACAGAAGATACATCTGTTATTTTAGAAGAGAAGAAAGCACCAAATAAT
  ACAAATAACGATGTAACTACAAAACCATCTACAAGTGAACCATCTACAAGTGAAATTCAA
  ACAAAACCAACTACACCTCAAGAATCTACAAATATTGAAAATTCACAACCGCAACCAACG
  CCTTCAAAAGTAGACAATCAAGTTACAGATGCAACTAATCCAAAAGAACCAGTAAATGTG
  TCAAAAGAAGAACTTAAAAATAATCCTGAGAAATTAAAAGAATTGGTTAGAAATGATAGC
  AATACAGATCATTCAACTAAACCAGTTGCTACAGCTCCAACAAGTGTTGCACCAAAACGT
  GTAAACGCAAAAATGCGCTTTGCAGTTGCACAACCAGCAGCAGTTGCTTCAAACAATGTA
  AATGATTTAATTAAAGTGACGAAGCAAACAATCAAAGTTGGCGATGGTAAAGATAATGTG
  GCAGCAGCGCATGACGGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAA
  GTCAAAAAAGGCGATACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTA
  ACAGATAAAAATGATCCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCTAAAGGA
  ACATTTGATAAAGCAACTAAGCAAATCACATATACATTTACAGACTATGTAGATAAATAT
  GAAGATATAAAATCACGCTTAACTCTATATTCGTATATTGATAAAAAAACAGTTCCAAAT
  GAGACAAGTTTGAATTTAACATTTGCTACAGCAGGTAAAGAAACAAGCCAAAATGTCACT
  GTTGATTATCAAGATCCAATGGTCCATGGTGATTCAAACATTCAATCTATCTTTACAAAA
  TTAGATGAAGATAAGCAAACTATTGAACAACAAATTTATGTTAACCCATTGAAAAAATCA
  GCAACCAACACTAAAGTTGATATAGCTGGTAGTCAAGTAGATGATTATGGAAATATTAAA
  CTAGGAAATGGTAGCACCATTATTGACCAAAATACAGAAATAAAGGTTTATAAAGTTAAC
  TCTGATCAACAATTGCCTCAAAGTAATAGAATCTATGATTTTAGTCAATACGAAGATGTA
  ACAAGTCAATTTGATAATAAAAAATCATTTAGTAATAATGTAGCAACATTGGATTTTGGT
  GATATTAATTCAGCCTATATTATCAAAGTTGTTAGTAAATATACACCTACATCAGATGGC
  GAACTAGATATTGCCCAAGGTACTAGTATGAGAACAACTGATAAATATGGTTATTATAAT
  TATGCAGGATATTCAAACTTCATCGTAACTTCTAATGACACTGGCGGTGGCGACGGTACT
  GTTAAACCTGAAGAAAAGTTATACAAAATTGGTGACTATGTATGGGAAGACGTTGATAAA
  GACGGTGTTCAAGGTACAGATTCAAAAGAAAAACCAATGGCAAACGTTTTAGTTACATTA
  ACTTACCCGGACGGTACTACAAAATCAGTAAGAACAGATGCTAATGGTCATTATGAATTC
  GGTGGTTTGAAAGACGGAGAAACTTATACAGTTAAATTCGAAACGCCAACTGGATATCTT
  CCAACAAAAGTAAATGGAACAACTGATGGTGAAAAAGACTCAAATGGTAGTTCGGTTACT
  GTTAAAATTAATGGTAAAGATGATATGTCTTTAGATACTGGTTTTTACAAAGAACCTAAA
  TACAACTTAGGTGACTATGTATGGGAAGATACTAATAAAGATGGTATCCAAGATGCAAAT
  GAGCCAGGAATCAAAGATGTTAAGGTTACATTAAAAGATAGTACTGGAAAAGTTATTGGT
  ACAACTACTACTGATGCCTCGGGTAAATATAAATTTACAGATTTAGATAATGGTAACTAT
  ACAGTAGAATTTGAAACACCAGCAGGTTACACGCCAACGGTTAAAAATACTACAGCTGAT
  GATAAAGATTCTAATGGTTTAACAACAACAGGTGTCATTAAAGATGCAGATAATATGACA
  TTAGACAGGGGTTTCTATAAAACACCAAAATACAGTTTAGGTGATTATGTTTGGTACGAC
  AGTAATAAAGACGGCAAACAAGATTCAACTGAAAAAGGTATCAAAGATGTGACAGTTACA
  TTGCAAAACGAAAAAGGCGAAGTAATTGGAACAACTAAAACAGATGAAAATGGTAAATAT
  CGTTTCGATAATTTAGATAGCGGTAAATACAAAGTTATTTTTGAAAAGCCTGCTGGCTTA
  ACACAAACAGTTACAAATACAACTGAAGATGATAAAGATGCAGATGGTGGCGAAGTTGAC
  GTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACA
  TCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGAC
  TCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAT
  TCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAC
  AGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGAC
  TCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAT
  TCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAT
  AGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAC
  TCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAC
  AGCGATTCAGACTCGGATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAA
  GACCATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAAT
  AACGCAACGTTATTTGGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGT
  CGCAAAAAACAAAACAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS03040 [old locus tag: NWMN_0525 ]
• symbol: NWMN_RS03040
• description: MSCRAMM family adhesin SdrE
• length: 1166
• theoretical pI: 4.02054
• theoretical MW: 126548
• GRAVY: -1.10214

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 50.1)
  and 2 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 16.2)
  Por secretion system C-terminal sorting domain (TIGR04183; HMM-score: 14.4)
• TheSEED: data available for COL, N315, USA300_FPR3757
• PFAM:
  Transthyretin (CL0287) SdrD_B; SdrD B-like domain (PF17210; HMM-score: 251.6)
  and 14 more
  CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 105.7)
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 104.1)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.4)
  E-set (CL0159) MG1; Alpha-2-macroglobulin MG1 domain (PF11974; HMM-score: 44.9)
  no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 42.6)
  Transthyretin (CL0287) MetallophosN; N terminal of Calcineurin-like phosphoesterase (PF16371; HMM-score: 41.2)
  CarbopepD_reg_2; CarboxypepD_reg-like domain (PF13715; HMM-score: 30)
  DUF2012; Protein of unknown function (DUF2012) (PF09430; HMM-score: 22.9)
  DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 17.8)
  no clan defined CompInhib_SCIN; Staphylococcal complement inhibitor SCIN (PF11546; HMM-score: 16.4)
  GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 14.1)
  Transthyretin (CL0287) DUF1416; Protein of unknown function (DUF1416) (PF07210; HMM-score: 14)
  Dioxygenase_C; Dioxygenase (PF00775; HMM-score: 13.2)
  OB (CL0021) DUF4539; Domain of unknown function (DUF4539) (PF15072; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 52 aa
  • SP(Sec/SPI): 0.83357
  • TAT(Tat/SPI): 0.075298
  • LIPO(Sec/SPII): 0.002832
  • Cleavage Site: CS pos: 52-53. AKA-AE. Pr: 0.8729
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446532960 NCBI
• RefSeq: WP_000610306 NCBI
• UniProt: see NWMN_0525

⊟Protein sequence

• MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQPEAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNTNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTKVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTADDKDSNGLTTTGVIKDADNMTLDRGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications