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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03215 [old locus tag: NWMN_0561 ]
  • pan locus tag?: SAUPAN002451000
  • symbol: NWMN_RS03215
  • pan gene symbol?:
  • synonym:
  • product: DUF443 domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03215 [old locus tag: NWMN_0561 ]
  • symbol: NWMN_RS03215
  • product: DUF443 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 645157..645798
  • length: 642
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_009641 (645157..645798) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    GTGGAGATATTGCTTTGCGAGGTTAGAGTTGTAAACAAAAATCCCAGATATAGAATTATT
    AAATATAAAAACGACTATCTGATGATTGATTTAGTAAGCACCTGGTTAGTTTTGTTCTTT
    CCGTTTATTAATTGGTTAATACCAAAAAAATATGTCCAAATCAGCAGAGAAGATTTTGAT
    AATTTAAATATTGTCAAACCAGTTAAAAATAAAGCTTTATGGCCAGCTATAGGTAGCATA
    CTTTTATTTGGCACTATGTTTAGAGATAAAATATATATACCTGATTCTCATTTAGAAAAA
    AATTGCGTTATTATCATTTGTTCGGTTTTATTATTAAGTATTCTAGTGTTTTATATATAT
    TTAAATCAGAAAGTAAAGTTATCTATCTATAATGATCGGAGTAGCAATGGAAAAATTATG
    ATTTTTCCATCATTCAAGAATCTTTGCTTTGTACTATTTTCTTATTTTTTCTGTGGTGGA
    TTATCAATCATGTTCTTAGATGTTTTGATTAGTTTATCTATCCAAAATATTATAGTATTT
    ATTGCTTGGGTTATTATGACAATGCTATTTTTCTTTATAAATATGTCTTCAATAATAGAC
    AAAAAGATTCATGTTATATATTTAAGGTCATATAAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS03215 [old locus tag: NWMN_0561 ]
  • symbol: NWMN_RS03215
  • description: DUF443 domain-containing protein
  • length: 213
  • theoretical pI: 9.77316
  • theoretical MW: 25226.4
  • GRAVY: 0.659155

Function[edit | edit source]

  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 173)
    and 1 more
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage holin, LL-H family (TIGR01673; HMM-score: 5.7)
  • TheSEED:
  • PFAM:
    no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 222.7)
    and 2 more
    IgaA; Intracellular growth attenuator protein IgaA (PF07095; HMM-score: 13.4)
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002656
    • TAT(Tat/SPI): 0.000113
    • LIPO(Sec/SPII): 0.000559
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000404342 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFINWLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNCVIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGGLSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]