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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03385 [old locus tag: NWMN_0593 ]
  • pan locus tag?: SAUPAN002493000
  • symbol: NWMN_RS03385
  • pan gene symbol?: mnhA2
  • synonym:
  • product: cation:proton antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03385 [old locus tag: NWMN_0593 ]
  • symbol: NWMN_RS03385
  • product: cation:proton antiporter
  • replicon: chromosome
  • strand: +
  • coordinates: 672494..674896
  • length: 2403
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (672494..674896) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0593

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTTTGGTTTATTTATTAATTGCTATACTTGTGATTATGGCGATGATACTTCTAATG
    TCTAAACGTAGAGCATTGGCTAAATATGCCGGGTACATAGCGTTGGTTGCACCTGTAATT
    TCATCTATCTATTTTTTGATTCAAATACCATCAGTAGCTAAACTGCAATATCTTTCTACC
    TCTATTCCATGGATTAAGACATTAGATATTAATTTAGATTTACGTTTAGATGGTTTAAGT
    TTAATGTTTTCTCTTATTATTTCACTTATTGGAATTGCAGTATTCTTCTATGCAACTCAA
    TATTTATCCTCTCGAAAAGACAATTTACCAAGGTTTTATTTTTATTTAACGTTATTTATG
    TTCAGTATGATTGGTATTGTATTATCAGACAATACGATATTGATGTACATTTTTTGGGAA
    TTAACGAGTGTATCATCATTTTTATTGATTTCATATTGGTATAACAATGGTGACAGTCAA
    TTTGGTGCGATTCAATCATTTATGATTACAGTATTTGGTGGATTGGCGTTATTAGTTGGT
    TTTATTATGCTGTATATCATGACAGGAACGAATAACATCACAGAGATATTAGGACAAGCA
    GATCATATTAAGAATCATGGATTGTTTATCCCTATGATTTTTATGTTTTTATTAGGTGCA
    TTTACAAAATCAGCACAATTTCCATTTCATATTTGGCTACCTAGAGCAATGGCTGCACCT
    ACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTA
    CTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTT
    GGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGT
    ATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGT
    GGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTT
    GGTGCGCTATTTCATCTAATGAATCATGCCATCTTTAAATGTGCGCTTTTCATGGGAGTA
    GGTATTTTAGATCATGAAGCAGGTTCAAGGGATATACGAATTTTAAGTGGAATGCGTCAA
    CTATTTCCTAAAATGAATCTAGTCATGACGATAGCGGCTCTATCTATGGCTGGAGTACCA
    TTTTTAAATGGATTTTTAAGTAAAGAAATGTTTTTAGATGCATTAACACAAACTGGACAA
    TTATCCCAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGT
    GTTTTTACATTCACATATGCACTATACATGGTAAAAGAAGTATTTTGGACAAAATATGAT
    TCTAAGGTTTTTACTAAAAAAAATATCCACGAACCATGGTTGTTTAGTTTACCATCTCTT
    ATATTAATGGTGCTAGTACCTGTAATCTTTTTTGTACCAAATATATTTGGGAAGGGGATT
    ATCGTTCTAGCATTAAGAGCTGTATCAGGTGGTAATCATCAAATTGATCAATTGGCACCA
    CATGTTTCGCAATGGCATGGATTTAACATACCGCTTCTTTTAACCATCATCATTATTTTA
    TTGGGTAGTGTACTAGCAATCAAAGTAGATTGGAAAAAAGTGTTCACAGGTAAAATTAGA
    CAGATTTCAGTTTCAAAAAGCTATGAGATGGTATATCGACATTTTGAAAAGTTTGCTACG
    AAGCGATTTAAACGTGTTATGCAAGATCGTTTAAACCAATACATTATTATGACCTTAGGC
    ATATTTATGATTATCATTGGATATGGTTATATTCGAATTGGACTTCCTAAAGTACATCAG
    TTACATGTTTCTGAATTTGGGGCATTAGAAATTATATTAGCAATCGTAACTGTCACAATT
    GGTATTTCTTTAATTTTTATACGTCAACGACTGACAATGGTCATTTTAAATGGAGTCATC
    GGATTTGTTGTGACCTTATTCTTTATAGCAATGAAAGCCCCTGATCTAGCATTGACTCAG
    CTAGTAGTTGAAACAATAACGACGATACTATTTATTGTCAGTTTTTCAAGATTACCAAAC
    GTGCCAAGATCTAACGCTAACAAAAAAAGAGAAATAATTAAAATTTCTGTATCACTCTTG
    ATGGCACTTATTGTTGTATCATTAATTTTTATTACACAACAAACAGATGGTTTATCATCA
    ATATCAGACTTTTATTTAAAAGCTGACAAACTAACAGGTGGTAAAAATATTGTAAATGCG
    ATACTTGGTGACTTTAGAGCATTAGATACATTATTTGAAGGATTAGTGTTAATTATTACT
    GGGCTAGGTATTTACACATTATTAAATTATCAAGATCGGAGGGGACAAGATGAAAGAGAA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2403

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS03385 [old locus tag: NWMN_0593 ]
  • symbol: NWMN_RS03385
  • description: cation:proton antiporter
  • length: 800
  • theoretical pI: 10.0697
  • theoretical MW: 89686
  • GRAVY: 0.795125

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 738.8)
    and 5 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 314.6)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 181.7)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 146.1)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 141.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 104.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 223.5)
    and 3 more
    no clan defined DUF4040; Domain of unknown function (DUF4040) (PF13244; HMM-score: 67.6)
    Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 41.3)
    TspO_MBR; TspO/MBR family (PF03073; HMM-score: 17.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 21
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.216295
    • TAT(Tat/SPI): 0.018
    • LIPO(Sec/SPII): 0.007659
  • predicted transmembrane helices (TMHMM): 21

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIYFLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYLSSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQFGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLLGAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYIVTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASIYVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIAALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYMVKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAVSGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSKSYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVSEFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLVVETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSSISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDERE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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