PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• pan locus tag^?: SAUPAN002493000
• symbol: SAUSA300_0610
• pan gene symbol^?: mnhA2
• synonym:
• product: putative monovalent cation/H+ antiporter subunit A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• symbol: SAUSA300_0610
• product: putative monovalent cation/H+ antiporter subunit A
• replicon: chromosome
• strand: +
• coordinates: 682134..684536
• length: 2403
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912835 NCBI
• RefSeq: YP_493313 NCBI
• BioCyc: GH3C-605 BioCyc
• MicrobesOnline: 1292125 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  
  ATGAGTTTGGTTTATTTATTAATTGCTATACTTGTGATTATGGCGATGATACTTCTAATG
  TCTAAACGTAGAGCATTGGCTAAATATGCCGGGTACATAGCGTTGGTTGCACCTGTAATT
  TCATCTATCTATTTTTTGATTCAAATACCATCAGTAGCTAAACTGCAATATCTTTCTACC
  TCTATTCCATGGATTAAGACATTAGATATTAATTTAGATTTACGTTTAGATGGTTTAAGT
  TTAATGTTTTCTCTTATTATTTCACTTATTGGAATTGCAGTATTCTTCTATGCAACTCAA
  TATTTATCCTCTCGAAAAGACAATTTACCAAGGTTTTATTTTTATTTAACGTTATTTATG
  TTCAGTATGATTGGTATTGTATTATCAGACAATACGATATTGATGTACATTTTTTGGGAA
  TTAACGAGTGTATCATCATTTTTATTGATTTCATATTGGTATAACAATGGTGACAGTCAA
  TTTGGTGCGATTCAATCATTTATGATTACAGTATTTGGTGGATTGGCGTTATTAGTTGGT
  TTTATTATGCTGTATATCATGACAGGAACGAATAACATCACAGAGATATTAGGACAAGCA
  GATCATATTAAGAATCATGGATTGTTTATCCCTATGATTTTTATGTTTTTATTAGGTGCA
  TTTACAAAATCAGCACAATTTCCATTTCATATTTGGCTACCTAGAGCAATGGCTGCACCT
  ACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTA
  CTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTT
  GGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGT
  ATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGT
  GGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTT
  GGTGCGCTATTTCATCTAATGAATCATGCCATCTTTAAATGTGCGCTTTTCATGGGAGTA
  GGTATTTTAGATCATGAAGCAGGTTCAAGGGATATACGAATTTTAAGTGGAATGCGTCAA
  CTATTTCCTAAAATGAATCTAGTCATGACGATAGCGGCTCTATCTATGGCTGGAGTACCA
  TTTTTAAATGGATTTTTAAGTAAAGAAATGTTTTTAGATGCATTAACACAAACTGGACAA
  TTATCCCAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGT
  GTTTTTACATTCACATATGCACTATACATGGTAAAAGAAGTATTTTGGACAAAATATGAT
  TCTAAGGTTTTTACTAAAAAAAATATCCACGAACCATGGTTGTTTAGTTTACCATCTCTT
  ATATTAATGGTGCTAGTACCTGTAATCTTTTTTGTACCAAATATATTTGGGAAGGGGATT
  ATCGTTCTAGCATTAAGAGCTGTATCAGGTGGTAATCATCAAATTGATCAATTGGCACCA
  CATGTTTCGCAATGGCATGGATTTAACATACCGCTTCTTTTAACCATCATCATTATTTTA
  TTGGGTAGTGTACTAGCAATCAAAGTAGATTGGAAAAAAGTGTTCACAGGTAAAATTAGA
  CAGATTTCAGTTTCAAAAAGCTATGAGATGGTATATCGACATTTTGAAAAGTTTGCTACG
  AAGCGATTTAAACGTGTTATGCAAGATCGTTTAAACCAATACATTATTATGACCTTAGGC
  ATATTTATGATTATCATTGGATATGGTTATATTCGAATTGGACTTCCTAAAGTACATCAG
  TTACATGTTTCTGAATTTGGGGCATTAGAAATTATATTAGCAATCGTAACTGTCACAATT
  GGTATTTCTTTAATTTTTATACGTCAACGACTGACAATGGTCATTTTAAATGGAGTCATC
  GGATTTGTTGTGACCTTATTCTTTATAGCAATGAAAGCCCCTGATCTAGCATTGACTCAG
  CTAGTAGTTGAAACAATAACGACGATACTATTTATTGTCAGTTTTTCAAGATTACCAAAC
  GTGCCAAGATCTAACGCTAACAAAAAAAGAGAAATAATTAAAATTTCTGTATCACTCTTG
  ATGGCACTTATTGTTGTATCATTAATTTTTATTACACAACAAACAGATGGTTTATCATCA
  ATATCAGACTTTTATTTAAAAGCTGACAAACTAACAGGTGGTAAAAATATTGTAAATGCG
  ATACTTGGTGACTTTAGAGCATTAGATACATTATTTGAAGGATTAGTGTTAATTATTACT
  GGGCTAGGTATTTACACATTATTAAATTATCAAGATCGGAGGGGACAAGATGAAAGAGAA
  TGA
  60
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  1140
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  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2403
  

⊟Protein

⊟General

• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• symbol: SAUSA300_0610
• description: putative monovalent cation/H+ antiporter subunit A
• length: 800
• theoretical pI: 10.0697
• theoretical MW: 89686
• GRAVY: 0.795125

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 738.8)
  and 5 more
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 314.6)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 181.7)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 146.1)
  NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 141.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 104.7)
• TheSEED  :
  • Na(+) H(+) antiporter subunit A (TC 2.A.63.1.3)
  Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit A (TC 2.A.63.1.3)
• PFAM:
  ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 223.5)
  and 3 more
  no clan defined DUF4040; Domain of unknown function (DUF4040) (PF13244; HMM-score: 67.6)
  Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 41.3)
  TspO_MBR; TspO/MBR family (PF03073; HMM-score: 17.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 21
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.216295
  • TAT(Tat/SPI): 0.018
  • LIPO(Sec/SPII): 0.007659
• predicted transmembrane helices (TMHMM): 21

⊟Accession numbers

• GI: 87160720 NCBI
• RefSeq: YP_493313 NCBI
• UniProt: Q2FJ15 UniProt

⊟Protein sequence

• MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIYFLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYLSSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQFGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLLGAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYIVTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASIYVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIAALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYMVKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAVSGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSKSYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVSEFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLVVETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSSISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDERE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0609 > SAUSA300_0610 > SAUSA300_0611 > SAUSA300_0612 > SAUSA300_0613 > SAUSA300_0614 > SAUSA300_0615 > SAUSA300_0616

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications