PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• pan locus tag^?: SAUPAN002493000
• symbol: SAUSA300_0610
• pan gene symbol^?: mnhA2
• synonym:
• product: putative monovalent cation/H+ antiporter subunit A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• symbol: SAUSA300_0610
• product: putative monovalent cation/H+ antiporter subunit A
• replicon: chromosome
• strand: +
• coordinates: 682134..684536
• length: 2403
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912835 NCBI
• RefSeq: YP_493313 NCBI
• BioCyc: see SAUSA300_RS03275
• MicrobesOnline: 1292125 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  
  ATGAGTTTGGTTTATTTATTAATTGCTATACTTGTGATTATGGCGATGATACTTCTAATG
  TCTAAACGTAGAGCATTGGCTAAATATGCCGGGTACATAGCGTTGGTTGCACCTGTAATT
  TCATCTATCTATTTTTTGATTCAAATACCATCAGTAGCTAAACTGCAATATCTTTCTACC
  TCTATTCCATGGATTAAGACATTAGATATTAATTTAGATTTACGTTTAGATGGTTTAAGT
  TTAATGTTTTCTCTTATTATTTCACTTATTGGAATTGCAGTATTCTTCTATGCAACTCAA
  TATTTATCCTCTCGAAAAGACAATTTACCAAGGTTTTATTTTTATTTAACGTTATTTATG
  TTCAGTATGATTGGTATTGTATTATCAGACAATACGATATTGATGTACATTTTTTGGGAA
  TTAACGAGTGTATCATCATTTTTATTGATTTCATATTGGTATAACAATGGTGACAGTCAA
  TTTGGTGCGATTCAATCATTTATGATTACAGTATTTGGTGGATTGGCGTTATTAGTTGGT
  TTTATTATGCTGTATATCATGACAGGAACGAATAACATCACAGAGATATTAGGACAAGCA
  GATCATATTAAGAATCATGGATTGTTTATCCCTATGATTTTTATGTTTTTATTAGGTGCA
  TTTACAAAATCAGCACAATTTCCATTTCATATTTGGCTACCTAGAGCAATGGCTGCACCT
  ACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTA
  CTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTT
  GGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGT
  ATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGT
  GGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTT
  GGTGCGCTATTTCATCTAATGAATCATGCCATCTTTAAATGTGCGCTTTTCATGGGAGTA
  GGTATTTTAGATCATGAAGCAGGTTCAAGGGATATACGAATTTTAAGTGGAATGCGTCAA
  CTATTTCCTAAAATGAATCTAGTCATGACGATAGCGGCTCTATCTATGGCTGGAGTACCA
  TTTTTAAATGGATTTTTAAGTAAAGAAATGTTTTTAGATGCATTAACACAAACTGGACAA
  TTATCCCAATTTAGTTTGATTTCAATGATAGCTATCGTGTTTGTTGGTGTTATTGCGAGT
  GTTTTTACATTCACATATGCACTATACATGGTAAAAGAAGTATTTTGGACAAAATATGAT
  TCTAAGGTTTTTACTAAAAAAAATATCCACGAACCATGGTTGTTTAGTTTACCATCTCTT
  ATATTAATGGTGCTAGTACCTGTAATCTTTTTTGTACCAAATATATTTGGGAAGGGGATT
  ATCGTTCTAGCATTAAGAGCTGTATCAGGTGGTAATCATCAAATTGATCAATTGGCACCA
  CATGTTTCGCAATGGCATGGATTTAACATACCGCTTCTTTTAACCATCATCATTATTTTA
  TTGGGTAGTGTACTAGCAATCAAAGTAGATTGGAAAAAAGTGTTCACAGGTAAAATTAGA
  CAGATTTCAGTTTCAAAAAGCTATGAGATGGTATATCGACATTTTGAAAAGTTTGCTACG
  AAGCGATTTAAACGTGTTATGCAAGATCGTTTAAACCAATACATTATTATGACCTTAGGC
  ATATTTATGATTATCATTGGATATGGTTATATTCGAATTGGACTTCCTAAAGTACATCAG
  TTACATGTTTCTGAATTTGGGGCATTAGAAATTATATTAGCAATCGTAACTGTCACAATT
  GGTATTTCTTTAATTTTTATACGTCAACGACTGACAATGGTCATTTTAAATGGAGTCATC
  GGATTTGTTGTGACCTTATTCTTTATAGCAATGAAAGCCCCTGATCTAGCATTGACTCAG
  CTAGTAGTTGAAACAATAACGACGATACTATTTATTGTCAGTTTTTCAAGATTACCAAAC
  GTGCCAAGATCTAACGCTAACAAAAAAAGAGAAATAATTAAAATTTCTGTATCACTCTTG
  ATGGCACTTATTGTTGTATCATTAATTTTTATTACACAACAAACAGATGGTTTATCATCA
  ATATCAGACTTTTATTTAAAAGCTGACAAACTAACAGGTGGTAAAAATATTGTAAATGCG
  ATACTTGGTGACTTTAGAGCATTAGATACATTATTTGAAGGATTAGTGTTAATTATTACT
  GGGCTAGGTATTTACACATTATTAAATTATCAAGATCGGAGGGGACAAGATGAAAGAGAA
  TGA
  60
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  1140
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  1260
  1320
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  1440
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  1560
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  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2403
  

⊟Protein

⊟General

• locus tag: SAUSA300_0610 [new locus tag: SAUSA300_RS03275 ]
• symbol: SAUSA300_0610
• description: putative monovalent cation/H+ antiporter subunit A
• length: 800
• theoretical pI: 10.0697
• theoretical MW: 89686
• GRAVY: 0.795125

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 738.8)
  and 5 more
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 314.6)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 181.7)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 146.1)
  NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 141.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 104.7)
• TheSEED  :
  • Na(+) H(+) antiporter subunit A (TC 2.A.63.1.3)
  Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit A (TC 2.A.63.1.3)
• PFAM:
  ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 223.5)
  and 3 more
  no clan defined DUF4040; Domain of unknown function (DUF4040) (PF13244; HMM-score: 67.6)
  Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 41.3)
  TspO_MBR; TspO/MBR family (PF03073; HMM-score: 17.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 21
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.216295
  • TAT(Tat/SPI): 0.018
  • LIPO(Sec/SPII): 0.007659
• predicted transmembrane helices (TMHMM): 21

⊟Accession numbers

• GI: 87160720 NCBI
• RefSeq: YP_493313 NCBI
• UniProt: Q2FJ15 UniProt

⊟Protein sequence

• MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIYFLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYLSSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQFGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLLGAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYIVTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASIYVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIAALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYMVKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAVSGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSKSYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVSEFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLVVETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSSISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDERE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0609 > SAUSA300_0610 > SAUSA300_0611 > SAUSA300_0612 > SAUSA300_0613 > SAUSA300_0614 > SAUSA300_0615 > SAUSA300_0616

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications