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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0611 [new locus tag: SAUSA300_RS03280 ]
- pan locus tag?: SAUPAN002495000
- symbol: SAUSA300_0611
- pan gene symbol?: mnhB2
- synonym:
- product: putative monovalent cation/H+ antiporter subunit B
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0611 [new locus tag: SAUSA300_RS03280 ]
- symbol: SAUSA300_0611
- product: putative monovalent cation/H+ antiporter subunit B
- replicon: chromosome
- strand: +
- coordinates: 684523..684948
- length: 426
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914424 NCBI
- RefSeq: YP_493314 NCBI
- BioCyc: see SAUSA300_RS03280
- MicrobesOnline: 1292126 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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421ATGAAAGAGAATGATGTCGTGTTAAGAACGGTCACGAAACTTGTTGTATTTATTTTATTG
ACTTTCGGATTCTATGTCTTCTTCGCAGGTCATAATAATCCTGGTGGTGGGTTTATTGGT
GGTTTAATATTTAGTTCAGCGTTTATTTTAATGTTTCTGGCTTTTAATGTTGAAGAGGTT
TTAGAAAGTTTACCGATTGATTTTAGAATTTTAATGATTATTGGAGCATTGGTATCATCT
ATTACTGCGATAATACCTATGTTTTTTGGAAAACCATTTTTGTCTCAATATGAAACAACT
TGGATACTTCCAATTTTAGGACAAATTCATGTAAGTACAATAACACTTTTTGAATTAGGT
ATTTTATTCTCAGTTGTTGGTGTTATTGTCACAGTGATGTTGTCGCTTAGCGGAGGTCGA
TCATGA60
120
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426
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0611 [new locus tag: SAUSA300_RS03280 ]
- symbol: SAUSA300_0611
- description: putative monovalent cation/H+ antiporter subunit B
- length: 141
- theoretical pI: 5.6084
- theoretical MW: 15454.4
- GRAVY: 1.15035
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00943; HMM-score: 103.6)
- TheSEED :
- Na(+) H(+) antiporter subunit B (TC 2.A.63.1.3)
- PFAM: no clan defined MnhB; Domain related to MnhB subunit of Na+/H+ antiporter (PF04039; HMM-score: 110.8)and 1 moreDUF3493; Protein of unknown function (DUF3493) (PF11998; HMM-score: 7.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004138
- TAT(Tat/SPI): 0.000248
- LIPO(Sec/SPII): 0.008493
- predicted transmembrane helices (TMHMM): 4
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIFSSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWILPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.