From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04105 [old locus tag: NWMN_0725 ]
  • pan locus tag?: SAUPAN002670000
  • symbol: NWMN_RS04105
  • pan gene symbol?: —
  • synonym:
  • product: hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS04105 [old locus tag: NWMN_0725 ]
  • symbol: NWMN_RS04105
  • product: hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 813283..813933
  • length: 651
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (813283..813933) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0725

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGTGTACATCAATATTTTAAAAGATTATCAGATATGGAAAGACTTATAAGATTACCT
    GGAAAATTTAAATATTTTGAACACAATGTTGCAGCACACTCCTTTAAAGTAACTAAAATT
    GCACAATATCTAGCAACAGTTGAAGAATATCATGGACGAAAGATTAATTGGAAAAGCTTA
    TATGAAAAAGCATTAAATCATGATTTCGCCGAAGTGTTTACTGGTGATATAAAAACACCT
    GTTAAATATGCGAGTAGTGAATTAAAAAAATTATTTTCGCAAGTTGAAGAAGAAATGGTA
    GAGACCTTTATTGAAGAAGAAATTCCATTACAATATAGAGATGTTTATAAGCAACGACTG
    CAAGAAGGTAAAGATGATTCATTAGAAGGCCAAATACTTTCAGTTGCTGATAAAATTGAT
    TTGCTTTATGAAACATTTGGAGAAATACAAAAACGTAATCCCGAAGAATTATTTTTCGAA
    ATTTATGAAATGAGTCTAGAAACAATTATTCAATTTGACCATTTAGCATCTGTACAAGAT
    TTTATTAATAATATCATTCCAGAAATGTTGACTGAAAACTTTATACCTAGAACAGAATTA
    AGAGAAACAACCATGAACATTTTAAATAAAAGAAAAGAGGAAAATGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    651

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS04105 [old locus tag: NWMN_0725 ]
  • symbol: NWMN_RS04105
  • description: hydrolase
  • length: 216
  • theoretical pI: 4.80408
  • theoretical MW: 25704
  • GRAVY: -0.559722

⊟Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 16)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HD_PDEase (CL0237) HD_2; HD containing hydrolase-like enzyme (PF12917; HMM-score: 280)
    and 2 more
    HD_3; HD domain (PF13023; HMM-score: 34.1)
    HD; HD domain (PF01966; HMM-score: 19.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002732
    • TAT(Tat/SPI): 0.00025
    • LIPO(Sec/SPII): 0.000669
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYLATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS04105&oldid=61064"