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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04720 [old locus tag: NWMN_0837 ]
  • pan locus tag?: SAUPAN003076000
  • symbol: NWMN_RS04720
  • pan gene symbol?: addB
  • synonym:
  • product: ATP-dependent helicase/deoxyribonuclease subunit B

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS04720 [old locus tag: NWMN_0837 ]
  • symbol: NWMN_RS04720
  • product: ATP-dependent helicase/deoxyribonuclease subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 925155..928631
  • length: 3477
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (925155..928631) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0837

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACATTACATGCTTATTTAGGTAGAGCGGGAACAGGTAAGTCTACGAAAATGTTGACC
    GAAATAAAACAAAAAATGAAAGCAGATCCGCTTGGAGATCCAATCATTTTAATTGCGCCA
    ACTCAAAGTACATTTCAATTAGAACAAGCCTTTGTCAATGATCCGGAATTAAATGGTAGT
    TTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGT
    AGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTT
    CAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGT
    GAAAAATTAACAGAACAAATTCAAGATTTTAAAAAATATGCAGTAACGCCTGAACATTTA
    GAACACTTTATTGCTGATAAAAATATGCAAACTCGAACTAAAAATAAGTTAGAGGATATT
    GCTTTAATATACCGTGAGTTCGAACAACGCATTCAAAACGAGTTTATTACGGGTGAGGAT
    TCATTACAATATTTTATTGATTGTATGCCGAAATCAGAGTGGCTAAAACGTGCTGATATA
    TATATTGATGGTTTTCACAACTTTTCAACGATTGAGTATTTAATAATCAAAGGATTAATT
    AAATATGCGAAGAGTGTCACAATTATATTGACGACAGATGGTAACCACGATCAATTTAGT
    TTATTTAGAAAACCATCGGAAGTGTTACGACATATTGAAGAAATAGCAAATGAACTCAAT
    ATTTCTATTGAACGTCAATATTTCAACCAATTATATCGCTTCAATAATCAAGATTTAAAG
    CATCTTGAACAAGAATTTGATGCACTTCAAATCAATCGAGTGGCATGTCAAGGTCATATC
    AATATTTTAGAATCTGCGACTATGAGAGAGGAAATAAATGAAATTGCGCGACGTATCATC
    GTTGATATTCGTGATAAGCAATTACGATATCAAGATATTGCTATTTTATATCGTGATGAA
    TCTTATGCTTATTTATTTGATTCCATATTACCGCTTTATAATATTCCTTATAATATTGAT
    ACAAAGCGTTCGATGACACATCATCCGGTCATGGAAATGATTCGTTCATTGATTGAAGTT
    ATTCAATCTAATTGGCAAGTGAATCCAATGCTACGCTTATTGAAGACTGATGTGTTAACG
    GCATCATATCTAAAAAGTGCATACTTAGTTGATTTACTTGAAAATTTTGTACTTGAACGT
    GGTATATACGGTAAACGTTGGTTAGATGATGAGCTATTTAATGTCGAACATTTTAGCAAA
    ATGGGGCGTAAAGGGCATAAACTGACCGAAGATGAACGTAACACATTTGAACAAGTCGTT
    AAGTTAAAGAAAGATGTCATTGATAAAATTTTACATTTTGAAAAGCAAATGTCACAAGCG
    GAAACTGTAAAAGATTTTGCAACTGCTTTTTATGAAAGTATGGAATATTTCGAACTGCCA
    AATCAATTGATGACAGAGCGAGATGAACTTGATTTAAATGGTAATCATGAAAAGGCGGAG
    GAAATTGATCAAATATGGAATGGCTTAATTCAAATCCTTGATGACTTAGTTCTAGTATTT
    GGAGATGAACCAATGTCGATGGAACGTTTCTTAGAAGTATTTGATATTGGTTTAGAACAA
    TTAGAATTTGTTATGATTCCGCAAACATTGGACCAAGTAAGTATTGGTACGATGGATTTG
    GCTAAAGTCGATAATAAGCAACATGTTTACTTAGTAGGTATGAATGATGGAACGATGCCA
    CAACCAGTAACTGCGTCAAGCTTGATTACAGATGAAGAAAAGAAATACTTTGAACAGCAG
    GCTAATGTCGAGTTAAGTCCAACATCAGATATTTTACAGATGGATGAAGCATTTGTTTGT
    TATGTTGCTATGACTAGAGCTAAGGGAGATGTTACATTTTCTTACAGTCTAATGGGATCA
    AGTGGTGATGATAAGGAGATCAGCCCATTTTTAAATCAAATTCAATCATTGTTCAACCAA
    TTGGAAATTACTAACATTCCTCAATACCATGAAGTTAACCCATTGTCACTAATGCAACAT
    GCTAAGCAAACCAAAATTACATTATTTGAAGCATTGCGTGCTTGGTTATATGATGAAATT
    GTGGCTGATAGTTGGTTAGATGCTTATCAAGTAATTAGAGATAGCGATCATTTAAATCAA
    GGTTTAGATTATTTAATGTCAGCATTAACGTTTGACAATGAAACTGTAAAATTAGGTGAA
    ACGTTGTCTAAAGATTTATATGGTAAGGAAATCAATGCCAGTGTATCCCGTTTTGAAGGT
    TATCAACAATGCCCATTTAAACACTATGCGTCACATGGTCTGAAACTAAATGAGCGAACG
    AAGTATGAACTTCAAAACTTTGATTTAGGTGATATTTTCCATTCTGTTTTAAAATATATA
    TCTGAACGTATTAATGGCGATTTTAAACAATTAGACCTGAAAAAAATAAGACAATTAACG
    AATGAAGCATTGGAAGAAATTTTACCTAAAGTTCAGTTTAATTTATTAAATTCTTCAGCT
    TACTATCGTTATTTATCAAGACGCATTGGCGCTATTGTAGAAACAACACTAAGCGCATTA
    AAATATCAAGGCACGTATTCAAAGTTTATGCCAAAACATTTTGAGACAAGTTTTAGAAGG
    AAACCAAGAACAAATGACGAATTAATTGCACAAACATTAACGACAACTCAAGGTATTCCA
    ATTAATATTAGAGGGCAAATTGACCGTATCGATACGTATACAAAGAATGATACAAGTTTT
    GTTAATATCATTGACTATAAATCCTCTGAAGGTAGTGCGACACTTGATTTAACGAAAGTA
    TATTATGGTATGCAAATGCAAATGATGACATACATGGATATCGTTTTACAAAATAAACAA
    CGCCTTGGATTAACAGATATTGTGAAACCAGGTGGATTATTATACTTCCATGTACATGAA
    CCTAGAATTAAATTTAAATCATGGTCTGATATTGATGAAGATAAACTAGAACAAGATTTA
    ATTAAAAAGTTTAAGTTGAGTGGTTTAGTTAATGCAGACCAAACTGTTATTGATGCATTG
    GATATTCGTTTAGAACCTAAATTCACTTCAGATATTGTACCAGTTGGTTTGAATAAAGAT
    GGCTCTTTGAGTAAACGAGGCAGCCAAGTGGCAGATGAAGCAACGATTTATAAATTCATC
    CAACATAACAAAGAGAATTTTATAGAAACAGCTTCAAATATTATGGATGGACATACTGAA
    GTTGCACCATTAAAGTACAAACAAAAATTGCCATGTGCTTTTTGTAGTTATCAATCGGTA
    TGTCATGTAGATGGCATGATTGATAGTAAGCGATATCGAACTGTAGATGAAACAATAAAT
    CCAATTGAAGCAATTCAAAATATTAACATTAATGATGAATTTGGGGGTGAGCAATAG
    60
    120
    180
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    300
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    480
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    720
    780
    840
    900
    960
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    2160
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    2700
    2760
    2820
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    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3477

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS04720 [old locus tag: NWMN_0837 ]
  • symbol: NWMN_RS04720
  • description: ATP-dependent helicase/deoxyribonuclease subunit B
  • length: 1158
  • theoretical pI: 5.06875
  • theoretical MW: 134490
  • GRAVY: -0.423575

Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddB subunit (TIGR02773; EC 3.1.-.-,3.6.4.12; HMM-score: 1417.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent nuclease subunit B (TIGR02774; HMM-score: 173.6)
    probable DNA repair protein (TIGR03623; HMM-score: 32.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 30.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair double-strand break repair protein AddB (TIGR02786; HMM-score: 30)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 14.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 174.4)
    and 3 more
    P-loop_NTPase (CL0023) UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 58.6)
    AAA_30; AAA domain (PF13604; HMM-score: 14.5)
    no clan defined Imm21; Immunity protein 21 (PF15589; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, [4Fe-4S] cluster
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002995
    • TAT(Tat/SPI): 0.000315
    • LIPO(Sec/SPII): 0.00049
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQSTFQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQEQQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDIALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKGLIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNNQDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIAILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLRLLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKGHKLTEDERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERDELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMIPQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVELSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLEITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQGLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNERTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLLNSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLTTTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYMDIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLVNADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFIETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQNININDEFGGEQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]