PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS04840 [old locus tag: NWMN_0861 ]
• pan locus tag^?: SAUPAN003146000
• symbol: NWMN_RS04840
• pan gene symbol^?: opp4A
• synonym:
• product: ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS04840 [old locus tag: NWMN_0861 ]
• symbol: NWMN_RS04840
• product: ABC transporter substrate-binding protein
• replicon: chromosome
• strand: +
• coordinates: 956707..958422
• length: 1716
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (956707..958422) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0861

⊟Phenotype

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⊟DNA sequence

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  ATGGGGAAGCTAATTAAATATATTTCAATACTTCTTATTGTCGTTTTAGTGTTGAGTGCT
  TGCGGAAAAAGCAGTAATAAAGATGAAGGAGTAAAAGATGCTACTAAAACGGAAACCTCA
  AAACATAAAGGTGGTACCTTAAATGTAGCATTAACAGCACCGCCAAGTGGTGTTTATTCT
  TCGTTATTAAATAGTACACATGCAGATTCTGTAGTTGAGGGATATTTTAACGAAAGCTTA
  TTAGCAACTGATAAAAAAATACGTCCTAAGGCATATATTGCTTCATGGAAGGACATCGAG
  CCGGCTAAGAAAATAGAATTTAAAATTAAAAAAGGTATTAAATGGCATGATGGTAATGAA
  TTGAAAATTGATGATTGGATTTATTCAATTGAAGTCTTAGCTAACAAGGACTACGAAGGT
  GCTTATTATCCAAGTGTAGAAAATATCCAAGGTGCGAAAGATTATCATGAAGGAAAAACT
  GATCATATTAGCGGATTGAAGAAAATAGATGACTACACTATGCAGGTTACATTTGATAAA
  AAACAAGAAAATTACTTAACAGGATTTATTACTGGACCTTTATTAAGTAAAAAATATTTA
  TCAGATGTACCAATTAAAGATTTAGCGAAATCAGATAAAATCCGAAAATATCCTATTGGT
  ATTGGACCGTATAAAGTTAAGAAAATCGTTCCAGGTGAGGCTGTTCAACTCGTTAAATTT
  GATGATTATTGGCAAGGTAAGCCTGCACTAGACAAAATCAATTTAAAAGTTATTGATCAA
  GCGCAAATTATTAAGGCAATGGAAAAAGGCGATATTGATGTTGCGAATGATGCTACCGGT
  GCAATGGCAAAAGATGCTAAGTCATCTAATGCTGGTCTCAAGGTATTATCTGCGCCAAGC
  TTAGACTACGGTTTAATAGGATTCGTATCTCATGATTACGATAAAAAAGCTAATAAAACT
  GGTAAAGTGAGACCAAAATATGAAGACAAAGAATTACGTAAAGCAATGCTTTATGCAATT
  GATAGAGAAAAATGGATCAAAGCGTTTTTCAATGGTTACGCTAGTGAAATCAATAGTTTT
  GTACCATCTATGCATTGGATAGCAGCCAATCCTAAGGACCTAAATGATTACAAATATGAT
  CCTGAAAAAGCTAAAAAAATCTTAGATAAGTTAGGTTATAAAGATAGAGATGGTGACGGA
  TTTAGAGAAGATCCTAAAGGTAATAAATTTGAGATTAACTTTAAACATTATTCAGGTTCT
  AATCCTACTTTTGAACCAAGAACTGCTGCGATAAAAGATTTCTGGGAAAAAGTTGGCTTG
  AAAACAAATGTGAAGTTAGTAGAATTCGGTAAATATAATGAAGACTTAGCAAATGCATCT
  AAAGATATGGAAGTGTACTTCAGATCATGGGCAGGAGGTACAGATCCAGATCCATCAGAT
  TTATACCACACTGATAGACCTCAAAATGAAATGAGAACAGTTTTACCAAAATCAGATCAA
  TATTTAGATGATGCATTAGACTTCGAAAAAGTAGGCATTGATGAAAAGAAACGTAAAGAT
  ATTTATGTTAAATGGCAAAAATATATGAATGATGAGTTACCTGGATTACCAATGTTCCAA
  GGTAAATCGATAACTATTGTTAACGATAAAGTACGAAACTTAGACATTGAAATTGGAACT
  GATCAAAGTTTATATAATTTAACTAAAGAAGCTTAG
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⊟Protein

⊟General

• locus tag: NWMN_RS04840 [old locus tag: NWMN_0861 ]
• symbol: NWMN_RS04840
• description: ABC transporter substrate-binding protein
• length: 571
• theoretical pI: 9.0042
• theoretical MW: 64576.1
• GRAVY: -0.636602

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 94.5)
  Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 78.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 265.9)
  and 1 more
  GBD (CL0202) P_proprotein; Proprotein convertase P-domain (PF01483; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.53
  • Cellwall Score: 8.76
  • Extracellular Score: 0.7
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.000481
  • TAT(Tat/SPI): 0.000083
  • LIPO(Sec/SPII): 0.99921
  • Cleavage Site: CS pos: 20-21. LSA-CG. Pr: 0.9997
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446439332 NCBI
• RefSeq: WP_000517187 NCBI
• UniProt: see NWMN_0861

⊟Protein sequence

• MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTDHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see NWMN_0861

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications