PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
• pan locus tag^?: SAUPAN001648000
• symbol: NWMN_RS05650
• pan gene symbol^?: —
• synonym:
• product: DNA replication protein DnaC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
• symbol: NWMN_RS05650
• product: DNA replication protein DnaC
• replicon: chromosome
• strand: +
• coordinates: 1109400..1110179
• length: 780
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1109400..1110179) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1004

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAACCACTATTCAGCGAAAAGATAAACGAAAGCTTGAAAAAATATCAACCTACTCAT
  GTCGAAAAAGGATTGAAATGTGAGAGATGTGGAAGTGAATACGACTTATATAAGTTTGCT
  CCTACTAAAAAACACCCGAATGGTTACGAGTATAAAGACGGTTGCAAATGTGAAATCTAT
  GAGGAATATAAGCGAAACAAGCAACGGAAGATAAACAACATATTCAATCAATCAAACGTT
  AATCCGTCTTTAAGAGATGCAACAGTCAAAAACTACAAGCCACAAAATGAAAAACAAGTA
  CACGCTAAACAAACAGCAATAGAGTACGTACAAGGCTTCTCTACAAAAGAACCAAAATCA
  TTAATATTGCAAGGTTCATACGGAACTGGTAAAAGCCACCTAGCATACGCTATCGCAAAA
  GCAGTCAAAGCTAAAGGGCATACGGTTGCTTTTATGCACATACCAATGTTGATGGATCGT
  ATCAAAGCGACATACAACAAAAATGCAGTAGAGACTACAGACGAGCTAGTCAGATTGCTA
  AGTGATATTGATTTACTTGTACTAGATGATATGGGTGTAGAAAACACAGAGCACACTTTA
  AATAAACTTTTCAGCATTGTTGATAACAGAGTAGGTAAAAACAACATCTTTACAACTAAC
  TTTAGTGATAAAGAACTAAATCAAAATATGAACTGGCAACGTATCAATTCAAGAATGAAA
  CACAATGCGAGAAAAGTAAGAGTAATCGGAGACGATTTCAGGGAGCGAGATGCATGGTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
• symbol: NWMN_RS05650
• description: DNA replication protein DnaC
• length: 259
• theoretical pI: 9.83133
• theoretical MW: 30058.1
• GRAVY: -0.847876

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 41.5)
  and 7 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 30.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 14)
  Genetic information processing Protein synthesis tRNA and rRNA base modification sulfur relay protein TusD/DsrE (TIGR03012; HMM-score: 13.8)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 13.5)
  Cellular processes Cellular processes Cell division ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 13)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 13)
  26S proteasome subunit P45 family (TIGR01242; HMM-score: 12)
• TheSEED: data available for NCTC8325
• PFAM:
  P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 68.9)
  and 20 more
  Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 28.9)
  AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 24.8)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 24.7)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 21.3)
  DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 20)
  Zeta_toxin; Zeta toxin (PF06414; HMM-score: 19.2)
  AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 18.3)
  AAA_30; AAA domain (PF13604; HMM-score: 18.1)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 17.8)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.9)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.6)
  DUF2791; P-loop Domain of unknown function (DUF2791) (PF10923; HMM-score: 16.3)
  PIF1; PIF1-like helicase (PF05970; HMM-score: 16.1)
  Torsin; Torsin (PF06309; HMM-score: 14.1)
  Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 13.1)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 13.1)
  NACHT; NACHT domain (PF05729; HMM-score: 12.9)
  CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 12.7)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 12.6)
  ABC_tran; ABC transporter (PF00005; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.130401
  • TAT(Tat/SPI): 0.002622
  • LIPO(Sec/SPII): 0.021578
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446725749 NCBI
• RefSeq: WP_000803062 NCBI
• UniProt: see NWMN_1004

⊟Protein sequence

• MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKKHPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVKNYKPQNEKQVHAKQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDRIKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications