From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
  • pan locus tag?: SAUPAN001648000
  • symbol: NWMN_RS05650
  • pan gene symbol?: —
  • synonym:
  • product: DNA replication protein DnaC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
  • symbol: NWMN_RS05650
  • product: DNA replication protein DnaC
  • replicon: chromosome
  • strand: +
  • coordinates: 1109400..1110179
  • length: 780
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1109400..1110179) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1004

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAACCACTATTCAGCGAAAAGATAAACGAAAGCTTGAAAAAATATCAACCTACTCAT
    GTCGAAAAAGGATTGAAATGTGAGAGATGTGGAAGTGAATACGACTTATATAAGTTTGCT
    CCTACTAAAAAACACCCGAATGGTTACGAGTATAAAGACGGTTGCAAATGTGAAATCTAT
    GAGGAATATAAGCGAAACAAGCAACGGAAGATAAACAACATATTCAATCAATCAAACGTT
    AATCCGTCTTTAAGAGATGCAACAGTCAAAAACTACAAGCCACAAAATGAAAAACAAGTA
    CACGCTAAACAAACAGCAATAGAGTACGTACAAGGCTTCTCTACAAAAGAACCAAAATCA
    TTAATATTGCAAGGTTCATACGGAACTGGTAAAAGCCACCTAGCATACGCTATCGCAAAA
    GCAGTCAAAGCTAAAGGGCATACGGTTGCTTTTATGCACATACCAATGTTGATGGATCGT
    ATCAAAGCGACATACAACAAAAATGCAGTAGAGACTACAGACGAGCTAGTCAGATTGCTA
    AGTGATATTGATTTACTTGTACTAGATGATATGGGTGTAGAAAACACAGAGCACACTTTA
    AATAAACTTTTCAGCATTGTTGATAACAGAGTAGGTAAAAACAACATCTTTACAACTAAC
    TTTAGTGATAAAGAACTAAATCAAAATATGAACTGGCAACGTATCAATTCAAGAATGAAA
    CACAATGCGAGAAAAGTAAGAGTAATCGGAGACGATTTCAGGGAGCGAGATGCATGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS05650 [old locus tag: NWMN_1004 ]
  • symbol: NWMN_RS05650
  • description: DNA replication protein DnaC
  • length: 259
  • theoretical pI: 9.83133
  • theoretical MW: 30058.1
  • GRAVY: -0.847876

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 41.5)
    and 7 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 30.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 14)
    Genetic information processing Protein synthesis tRNA and rRNA base modification sulfur relay protein TusD/DsrE (TIGR03012; HMM-score: 13.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 13.5)
    Cellular processes Cellular processes Cell division ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 13)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 13)
    26S proteasome subunit P45 family (TIGR01242; HMM-score: 12)
  • TheSEED: see NWMN_1004
  • PFAM:
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 66.6)
    and 29 more
    Bac_DnaA; Bacterial DnaA ATPAse domain (PF00308; HMM-score: 30)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 26.3)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 24.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 21.1)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 20.9)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 19.4)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 19)
    AAA_30; AAA domain (PF13604; HMM-score: 18.5)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 18.1)
    AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 17.5)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 17.3)
    BrxC_BrxD; BREX system ATP-binding protein BrxC/D (PF10923; HMM-score: 16.6)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 15.9)
    CheY (CL0304) Response_reg_2; Response receiver domain (PF19192; HMM-score: 15.8)
    S5 (CL0329) Morc6_S5; Morc6 ribosomal protein S5 domain 2-like (PF17942; HMM-score: 15.7)
    P-loop_NTPase (CL0023) Zeta_toxin; Zeta toxin (PF06414; HMM-score: 15.2)
    no clan defined DUF6371; Domain of unknown function (DUF6371) (PF19898; HMM-score: 15.2)
    RING (CL0229) STL11_N; STL11, N-terminal (PF21369; HMM-score: 14.7)
    P-loop_NTPase (CL0023) Torsin; Torsin (PF06309; HMM-score: 14.1)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14)
    AAA_14; AAA domain (PF13173; HMM-score: 13.9)
    Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 13.1)
    NACHT; NACHT domain (PF05729; HMM-score: 13)
    AB_hydrolase (CL0028) Mbeg1-like; Mbeg1-like (PF11187; HMM-score: 12.7)
    NADP_Rossmann (CL0063) Eno-Rase_NADH_b; NAD(P)H binding domain of trans-2-enoyl-CoA reductase (PF12242; HMM-score: 11.1)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 10.7)
    AAA_11; AAA domain (PF13086; HMM-score: 10)
    ABC_tran; ABC transporter (PF00005; HMM-score: 9.9)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9816
    • Cytoplasmic Membrane Score: 0.0034
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0149
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.130401
    • TAT(Tat/SPI): 0.002622
    • LIPO(Sec/SPII): 0.021578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKKHPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVKNYKPQNEKQVHAKQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDRIKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS05650&oldid=82576"