PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
• pan locus tag^?: SAUPAN003767000
• symbol: NWMN_RS07235
• pan gene symbol^?: trpB
• synonym:
• product: tryptophan synthase subunit beta

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
• symbol: NWMN_RS07235
• product: tryptophan synthase subunit beta
• replicon: chromosome
• strand: +
• coordinates: 1414774..1415988
• length: 1215
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1414774..1415988) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1284

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAATAAACAAATACAAACAGAAGCAGATGAATTAGGTTTCTTTGGTGAATATGGAGGG
  CAATATGTTCCAGAAACATTAATGCCAGCAATTATTGAGTTGAAAAAAGCTTATAAAGAG
  GCAAAAGCAGACCCAGAGTTTCAAAGAGAACTGGAATACTATTTATCAGAGTATGTAGGA
  CGCGCGACACCACTTACATATGCTGCATCATATACTGAAAGCCTAGGTGGCGCTAAAATA
  TATTTGAAACGAGAGGATCTAAATCATACAGGCGCCCATAAAATTAATAATGCGTTAGGT
  CAAGCGTTGCTTGCTAAAAGAATGGGCAAGAAGAAGCTTGTTGCTGAAACTGGTGCGGGT
  CAACATGGTGTAGCTAGTGCTACGGTTGCTGCATTATTTGATATGGAACTTGTTGTCTTT
  ATGGGAAGTGAAGATATTAAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGT
  GCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCA
  TTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT
  CCAGACCCGTTCCCAACGATTGTTAGAGATTTTCAGAGTGTGATTGGTAAAGAAATAAAA
  TCACAGATATTGAAGAAAGAAGGTCGACTTCCGGATGCAATTGTAGCATGTATCGGTGGT
  GGCTCAAATGCAATCGGTACATTTTATCCATTTATTAAAGATGATGTTGCATTATACGGT
  GTTGAAGCCGCAGGTCAAGGCGATGATACTGATAAACATGCACTTGCAATTGGCAAAGGA
  TCACCTGGCGTATTACATGGTACTAAAATGTATTTAATTCAAGATGAAGATGGGCAAGTG
  CAACTAGCACATTCTATTTCAGCAGGACTTGATTATCCTGGTATTGGACCAGAACATTCT
  TATTACCACGACATTGGTAGAGTAACTTTTGAAAATGCTAGTGATACACAAGCAATGAAT
  GCTTTAATCAACTTTACAAAACATGAAGGTATTATACCTGCAATTGAAAGTGCACATGCA
  CTGAGTTATGTTGAAAGACTAGCGCCTACGATGTCGAAAGAAGATATTATTGTAGTAACT
  ATTTCTGGACGTGGCGATAAAGATATGGAAACAATTAGACAATATATGGTAGAGCGAGGT
  CTTGCAAATGACTAA
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  1215
  

⊟Protein

⊟General

• locus tag: NWMN_RS07235 [old locus tag: NWMN_1284 ]
• symbol: NWMN_RS07235
• description: tryptophan synthase subunit beta
• length: 404
• theoretical pI: 5.07529
• theoretical MW: 43964.5
• GRAVY: -0.223762

⊟Function

• reaction:
  EC 4.2.1.20?  ExPASy
  Tryptophan synthase L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H₂O
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 650.9)
  and 11 more
  Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 181.4)
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 44.3)
  Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 32.1)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 30.6)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 30)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 28.5)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 28)
  ectoine utilization protein EutB (TIGR02991; HMM-score: 26.7)
  Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 24.4)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 16)
  pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 14.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 134.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00892
  • TAT(Tat/SPI): 0.000876
  • LIPO(Sec/SPII): 0.001005
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446964081 NCBI
• RefSeq: WP_001041337 NCBI
• UniProt: see NWMN_1284

⊟Protein sequence

• MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKADPEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLGAKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKEIKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALAIGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENASDTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETIRQYMVERGLAND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: T-box(Trp), CodY see NWMN_1284

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications