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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07455 [old locus tag: NWMN_1325 ]
  • pan locus tag?: SAUPAN003830000
  • symbol: NWMN_RS07455
  • pan gene symbol?: sucB
  • synonym:
  • product: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07455 [old locus tag: NWMN_1325 ]
  • symbol: NWMN_RS07455
  • product: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
  • replicon: chromosome
  • strand: -
  • coordinates: 1455873..1457141
  • length: 1269
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1455873..1457141) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1325

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCAGAGGTTAAAGTTCCAGAATTAGCAGAATCTATTACAGAAGGTACCATTGCAGAA
    TGGTTGAAAAACGTAGGGGATAGCGTAGAAAAAGGTGAAGCTATTCTTGAATTAGAAACT
    GATAAAGTTAATGTCGAAGTTGTATCTGAAGAAGCAGGTGTATTATCTGAACAACTTGCA
    AGTGAAGGCGACACTGTAGAAGTTGGACAAGCAATTGCTATCATCGGCGAAGGTAGTGGC
    AATGCTTCTAAAGAAAATAGTAACGACAATACTCCACAACAAAATGAAGAAACAAATAAT
    AAAAAAGAAGAAACAACAAATAATTCGGTAGATAAAGCTGAAGTAAATCAAGCAAATGAT
    GACAATCAGCAACGTATTAATGCTACGCCTTCTGCGCGTCGATATGCTCGTGAAAATGGT
    GTGAATCTTGCTGAAGTAAGTCCGAAAACAAATGATGTGGTTCGTAAAGAAGATATTGAT
    AAGAAACAACAGGCACCGGCATCAACACAAACAACACAACAAGCATCTGCAAAAGAAGAG
    AAAAAATACAATCAATATCCTACAAAACCAGTGATTCGTGAAAAAATGTCACGTAGAAAG
    AAAACAGCTGCCAAAAAATTATTAGAGGTATCTAATAATACAGCTATGTTAACAACATTT
    AACGAAGTTGACATGACAAATGTTATGGAATTGCGTAAACGTAAGAAAGAACAATTTATG
    AAAGATCATGATGGTACTAAATTAGGATTTATGTCATTCTTTACTAAAGCTTCTGTAGCA
    GCTTTGAAAAAGTATCCAGAAGTTAATGCAGAAATCGACGGCGACGACATGATTACGAAA
    CAATATTATGATATTGGTGTAGCTGTTTCTACAGATGATGGATTATTAGTACCATTTGTA
    AGAGATTGTGATAAAAAGAATTTTGCAGAAATCGAAGCAGAAATTGCTAATTTAGCAGTT
    AAAGCACGAGAGAAAAAACTTGGCTTAGATGATATGGTTAATGGTTCATTTACGATTACA
    AATGGCGGTATTTTTGGATCAATGATGAGTACGCCAATTATCAATGGTAATCAAGCTGCA
    ATCTTAGGCATGCATTCAATTATTACAAGACCAATTGCGATTGATCAAGATACAATCGAA
    AATCGTCCAATGATGTATATTGCATTAAGCTATGATCATAGAATTATTGACGGTAAAGAA
    GCAGTTGGATTCTTAAAAACAATTAAAGAATTAATTGAAAACCCAGAAGACTTATTATTA
    GAATCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07455 [old locus tag: NWMN_1325 ]
  • symbol: NWMN_RS07455
  • description: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
  • length: 422
  • theoretical pI: 4.57447
  • theoretical MW: 46673.1
  • GRAVY: -0.611848

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.61?  ExPASy
    Dihydrolipoyllysine-residue succinyltransferase Succinyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-succinyldihydrolipoyl)lysine
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 610.9)
    and 12 more
    2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 303.8)
    Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 269.1)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (TIGR01349; EC 2.3.1.12; HMM-score: 249.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 22.6)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 22.6)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 21.4)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 15.1)
    amphi-Trp domain (TIGR04354; HMM-score: 14.4)
    glycine cleavage protein H-like protein (TIGR03077; HMM-score: 10)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism thymidylate synthase, flavin-dependent (TIGR02170; EC 2.1.1.148; HMM-score: 7.3)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 5.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, Trk family (TIGR00934; HMM-score: 3.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    CoA-acyltrans (CL0149) 2-oxoacid_dh; 2-oxoacid dehydrogenases acyltransferase (catalytic domain) (PF00198; HMM-score: 266.8)
    and 9 more
    Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 76.6)
    no clan defined E3_binding; e3 binding domain (PF02817; HMM-score: 29.1)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 21.9)
    HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 15.9)
    RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 15.5)
    no clan defined IF3_N; Translation initiation factor IF-3, N-terminal domain (PF05198; HMM-score: 14.5)
    Hybrid (CL0105) PYNP_C; Pyrimidine nucleoside phosphorylase C-terminal domain (PF07831; HMM-score: 13.6)
    P-loop_NTPase (CL0023) LAP1C; Lamina-associated polypeptide 1C (LAP1C) (PF05609; HMM-score: 6.6)
    no clan defined DUF966; Domain of unknown function (DUF966) (PF06136; HMM-score: 6.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: (R)-lipoate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003516
    • TAT(Tat/SPI): 0.000409
    • LIPO(Sec/SPII): 0.000396
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLLES

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS05395dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    NWMN_RS074602-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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