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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07670 [old locus tag: NWMN_1361 ]
  • pan locus tag?: SAUPAN003910000
  • symbol: NWMN_RS07670
  • pan gene symbol?: pbp2
  • synonym:
  • product: penicillin-binding protein 2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07670 [old locus tag: NWMN_1361 ]
  • symbol: NWMN_RS07670
  • product: penicillin-binding protein 2
  • replicon: chromosome
  • strand: +
  • coordinates: 1524397..1526580
  • length: 2184
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1524397..1526580) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1361

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACGGAAAACAAAGGATCTTCTCAGCCTAAGAAAAACGGTAATAATGGTGGGAAATCC
    AACTCAAAAAAGAATAGAAATGTGAAGAGAACGATTATTAAGATTATTGGCTTCATGATT
    ATTGCATTTTTCGTTGTTCTTTTACTAGGTATCTTATTGTTTGCTTATTATGCTTGGAAA
    GCACCTGCTTTTACCGAAGCTAAATTACAAGATCCGATTCCTGCAAAGATATATGACAAG
    AACGGAGAACTTGTTAAAACATTAGATAATGGCCAAAGACATGAGCATGTAAATTTAAAA
    GACGTGCCGAAATCAATGAAAGACGCAGTACTTGCAACTGAAGACAATCGTTTCTACGAA
    CATGGCGCACTTGATTATAAACGTTTATTCGGTGCAATTGGTAAGAACTTGACTGGTGGA
    TTTGGTTCTGAAGGTGCCTCAACATTAACACAACAAGTTGTTAAAGATGCATTTTTATCA
    CAACATAAATCTATTGGACGTAAAGCTCAAGAAGCATACTTATCATATCGTTTAGAACAA
    GAGTATAGTAAAGATGATATCTTCCAAGTATATCTAAACAAAATTTACTATTCTGATGGC
    GTAACAGGTATTAAAGCTGCTGCTAAGTATTACTTTAATAAAGATTTAAAAGATTTAAAC
    TTAGCGGAAGAAGCTTATTTAGCCGGTTTACCTCAGGTTCCAAACAACTATAATATTTAT
    GATCATCCAAAAGCTGCTGAAGATCGTAAAAACACTGTTTTATACTTAATGCATTATCAT
    AAACGCATTACAGATAAACAGTGGGAAGATGCTAAGAAAATCGATTTAAAAGCGAACTTA
    GTAAATCGTACTCCTGAAGAACGTCAAAACATTGATACAAATCAAGATTCTGAGTATAAT
    TCATACGTTAACTTTGTAAAATCTGAATTAATGAATAATAAAGCATTCAAAGATGAAAAT
    TTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAA
    AAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTT
    GGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGAT
    TTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTA
    AAACCTTTCTTAGCGTATGGACCTGCCATTGAAAATATGAAATGGGCAACAAACCATGCG
    ATTCAAGATGAATCTTCATATCAAGTTGATGGTTCTACATTTAGAAACTATGATACGAAG
    AGTCACGGTACTGTATCTATTTATGATGCTTTACGACAAAGTTTCAATATCCCAGCTTTA
    AAAGCTTGGCAATCAGTTAAGCAAAATGCTGGTAATGATGCACCTAAGAAATTCGCTGCC
    AAACTTGGCTTAAACTACGAAGGCGATATTGGTCCATCTGAAGTACTTGGTGGTTCTGCT
    TCAGAATTCTCACCAACACAATTAGCATCAGCATTTGCTGCAATCGCTAACGGTGGTACT
    TATAACAACGCGCATTCAATTCAAAAAGTAGTTACTCGTGATGGTGAAACAATCGAATAC
    GATCATACTAGCCATAAAGCGATGAGTGATTACACTGCATACATGTTAGCTGAGATGCTA
    AAAGGTACATTTAAACCATATGGTTCTGCATATGGCCATGGTGTATCTGGAGTAAATATG
    GGTGCTAAGACAGGTACTGGTACTTACGGTGCTGAAACTTATTCACAATATAATTTACCT
    GATAATGCAGCGAAAGACGTGTGGATTAACGGCTTTACACCTCAATACACTATGTCAGTG
    TGGATGGGCTTCAGTAAAGTTAAACAATATGGTGAAAACTCATTTGTGGGACATAGCCAA
    CAAGAATATCCACAGTTCTTATATGAAAATGTGATGTCAAAAATTTCATCTAGAGATGGC
    GAAGACTTTAAACGTCCTAGCTCAGTAAGTGGTAGTATCCCATCAATCAATGTTTCTGGT
    AGTCAAGATAACAACACTACAAATCGTAGTACACACGGTGGTAGTGACACATCAGCAAAC
    AGCAGTGGTACTGCACAATCAAATAACAATACTAGATCTCAACAATCTAGAAACAGCGGT
    GGATTAACAGGTATATTCAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2184

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07670 [old locus tag: NWMN_1361 ]
  • symbol: NWMN_RS07670
  • description: penicillin-binding protein 2
  • length: 727
  • theoretical pI: 9.13755
  • theoretical MW: 80425.8
  • GRAVY: -0.681843

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 452)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 243.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 234.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 124.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 68)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 26)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 26)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 201.3)
    and 4 more
    Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 98.3)
    no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.1)
    DUF3671; Protein of unknown function (PF12420; HMM-score: 13.7)
    Pox_EPC_I2-L1; Poxvirus entry protein complex L1 and I2 (PF12575; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001521
    • TAT(Tat/SPI): 0.000338
    • LIPO(Sec/SPII): 0.022601
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    NWMN_RS04080preprotein translocase subunit SecA  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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