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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1490 [new locus tag: SACOL_RS07590 ]
- pan locus tag?: SAUPAN003910000
- symbol: pbp2
- pan gene symbol?: pbp2
- synonym:
- product: penicillin-binding protein 2
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1490 [new locus tag: SACOL_RS07590 ]
- symbol: pbp2
- product: penicillin-binding protein 2
- replicon: chromosome
- strand: +
- coordinates: 1528217..1530400
- length: 2184
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237601 NCBI
- RefSeq: YP_186334 NCBI
- BioCyc: see SACOL_RS07590
- MicrobesOnline: 912942 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2161ATGACGGAAAACAAAGGATCTTCTCAGCCTAAGAAAAACGGTAATAATGGTGGGAAATCC
AACTCAAAAAAGAATAGAAATGTGAAGAGAACGATTATTAAGATTATTGGCTTCATGATT
ATTGCATTTTTCGTTGTTCTTTTACTAGGTATCTTATTGTTTGCTTATTATGCTTGGAAA
GCACCTGCTTTTACCGAAGCTAAATTACAAGATCCGATTCCTGCAAAGATATATGACAAG
AACGGAGAACTTGTTAAAACATTAGATAATGGCCAAAGACATGAGCATGTAAATTTAAAA
GACGTGCCGAAATCAATGAAAGACGCAGTACTTGCAACTGAAGACAATCGTTTCTACGAA
CATGGCGCACTTGATTATAAACGTTTATTCGGTGCAATTGGTAAGAACTTGACTGGTGGA
TTTGGTTCTGAAGGTGCCTCAACATTAACACAACAAGTTGTTAAAGATGCATTTTTATCA
CAACATAAATCTATTGGACGTAAAGCTCAAGAAGCATACTTATCATATCGTTTAGAACAA
GAGTATAGTAAAGATGATATCTTCCAAGTATATCTAAACAAAATTTACTATTCTGATGGC
GTAACAGGTATTAAAGCTGCTGCTAAGTATTACTTTAATAAAGATTTAAAAGATTTAAAC
TTAGCGGAAGAAGCTTATTTAGCCGGTTTACCTCAGGTTCCAAACAACTATAATATTTAT
GATCATCCAAAAGCTGCTGAAGATCGTAAAAACACTGTTTTATACTTAATGCATTATCAT
AAACGCATTACAGATAAACAGTGGGAAGATGCTAAGAAAATCGATTTAAAAGCGAACTTA
GTAAATCGTACTCCTGAAGAACGTCAAAACATTGATACAAATCAAGATTCTGAGTATAAT
TCATACGTTAACTTTGTAAAATCTGAATTAATGAATAATAAAGCATTCAAAGATGAAAAT
TTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAA
AAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTT
GGTGCAACAATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGAT
TTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTA
AAACCTTTCTTAGCGTATGGACCTGCCATTGAAAATATGAAATGGGCAACAAACCATGCG
ATTCAAGATGAATCTTCATATCAAGTTGATGGTTCTACATTTAGAAACTATGATACGAAG
AGTCACGGTACTGTATCTATTTATGATGCTTTACGACAAAGTTTCAATATCCCAGCTTTA
AAAGCTTGGCAATCAGTTAAGCAAAATGCTGGTAATGATGCACCTAAGAAATTCGCTGCC
AAGCTTGGCTTAAACTACGAAGGCGATATTGGTCCATCTGAAGTACTTGGTGGTTCTGCT
TCAGAATTCTCACCAACACAATTAGCATCAGCATTTGCTGCAATCGCTAACGGTGGTACT
TATAACAACGCGCATTCAATTCAAAAAGTAGTTACTCGTGATGGTGAAACAATCGAATAC
GATCATACTAGCCATAAAGCGATGAGTGATTACACTGCATACATGTTAGCTGAGATGCTA
AAAGGTACATTTAAACCATATGGTTCTGCATATGGCCATGGTGTATCTGGAGTAAATATG
GGTGCTAAGACAGGTACTGGTACTTACGGTGCTGAAACTTATTCACAATATAATTTACCT
GATAATGCAGCGAAAGACGTGTGGATTAACGGCTTTACACCTCAATACACTATGTCAGTG
TGGATGGGCTTCAGTAAAGTTAAACAATATGGTGAAAACTCATTTGTGGGACATAGCCAA
CAAGAATATCCACAGTTCTTATATGAAAATGTGATGTCAAAAATTTCATCTAGAGATGGC
GAAGACTTTAAACGTCCTAGCTCAGTAAGTGGTAGTATCCCATCAATCAATGTTTCTGGT
AGTCAAGATAACAACACTACAAATCGTAGTACACACGGTGGTAGTGACACATCAGCAAAC
AGCAGTGGTACTGCACAATCAAATAACAATACTAGATCTCAACAATCTAGAAACAGCGGT
GGATTAACAGGTATATTCAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1490 [new locus tag: SACOL_RS07590 ]
- symbol: Pbp2
- description: penicillin-binding protein 2
- length: 727
- theoretical pI: 9.13755
- theoretical MW: 80425.8
- GRAVY: -0.681843
⊟Function[edit | edit source]
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 452)and 6 moreCell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 243.9)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 234.5)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 124.5)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 68)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 26)Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 26)
- TheSEED :
- Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)
- Penicillin-binding protein 1A/1B (PBP1)
Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Control of cell elongation - division cycle in Bacilli Penicillin-binding protein 1A/1B (PBP1)and 1 more - PFAM: Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 201.3)and 4 moreBeta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 98.3)no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.1)DUF3671; Protein of unknown function (PF12420; HMM-score: 13.7)Pox_EPC_I2-L1; Poxvirus entry protein complex L1 and I2 (PF12575; HMM-score: 11.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.17
- Cytoplasmic Membrane Score: 9.51
- Cellwall Score: 0.16
- Extracellular Score: 0.15
- Internal Helix: 1
- LocateP: N-terminally anchored (No CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 5
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001521
- TAT(Tat/SPI): 0.000338
- LIPO(Sec/SPII): 0.022601
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Signal peptide containing [1] [2] [3] [4]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SACOL1488 > recU > pbp2
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ 5.0 5.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Andrew L Lovering, Liza De Castro, Natalie C J Strynadka
Identification of dynamic structural motifs involved in peptidoglycan glycosyltransfer.
J Mol Biol: 2008, 383(1);167-77
[PubMed:18760285] [WorldCat.org] [DOI] (I p)