From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08165 [old locus tag: NWMN_1447 ]
  • pan locus tag?: SAUPAN004122000
  • symbol: NWMN_RS08165
  • pan gene symbol?: comGB
  • synonym:
  • product: competence protein ComGB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08165 [old locus tag: NWMN_1447 ]
  • symbol: NWMN_RS08165
  • product: competence protein ComGB
  • replicon: chromosome
  • strand: -
  • coordinates: 1615073..1616143
  • length: 1071
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1615073..1616143) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1447

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAG
    GCCCAACAAATCGACTTACTTTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTG
    TATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATAAAAATAAGCAATTAGGTACC
    ACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
    TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGAC
    GTTCTAGAAGAAACCGTAAATTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTA
    AAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTATTGCTATGATTATTATATTA
    AACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
    TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATG
    CTCATAATAGTATCTATGTTGGCTATTATTATGAAATTAATTTATAACAATTTAAATATG
    CTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATATCAGGCTATTTCCAATTATTT
    AAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
    TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAA
    TACTTATTAACTTATTCAGAAATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAA
    TGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTGAAAAGAGAGGGAAGCTAGAA
    GTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
    CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATT
    TATTTAGTAATTATGTTACCAATGTTTCAAATGATGCAAAGTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS08165 [old locus tag: NWMN_1447 ]
  • symbol: NWMN_RS08165
  • description: competence protein ComGB
  • length: 356
  • theoretical pI: 10.0111
  • theoretical MW: 41364.5
  • GRAVY: 0.410674

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein F (TIGR02120; HMM-score: 95.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 81.2)
    and 2 more
    E1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 9.3)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 6.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005297
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.001434
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLSSFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]