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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08645 [old locus tag: NWMN_1541 ]
  • pan locus tag?: SAUPAN004245000
  • symbol: NWMN_RS08645
  • pan gene symbol?: tgt
  • synonym:
  • product: tRNA guanosine(34) transglycosylase Tgt

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08645 [old locus tag: NWMN_1541 ]
  • symbol: NWMN_RS08645
  • product: tRNA guanosine(34) transglycosylase Tgt
  • replicon: chromosome
  • strand: -
  • coordinates: 1709414..1710553
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1709414..1710553) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1541

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAGGT
    ATCGTGCACACACCACACGGTTCATTTGAAACACCTATGTTTATGCCAGTTGGTACTAAA
    GCAACCGTTAAAACAATGAGTCCAGAAGAGTTAAGACAAATTGAAGCAAAAATCATTTTG
    GGCAACACATATCATTTGTGGTTACAACCCGGAAATGATATTATCAAACACGCTGGGGGA
    TTACATAAATTCATGAATTGGGATGGTCCGATTCTTACAGATTCAGGCGGTTTCCAAGTG
    TTTAGTTTAAGTAATTTACGTAAAATTACAGAAGAAGGCGTGGAATTTAGACATCATACT
    AATGGGTCTAAATTATTTTTGAGTCCTGAGAAATCAATGCAAATTCAAAATGATTTAGGA
    TCTGATATTATGATGGCATTTGATGAATGTCCACCGATGCCTGCTGAATATGATTATGTA
    AAAAAATCTATTGAACGTACAACACGTTGGGCGAAAAGATGTCTAGATGCACACCAAAGA
    CCTGAAGATCAAGCATTGTTCGGCATTATACAAGGTGGCGAATATGAAGATTTAAGAGAA
    CAAAGTGCAAAGGATTTAGTAGAATTAGATTTTCCTGGTTATGCAATCGGCGGTTTGTCA
    GTTGGTGAACCTAAACCGGTTATGTATAAAATGGTTGAACATACAGAGCAGTTTATGCCT
    AAAGATAAACCAAGATATTTAATGGGTGTAGGATCTCCAGATGCGTTAATCGAATGTAGT
    ATTCGCGGCATGGATATGTTTGATTGTGTCTTACCGACACGTATTGCCAGAAATGGTACT
    TGTATGACATCGCAAGGTCGTTTAGTTATTAAAAATGCAAAATTTGCAGATGATTTAAGA
    CCGTTAGATGAGAATTGTGACTGTTATACATGTCAAAACTATTCAAGAGCGTATATACGT
    CATTTAATCAAGGCAGAGGAAACTTTTGGTATTCGTCTTACTACTATTCATAATTTACAT
    TTTCTGCTAAAATTAATGGAAGATATAAGACAAGCCATTCGAGAAGATCGTCTTTTAGAT
    TTCAAAGAAGAATTCTTCGAGCAATATGGATTAAATGTTGAGAACCCAAAAAACTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS08645 [old locus tag: NWMN_1541 ]
  • symbol: NWMN_RS08645
  • description: tRNA guanosine(34) transglycosylase Tgt
  • length: 379
  • theoretical pI: 6.3237
  • theoretical MW: 43309.5
  • GRAVY: -0.435356

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.29?  ExPASy
    tRNA-guanine34 transglycosylase Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine transglycosylase (TIGR00430; EC 2.4.2.29; HMM-score: 607.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine family transglycosylase (TIGR00449; EC 2.4.2.-; HMM-score: 575.3)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-guanine(15) transglycosylase (TIGR00432; EC 2.4.2.48; HMM-score: 121.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined TGT; Queuine tRNA-ribosyltransferase (PF01702; HMM-score: 319.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008848
    • TAT(Tat/SPI): 0.001214
    • LIPO(Sec/SPII): 0.001649
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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