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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS09330 [old locus tag: NWMN_1662 ]
- pan locus tag?: SAUPAN004458000
- symbol: NWMN_RS09330
- pan gene symbol?: ribD
- synonym:
- product: riboflavin biosynthesis protein RibD
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS09330 [old locus tag: NWMN_1662 ]
- symbol: NWMN_RS09330
- product: riboflavin biosynthesis protein RibD
- replicon: chromosome
- strand: -
- coordinates: 1857758..1858789
- length: 1032
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGGATTATGCGATTCAACTTGCAAATATGGTACAAGGTCAAACAGGTGTTAATCCACCC
GTTGGCGCTGTTGTAGTTAAAGAAGGTAGGATTGTTGGTATTGGTGCACACTTGAGAAAA
GGTGACAAGCATGCGGAGGTTCAAGCACTTGATATGGCACAACAAAATGCTGAAGGTGCG
ACGATTTATATTACGTTAGAGCCATGTAGTCATTTTGGTTCAACACCACCCTGTGTTAAC
AAAATAATTGATTGTAAGATAGCAAAAGTAGTATACGCAACAAAAGACAATTCGTTAGAC
ACACATGGTGATGAGACGTTACGGGCTCACGGTATTGAGGTTGAATGCGTTGATGATGAA
CGGGCATCACAATTATACCAAGACTTTTTTAAAGCAAAAGCAAAGCAACTGCCACAAATT
ACAGTGAAAGTATCTGCAAGTTTAGATGGTAAACAAGCGAATGATAATGGACAAAGTCAA
TGGATTACTAACAAAGAGGTTAAACAAGATGTCTATAAGTTAAGACATCGACACGACGCA
GTGTTAACTGGAAGACGTACAGTTGAATTAGATGATCCACAATATACTACACGTATTCAA
GATGGAAAAAACCCTATAAAAGTAATATTGTCTAAGTCTGGGAATATTCATTTTAATCAG
CAAATTTATCAAGATGAATCAACACCAATTTGGATATATACTGAAAATCCAAATTTAACA
ACCAATCAAACACATATTGAAATTATTTACTTGAAGTCTTGCGATTTAACAACAATTCTT
CACAATTTATATAAAAGAGGAGTTGGAACTTTGCTAGTCGAGGCAGGTCCAACCACTACT
TCAGAATTTCTCCAATCTAATTATATAGATGAATTTATTCTCTATTATGCCCCGAAATTA
ATTGGCGGATCTGGAAATTATCAATTTTATCAAACAAATGATGTGATTGAGATACCAGAT
GCGAACCAATTTGAAATTGTTCATTCCGAGTTATTAAATCAAAATGTTAAATTAACTTTA
CGAAAGAAGTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS09330 [old locus tag: NWMN_1662 ]
- symbol: NWMN_RS09330
- description: riboflavin biosynthesis protein RibD
- length: 343
- theoretical pI: 6.43647
- theoretical MW: 38459.2
- GRAVY: -0.442857
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibD (TIGR00326; EC 1.1.1.193,3.5.4.26; HMM-score: 302.8)and 3 moreriboflavin-specific deaminase C-terminal domain (TIGR00227; EC 3.5.4.-; HMM-score: 150.6)diaminohydroxyphosphoribosylaminopyrimidine reductase (TIGR01508; EC 1.1.1.-; HMM-score: 96.6)ComE operon protein 2 (TIGR02571; HMM-score: 15.7)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: DHFred (CL0387) RibD_C; RibD C-terminal domain (PF01872; HMM-score: 141.7)and 3 moreCDA (CL0109) dCMP_cyt_deam_1; Cytidine and deoxycytidylate deaminase zinc-binding region (PF00383; HMM-score: 63.4)MafB19-deam; MafB19-like deaminase (PF14437; HMM-score: 44)APOBEC_N; APOBEC-like N-terminal domain (PF08210; HMM-score: 13.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.017053
- TAT(Tat/SPI): 0.0013
- LIPO(Sec/SPII): 0.001828
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRKGDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNSLDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDNGQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSGNIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLLVEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSELLNQNVKLTLRKK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: FMN-box see NWMN_1662
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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