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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09460 [old locus tag: NWMN_1686 ]
  • pan locus tag?: SAUPAN004511000
  • symbol: NWMN_RS09460
  • pan gene symbol?: menE
  • synonym:
  • product: 2-succinylbenzoate-CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09460 [old locus tag: NWMN_1686 ]
  • symbol: NWMN_RS09460
  • product: 2-succinylbenzoate-CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1881110..1882588
  • length: 1479
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1881110..1882588) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1686

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGACTTTTGGTTATATAAACAAGCACAACAAAATGGACATCATATTGCGATAACAGAC
    GGTCAAGAATCTTATACTTATCAAAATTTATACTGTGAAGCGAGTCTATTGGCTAAAAGA
    CTCAAGGCTTATCAACAATCTCGTGTTGGGCTATACATAGATAATTCGATTCAATCGATC
    ATTTTAATACATGCATGTTGGTTGGCAAATATTGAAATTGCGATGATTAATACAAGGTTG
    ACACCTAATGAGATGACGAATCAGATGAAGTCAATCGATGTACAATTGATTTTTTGTACC
    TTGCCACTGGAATTACGAGGGTTTCAAATTGTATCGCTGGATGATATTGAATTCGCTGGA
    AGGGATATTACAACGAACAGTTTGTTGGACAACACAATGGGTATCCAATATGAAACATCG
    AATGAAACTGTGGTGCCGAAAGAGTCGCCGTCCAACATATTAAATACATCATTTAATTTA
    GATGACATTGCATCGATTATGTTTACATCAGGGACAACGGGCCCTCAAAAAGCGGTGCCA
    CAAACGTTTCGTAATCATTATGCCAGTGCAATCGGATGTAAAGAGAGCTTGGGATTTGAT
    CGTGACACTAATTGGCTATCTGTCTTGCCGATTTATCATATTTCGGGTCTCAGTGTACTT
    TTAAGAGCTGTTATTGAAGGTTTTACTGTGCGCATTGTTGATAAATTCAATGCCGAACAA
    ATTTTAACGATGATTAAAAATGAACGAATCACGCACATTTCGCTTGTGCCACAAACTTTA
    AATTGGCTTATGCAACAAGGTTTACATGAACCTTATAATTTACAAAAAATATTACTCGGC
    GGTGCTAAATTATCTGCCACTATGATAGAGACGGCATTACAATATAACCTGCCAATTTAT
    AATTCATTTGGTATGACTGAGACATGTTCGCAATTTTTAACAGCAACACCGGAAATGTTG
    CATGCACGTCCTGACACTGTAGGGATGCCAAGTGCCAATGTTGACGTAAAAATTAAAAAT
    CCTAATAAAGAAGGTCATGGAGAATTAATGATTAAAGGCGCCAATGTGATGAATGTATAT
    TTGTATCCAACAGATTTAACGGGTACGTTTGAAAATGGTTATTTTAATACGGGTGACATT
    GCTGAAATAGATCATGAAGGTTATGTCATGATTTATGACCGACGTAAAGATTTAATTATT
    AGTGGCGGTGAAAATATTTATCCATATCAAATTGAAACGGTAGCGAAGCAATTTCCAGGT
    ATCAGTGACGCAGTATGTGTAGGGCATCCTGATGATACTTGGGGCCAAGTGCCTAAATTA
    TATTTTGTCAGTGAAAGTGACATTTCAAAGGCACAATTGATTGCATATTTATCACAACAT
    TTAGCGAAATATAAAGTACCGAAACACTTTGAAAAGGTTGATACTTTACCTTATACATCA
    ACAGGTAAATTACAAAGAAATAAGTTGTATAGAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS09460 [old locus tag: NWMN_1686 ]
  • symbol: NWMN_RS09460
  • description: 2-succinylbenzoate-CoA ligase
  • length: 492
  • theoretical pI: 5.90461
  • theoretical MW: 55414.9
  • GRAVY: -0.192276

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.26?  ExPASy
    o-succinylbenzoate--CoA ligase ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 663.8)
    and 11 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 148.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 147.2)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 142.1)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 139.3)
    amino acid adenylation domain (TIGR01733; HMM-score: 117.5)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 91.1)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 79.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 58.6)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 30.2)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 16.9)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 14.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 259.4)
    and 2 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 50.1)
    no clan defined DUF4176; Domain of unknown function (DUF4176) (PF13780; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027554
    • TAT(Tat/SPI): 0.000304
    • LIPO(Sec/SPII): 0.002664
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAYQQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLPLELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNLDDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLSVLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQKILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANVDVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIYDRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISKAQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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