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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10370 [old locus tag: NWMN_1809 ]
  • pan locus tag?: SAUPAN001729000
  • symbol: NWMN_RS10370
  • pan gene symbol?:
  • synonym:
  • product: chromosome replication initiation protein DnaA

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS10370 [old locus tag: NWMN_1809 ]
  • symbol: NWMN_RS10370
  • product: chromosome replication initiation protein DnaA
  • replicon: chromosome
  • strand: -
  • coordinates: 2015799..2016587
  • length: 789
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (2015799..2016587) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1809

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACTGAGGAAAAACAAGAACCACAAGAAAAAGTAAGCATACTCAAAAAACTAAAGATA
    AATAATATCGCTGAGAAAAATAAAAGGAAATTCTATAAATTTGCAGTATACGGAAAAATT
    GGCTCAGGAAAAACCACGTTTGCTACAAGAGATAAAGACGCTTTCGTCATTGACATTAAC
    GAAGGTGGAACAACGGTTACTGACGAAGGATCAGACGTAGAAATCGAGAACTATCAACAC
    TTTGTTTATGTTGTAAATTTTTTACCTCAAATTTTACAGGAGATGAGAGAAAACGGACAA
    GAAATCAATGTTGTAGTTATTGAAACTATTCAAAAACTTAGAGATATGACATTGAATGAT
    GTGATGAAAAATAAGTCTAAAAAACCAACGTTTAATGATTGGGGAGAAGTTGCTGAACGA
    ATTGTCAGTATGTACAGATTAATAGGAAAACTTCAAGAAGAATACAAATTCCACTTTGTT
    ATTACAGGTCATGAAGGTATCAACAAAGATAAAGATGATGAAGGTAGCACTATCAACCCT
    ACTATCACTATTGAAGCGCAAGAACAAATTAAAAAAGCTATTACTTCTCAAAGTGATGTG
    TTAGCTAGGGCAATGATTGAAGAATTTGATGATAACGGAGAAAAGAAAGCTAGATATATT
    CTAAACGCTGAACCTTCTAATACGTTTGAAACAAAGATTAGACATTCACCTTCAATAACA
    ATTAACAATAAGAAATTTGCAAATCCTAGCATTACGGACGTAGTAGAAGCAATTAGAAAT
    GGAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    789

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS10370 [old locus tag: NWMN_1809 ]
  • symbol: NWMN_RS10370
  • description: chromosome replication initiation protein DnaA
  • length: 262
  • theoretical pI: 5.81837
  • theoretical MW: 29956.7
  • GRAVY: -0.657252

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage nucleotide-binding protein (TIGR01618; HMM-score: 26.5)
  • TheSEED: data available for NCTC8325
  • PFAM:
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 111.6)
    and 5 more
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 19.6)
    AAA_22; AAA domain (PF13401; HMM-score: 14.6)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 13.5)
    no clan defined Gasdermin; Gasdermin family (PF04598; HMM-score: 11.8)
    P-loop_NTPase (CL0023) AAA_17; AAA domain (PF13207; HMM-score: 8.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01491
    • TAT(Tat/SPI): 0.008703
    • LIPO(Sec/SPII): 0.000958
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGKTTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEINVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFVITGHEGINKDKDDEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKARYILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]