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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10475 [old locus tag: NWMN_1824 ]
  • pan locus tag?: SAUPAN004901000
  • symbol: NWMN_RS10475
  • pan gene symbol?: vraT
  • synonym:
  • product: transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS10475 [old locus tag: NWMN_1824 ]
  • symbol: NWMN_RS10475
  • product: transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2030865..2031566
  • length: 702
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (2030865..2031566) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1824

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACACACAAATATATATCAACGCAAATGTTGATCATTTTTACTGCATTAATGATTATT
    GCCAATTTTTACTACATATTTTTTGAAAAAATTGGCTTTTTACTCGTTCTATTATTGGGA
    TGTGTATTAGTTTATGTAGGATATCTTTATTTTCATAAAATACGTGGCCTTTTGGCGTTT
    TGGATAGGCGCGCTATTAATTGCATTCACATTATTGTCTAATAAGTATACAATCATCATC
    TTGTTCGTCTTTTTATTATTACTTATTGTGCGTTATTTAATACACAAGTTTAAACCAAAA
    AAAGTAGTTGCGACGGATGAGGTTATGACTTCACCATCTTTTATTAAACAAAAGTGGTTT
    GGTGAGCAACGTACACCAGTTTATGTATATAAGTGGGAAGATGTACAAATTCAACATGGA
    ATTGGCGACCTACATATTGACTTAACAAAAGCTGCAAATATTAAGGAAAATAATACCATT
    GTTGTTAGACACATTTTAGGTAAAGTGCAGGTTATATTGCCGGTTAATTACAATATTAAT
    TTACATGTAGCTGCTTTTTATGGAAGTACTTACGTGAATGAAAAATCATATAAAGTTGAA
    AATAACAATATTCATATTGAAGAAATGATGAAACCGGATAACTATACAGTTAATATCTAC
    GTATCAACGTTTATCGGAGACGTAGAGGTGATTTATCGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    702

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS10475 [old locus tag: NWMN_1824 ]
  • symbol: NWMN_RS10475
  • description: transporter
  • length: 233
  • theoretical pI: 9.49778
  • theoretical MW: 27204.1
  • GRAVY: 0.479399

Function[edit | edit source]

  • TIGRFAM:
    flavivirus envelope glycoprotein E, stem/anchor domain (TIGR04240; HMM-score: 9.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined DUF2154; Cell wall-active antibiotics response 4TMS YvqF (PF09922; HMM-score: 109.1)
    and 2 more
    MerC; MerC mercury resistance protein (PF03203; HMM-score: 8.9)
    SdpI; SdpI/YhfL protein family (PF13630; HMM-score: 6.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0013
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.07494
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLVYVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKVVATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTIVVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVNIYVSTFIGDVEVIYR

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]