PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11265 [old locus tag: NWMN_1953 ]
• pan locus tag^?: SAUPAN005291000
• symbol: NWMN_RS11265
• pan gene symbol^?: rex
• synonym:
• product: transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11265 [old locus tag: NWMN_1953 ]
• symbol: NWMN_RS11265
• product: transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2161492..2162127
• length: 636
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2161492..2162127) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1953

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
  601
  
  ATGAGTGACCAAGTTAAAATTCCTCGAGCAACTTTAAAACGTTTGCCGTTATATTATAGA
  TTTGTCAGTTCATTAAAATCTAAAGGTATAGATCGTGTAAATTCAAAAGCGATTAGCGAT
  GCGTTACAAATTGACTCGGCAACAATTCGTCGTGACTTTTCATATTTTGGCGAATTAGGT
  AAAAAAGGGTACGGATATAATATAGATAGTTTATTGGATTTCTTTAAATCTGAACTAAGC
  GAGAGTGACATGATCAAAATCGCAATTGTCGGAGTTGGGAACCTAGGGAAAGCTTTGCTC
  ACATATAACTTTTCAATACATGACGATATGACGATTACAGAAGCGTTTGACGTAAAAGAA
  GATGTTATTGGCCAGAAAATAGGGAACGTTATTGTTAAAGATAACGATGAATTAATAACA
  ACATTGAAGAAGGAAGAAATAGATGTTGTGATTCTAACTACACCAGAAAGAGTTGCACAG
  AAAGTTGCAGATGAACTCGTCCAAGCTGGTGTGAAAGGTATTTTAAACTTCACTCCTGGT
  AGAATTAATACGCCTTCAGATGTGCAAGTACATCAAATTGACTTAGGTATAGAATTACAG
  TCATTATTATTCTTTATGAAAAATTACAGTGAATAA
  60
  120
  180
  240
  300
  360
  420
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  540
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  636
  

⊟Protein

⊟General

• locus tag: NWMN_RS11265 [old locus tag: NWMN_1953 ]
• symbol: NWMN_RS11265
• description: transcriptional regulator
• length: 211
• theoretical pI: 5.12384
• theoretical MW: 23598.9
• GRAVY: -0.0995261

⊟Function

• TIGRFAM:
  diaminopimelate dehydrogenase (TIGR01921; EC 1.4.1.16; HMM-score: 21.3)
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 19.5)
  Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate reductase (TIGR00036; EC 1.17.1.8; HMM-score: 17.4)
  Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 17.3)
  and 7 more
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 15.8)
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 14.8)
  acetaldehyde dehydrogenase (acetylating) (TIGR03215; EC 1.2.1.10; HMM-score: 14.2)
  acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 14.1)
  undecaprenyl-phosphate glucose phosphotransferase (TIGR03023; EC 2.7.8.-; HMM-score: 13.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjU (TIGR03368; HMM-score: 12.2)
  exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 12)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) CoA_binding; CoA binding domain (PF02629; HMM-score: 91.4)
  and 10 more
  HTH (CL0123) Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 68.1)
  NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 25.3)
  GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 19.2)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.1)
  HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 16.4)
  no clan defined Packaging_FI; DNA packaging protein FI (PF14000; HMM-score: 15.3)
  NADP_Rossmann (CL0063) CoA_binding_2; CoA binding domain (PF13380; HMM-score: 15.1)
  PreATP-grasp (CL0483) GARS_N; Phosphoribosylglycinamide synthetase, N domain (PF02844; HMM-score: 14.7)
  P-loop_NTPase (CL0023) NB-ARC; NB-ARC domain (PF00931; HMM-score: 13)
  no clan defined MTD; methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase (PF01993; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by Rex*, TF important in Energy metabolism: see NWMN_1953

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007572
  • TAT(Tat/SPI): 0.000828
  • LIPO(Sec/SPII): 0.001442
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447206356 NCBI
• RefSeq: WP_001283612 NCBI
• UniProt: see NWMN_1953

⊟Protein sequence

• MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQIDSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTYNFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQKVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications