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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • pan locus tag?: SAUPAN005341000
  • symbol: NWMN_RS11430
  • pan gene symbol?: alr
  • synonym:
  • product: alanine racemase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • symbol: NWMN_RS11430
  • product: alanine racemase
  • replicon: chromosome
  • strand: -
  • coordinates: 2193212..2194360
  • length: 1149
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2193212..2194360) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1975

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCGGATAAATATTATAGATCTGCGTATATGAATGTAGATTTAAACGCTGTTGCATCA
    AATTTCAAAGTATTCAGTACATTGCATCCAAATAAAACAGTGATGGCTGTCGTTAAAGCC
    AATGCCTATGGACTAGGTAGTGTTAAAGTAGCACGTCATTTAATGGAAAATGGCGCCACA
    TTTTTTGCTGTAGCAACGTTAGATGAAGCGATAGAACTTAGAATGCATGGGATTACTGCT
    AAAATTTTAGTCTTAGGTGTGTTACCAGCTAAAGATATTGATAAAGCGATACAACACCGA
    GTTGCCTTAACGGTTCCGTCTAAACAGTGGTTGAAAGAAGCAATTAAAAACATTTCTGGT
    GAGCAAGAGAAAAAGTTATGGTTGCACATTAAATTAGATACAGGAATGGGACGTTTAGGT
    ATTAAAGATACTAAAACGTATCAAGAAGTGATTGAAATCATTCAACAATATGAGCAACTT
    GTATTTGAAGGCGTGTTTACACACTTTGCCTGTGCTGACGAACCAGGAGATATGACAACT
    GAACAATATCAACGTTTTAAAGATATGGTCAATGAAGCAATTAAACCTGAATATATACAT
    TGTCAGAACTCAGCAGGCTCTCTATTAATGGATTGCCAATTCTGTAATGCAATAAGACCA
    GGAATTTCCCTTTATGGATATTATCCATCAGAGTATGTACAGCAAAAAGTTAAAGTACAC
    CTTAAACCAAGTGTGCAATTAATTGCTAATGTAGTTCAAACAAAGACGCTACAAGCGGGT
    GAGTCTGTAAGTTATGGTGCAACTTATACAGCTACTGACCCAACTACAATAGCATTGTTA
    CCTATTGGATATGCAGATGGCTATTTACGCATAATGCAAGGTAGCTTTGTAAATGTAAAT
    GGTCATCAATGCGAAGTTATTGGTCGCGTATGTATGGATCAGACAATTGTTAAAGTGCCA
    GATCAAGTTAAAGCTGGAGATTCGGTGATTTTAATAGATAATCATAGAGAAAGTCCACAG
    TCGGTAGAGGTGGTAGCTGAAAAGCAACATACTATTAATTATGAAGTGCTTTGTAACTTG
    TCGAGACGTTTGCCGCGAATCTATCATGATGGTGATCAACGTTTTGTAACAAATGAATTG
    TTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1149

⊟Protein[edit | edit source]

Protein Data Bank: 3OO2

⊟General[edit | edit source]

  • locus tag: NWMN_RS11430 [old locus tag: NWMN_1975 ]
  • symbol: NWMN_RS11430
  • description: alanine racemase
  • length: 382
  • theoretical pI: 7.33759
  • theoretical MW: 42823
  • GRAVY: -0.158377

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemase L-alanine = D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 373.1)
    and 1 more
    PRTRC system protein B (TIGR03737; HMM-score: 12.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 223.5)
    and 2 more
    no clan defined Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 143.5)
    Styelin; Styelin A-E (PF17562; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014203
    • TAT(Tat/SPI): 0.002283
    • LIPO(Sec/SPII): 0.000643
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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