PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS12790 [old locus tag: NWMN_2218 ]
• pan locus tag^?: SAUPAN005796000
• symbol: NWMN_RS12790
• pan gene symbol^?: —
• synonym:
• product: alanine glycine permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS12790 [old locus tag: NWMN_2218 ]
• symbol: NWMN_RS12790
• product: alanine glycine permease
• replicon: chromosome
• strand: -
• coordinates: 2439483..2440862
• length: 1380
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2439483..2440862) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2218

⊟Phenotype

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⊟DNA sequence

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  ATGGAGAACAATGAACTACAAAGGGGATTGAGTGCCCGTCAAATTCAAATGATTGCACTT
  GGTGGTACGATTGGCGTGGGGCTTTTCATGGGTGCGACAAGTACAATTAAATGGACAGGC
  CCATCAGTTATCCTTGCATATTTAATTGCGGGTATCTTTTTATTTTTAATCATGAGAGCA
  ATGGGGGAAATGATTTATTTAAACCCTACAACAGGATCATTTGCAACATTTGCAAGTGAT
  TATATACATCCTGCAGCAGGTTATATGACAGCATGGAGTAATATATTCCAATGGATTGTA
  GTTGGTATGAGTGAGGTCATCGCAGTAGGAGAATATATGAAGTTTTGGTTCCCGGAATTG
  CCAACTTGGATTCCTGGTGTTATTGCTATTTTATTATTAATGGCAGCGAATTTATTCTCG
  GTAAAAGCGTTTGGAGAATTTGAATTTTGGTTTGCTTTAATTAAAGTTGTAACAATTATT
  TTAATGATTATTGCTGGTTTTGGTCTTATTTTCTTTGGTTTTGGAAATGGTGGCCATGCG
  GTAGGTATTTCTAATCTATGGACAAATGGCGGATTTATGCCAAATGGTATTGTTGGTTTC
  TTCTTTGCATTATCAATTGTAATTGGTTCATACCAAGGTGTGGAACTGATAGGTATTACG
  GCAGGTGAAACTAAAGATCCTCAGAAAAATATCGTTAAAGCAGTGAATGGTGTTATCTGG
  AGAATTTTAATTTTCTATTTAGGCGCTATTTTTGTTATTGTTTCAGTGTATCCTTGGAAT
  CAATTAGGAGACATTGGAAGTCCGTTTGTTGCAACATTTGCTAAAATCGGTATTACATTT
  GCAGCTGGATTAATTAACTTTGTTGTATTAACTGCAGCAATGTCAGGATGTAACTCAGGT
  ATTTTCAGTGCGAGTCGTATGATTTATACACTTGCTCATAAAGGTGAAATGCCGAAAATA
  TTTACTAAAATCATGAGAAATGGTGTACCATTGTACACTGTTGTAGCAGTATCTCTTGGT
  ATTTTAATTGGTGCTTTATTAAACGTAATTTTACCTTTATATATCGATGGAGCGAAGAGT
  ATTTTCGTATACGTTTATAGTGCGTCTATTTTACCAGGTATGATTCCTTGGTTTATGATT
  TTATTCAGTCATTTACGTTTTAGAAAATTACACCCTGAAGAATTGGAAGGTCACCCATTC
  AAAATGCCTGGTGGCGCAGTAACTAACTATTTAACAATCTTATTCTTAATATTAGTATTG
  GTTGGTATGGTATTTAATGTTGAAACTAGAATATCAGTGCTTATTGGTGTCATCTTCTTA
  ACGATTGTAACGATTTATTATTTTATTAGATATAATAAAAATAATGTAAAAGCAAAATAG
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⊟Protein

⊟General

• locus tag: NWMN_RS12790 [old locus tag: NWMN_2218 ]
• symbol: NWMN_RS12790
• description: alanine glycine permease
• length: 459
• theoretical pI: 9.70264
• theoretical MW: 50351.8
• GRAVY: 0.870588

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 355.9)
  and 7 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 272.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 113.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 106.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 69.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 41.2)
  histidine-histamine antiporter (TIGR04298; HMM-score: 23.6)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 23.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 343.3)
  and 2 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 124.4)
  no clan defined PrgI; PrgI family protein (PF12666; HMM-score: 16.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007743
  • TAT(Tat/SPI): 0.010608
  • LIPO(Sec/SPII): 0.081992
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 446351819 NCBI
• RefSeq: WP_000429674 NCBI
• UniProt: see NWMN_2218

⊟Protein sequence

• MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVILAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVGMSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTIILMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIGITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKIGITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTVVAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHPEELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIRYNKNNVKAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, CodY see NWMN_2218

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications