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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2309 [new locus tag: SACOL_RS12145 ]
  • pan locus tag?: SAUPAN005796000
  • symbol: SACOL2309
  • pan gene symbol?: —
  • synonym:
  • product: amino acid permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2309 [new locus tag: SACOL_RS12145 ]
  • symbol: SACOL2309
  • product: amino acid permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2371102..2372481
  • length: 1380
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAGAACAATGAACTACAAAGGGGATTGAGTGCCCGTCAAATTCAAATGATTGCACTT
    GGTGGTACGATTGGCGTGGGGCTTTTCATGGGTGCGACAAGTACAATTAAATGGACAGGC
    CCATCAGTTATCCTTGCATATTTAATTGCGGGTATCTTTTTATTTTTAATCATGAGAGCA
    ATGGGGGAAATGATTTATTTAAACCCTACAACAGGATCATTTGCAACATTTGCAAGTGAT
    TATATACATCCTGCAGCAGGTTATATGACAGCATGGAGTAATATATTCCAATGGATTGTA
    GTTGGTATGAGTGAGGTCATCGCAGTAGGAGAATATATGAAGTTTTGGTTCCCGGAATTG
    CCAACTTGGATTCCTGGTGTTATTGCTATTTTATTATTAATGGCAGCGAATTTATTCTCG
    GTAAAAGCGTTTGGAGAATTTGAATTTTGGTTTGCTTTAATTAAAGTTGTAACAATTATT
    TTAATGATTATTGCTGGTTTTGGTCTTATTTTCTTTGGTTTTGGAAATGGTGGCCATGCG
    GTAGGTATTTCTAATCTATGGACAAATGGCGGATTTATGCCAAATGGTATTGTTGGTTTC
    TTCTTTGCATTATCAATTGTAATTGGTTCATACCAAGGTGTGGAACTGATAGGTATTACG
    GCAGGTGAAACTAAAGATCCTCAGAAAAATATCGTTAAAGCAGTGAATGGTGTTATCTGG
    AGAATTTTAATTTTCTATTTAGGCGCTATTTTTGTTATTGTTTCAGTGTATCCTTGGAAT
    CAATTAGGAGACATTGGAAGTCCGTTTGTTGCAACATTTGCTAAAATCGGTATTACATTT
    GCAGCTGGATTAATTAACTTTGTTGTATTAACTGCAGCAATGTCAGGATGTAACTCAGGT
    ATTTTCAGTGCGAGTCGTATGATTTATACACTTGCTCATAAAGGTGAAATGCCGAAAATA
    TTTACTAAAATCATGAGAAATGGTGTACCATTGTACACTGTTGTAGCAGTATCTCTTGGT
    ATTTTAATTGGTGCTTTATTAAACGTAATTTTACCTTTATATATCGATGGAGCGAAGAGT
    ATTTTCGTATACGTTTATAGTGCGTCTATTTTACCAGGTATGATTCCTTGGTTTATGATT
    TTATTCAGTCATTTACGTTTTAGAAAATTACACCCTGAAGAATTGGAAGGTCACCCATTC
    AAAATGCCTGGTGGCGCAGTAACTAACTATTTAACAATCTTATTCTTAATATTAGTATTG
    GTTGGTATGGTATTTAATGTTGAAACTAGAATATCAGTGCTTATTGGTGTCATCTTCTTA
    ACGATTGTAACGATTTATTATTTTATTAGATATAATAAAAATAATGTAAAAGCAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2309 [new locus tag: SACOL_RS12145 ]
  • symbol: SACOL2309
  • description: amino acid permease
  • length: 459
  • theoretical pI: 9.70264
  • theoretical MW: 50351.8
  • GRAVY: 0.870588

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 355.9)
    and 7 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 272.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 113.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 106.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 69.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 41.2)
    histidine-histamine antiporter (TIGR04298; HMM-score: 23.6)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 23.5)
  • TheSEED  :
    • Histidine transport protein (permease)
    Amino Acids and Derivatives Histidine Metabolism Histidine Degradation  Histidine transport protein (permease)
  • PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 343.3)
    and 2 more
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 124.4)
    no clan defined PrgI; PrgI family protein (PF12666; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007743
    • TAT(Tat/SPI): 0.010608
    • LIPO(Sec/SPII): 0.081992
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVILAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVGMSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTIILMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIGITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKIGITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTVVAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHPEELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIRYNKNNVKAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, CodY (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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