PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS12985 [old locus tag: NWMN_2256 ]
• pan locus tag^?: SAUPAN005858000
• symbol: NWMN_RS12985
• pan gene symbol^?: tcaB
• synonym:
• product: Bcr/CflA family drug resistance efflux transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS12985 [old locus tag: NWMN_2256 ]
• symbol: NWMN_RS12985
• product: Bcr/CflA family drug resistance efflux transporter
• replicon: chromosome
• strand: -
• coordinates: 2478180..2479388
• length: 1209
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2478180..2479388) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2256

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAATGATTTAACGCATTTTAACAAGCGTTCACCGATATTTATTATTATTTTAGGATCT
  TTGACGGCCATTGGAGCATTATCGATAGATATGTTTTTGCCTGGATTACCAGATATTAGA
  CATGATTTTCAAACAACGACGTCTAACGCACAATTAACATTATCGATGTTTATGATTGGT
  TTAGCGTTTGGTAATTTGTTTGCTGGTCCAATTTCAGATTCAACTGGGCGCAGAAAGCCG
  CTTATTATAGCTATGATTATTTTTACATTAGCAAGTTTAGGTATTGTTTTTGTACATAAT
  ATTTGGCTGATGGTTGCATTAAGATTTCTTCAAGGAGTGACAGGTGGCGCAGCAGCAGTT
  ATTTCAAGAGCTATAGCAAGTGATATGTATAGTGGTAATGAACTAACTAAGTTTATGGCA
  TTATTGATGCTTGTTAATGGTATTGCACCAGTTGTTGCGCCAACAATAGGGGGCATAATT
  TTAAATTATTCTGTCTGGCGTATGGTCTTTGTCATTCTGACTATCTTTGGCTTCGTCATG
  GTCATAGGTTCTTTATTAAAAGTACCTGAATCATTAACAGTGACAAATCGTGAGTCAAGT
  AGTGGATTAAAGACAATGTTTAAAAACTTCAAAATATTATTGAAGACGCCACGTTTTGTA
  TTACCAATGCTCATTCAAGGTATGACATTTGTAATATTATTTACTTATATTTCTGCGTCT
  CCGTTTATTATTCAAAAAATTTATGGCATGACTGCAATACAATTTAGTTGGATGTTTGCA
  GGAATTGGTATTACGCTCATTATATCAAGTCAACTTACTGGGTACCTTGTTGATTTTATA
  GACTCACAAAAATTGATGAGAGGCATGACGATGATTCAGATTATCGGTGTAATTTTAGTG
  ACGATTGTATTGCTTAATCATTGGAACTTTTGGATTTTAGCGATAGGATTTATCATTTTA
  ATAGCACCAGTGACAGGTGTTGCGACACTTGGTTTTACAATAGCTATGGATGAAAGTAGT
  AGTGGTAGAGGGAGTTCATCAAGTTTATTAGGTTTAGTACAGTTTTTATTTGGTGGTGTT
  GCATCGCCATTAGTGGGGGTAAAAGGTGAAGATAATCCTATACCTTACATAATTATTATT
  ATAGCTACAGCAGTTATATTAATTATTTTACAGATTTATAATATGAAGGTATTTAAAACA
  AATCGATGA
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⊟Protein

⊟General

• locus tag: NWMN_RS12985 [old locus tag: NWMN_2256 ]
• symbol: NWMN_RS12985
• description: Bcr/CflA family drug resistance efflux transporter
• length: 402
• theoretical pI: 10.4555
• theoretical MW: 43822.5
• GRAVY: 0.935572

⊟Function

• TIGRFAM:
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 289.4)
  and 14 more
  multidrug resistance protein (TIGR00880; HMM-score: 92.5)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 69)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 69)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 68.8)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 58.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 57.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 45.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 39.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 30.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 30.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 25.8)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 24.5)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 24.4)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 23.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 149.4)
  and 3 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 50.6)
  MFS_4; Uncharacterised MFS-type transporter YbfB (PF06779; HMM-score: 35.3)
  MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 34)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.128182
  • TAT(Tat/SPI): 0.006508
  • LIPO(Sec/SPII): 0.049011
• predicted transmembrane helices (TMHMM): 11

⊟Accession numbers

• GI: 446921875 NCBI
• RefSeq: WP_000999131 NCBI
• UniProt: see NWMN_2256

⊟Protein sequence

• MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQTTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIWLMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGIILNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPRFVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYLVDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTIAMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQIYNMKVFKTNR

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications