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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13210 [old locus tag: NWMN_2297 ]
  • pan locus tag?: SAUPAN005928000
  • symbol: NWMN_RS13210
  • pan gene symbol?: narG
  • synonym:
  • product: nitrate reductase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS13210 [old locus tag: NWMN_2297 ]
  • symbol: NWMN_RS13210
  • product: nitrate reductase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2521723..2525412
  • length: 3690
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2521723..2525412) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2297

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
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    2401
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    3001
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    3661
    ATGGGAAAATTTGGATTGAATTTCTTTAAGCCAACAGAAAAATTTAATGGGAATTGGTCG
    ATCCTAGAAAGTAAAAGTAGAGAATGGGAAAAAATGTACAGAGAACGTTGGAGCCACGAT
    AAAGAAGTAAGAACAACACATGGTGTTAACTGTACAGGCTCATGTTCTTGGAAAGTATTT
    GTGAAAAATGGTGTGATTACCTGGGAAAATCAACAAACTGACTATCCAAGTTGTGGTCCG
    GATATGCCTGAATATGAACCGAGAGGATGTCCACGAGGTGCGTCATTCTCTTGGTATGAA
    TACAGTCCGCTTCGAATCAAATATCCATATATTCGTGGAAAACTCTGGGATTTATGGACT
    GAAGCATTAGAAGAAAACAATGGTAATCGCGTTGCTGCATGGGCGTCTATTGTTGAAAAT
    GAAGACAAAGCCAAACAATATAAGCAAGCCCGAGGTATGGGAGGGCACGTGCGTTCAAAT
    TGGAAAGACGTTACAGAGATAATCGCAGCACAATTACTGTATACAATAAAAAAATATGGT
    CCAGATCGAATCGCAGGATTTACACCTATTCCAGCGATGTCAATGATTAGTTATGCAGCA
    GGTGCTCGATTCATCAATTTGCTTGGTGGTGAAATGCTTAGTTTTTATGACTGGTATGCA
    GATTTACCACCTGCCTCTCCACAAATTTGGGGAGAGCAAACAGATGTGCCTGAATCAAGT
    GACTGGTATAACGCATCATACATTATTATGTGGGGCTCTAATGTACCTTTAACACGTACT
    CCGGATGCACATTTTATGACTGAAGTCCGCTATAAAGGTACAAAAGTCATTTCAGTAGCA
    CCAGATTACGCAGAAAATGTGAAATTTGCAGATAACTGGCTAGCACCGAATCCTGGTTCA
    GATGCTGCAATTGCACAAGCAATGACGCATGTTATTTTACAAGAACATTATGTTAATCAA
    CCTAATGAACGCTTTATAAATTACGCTAAACAATATACAGATATGCCGTTTCTTATCATG
    CTGGATGAAGATGAAAATGGATATAAAGCGGGTCGATTTTTAAGAGCGAGTGACTTAGGT
    CAAACAACAGAGCAAGGCGAATGGAAGCCAGTTATTCATGATGCAATCAGCGATAGTTTA
    GTAGTACCTAATGGCACAATGGGTCAACGTTGGGAAGAAGGTAAGAAGTGGAACTTAAAA
    CTAGAAACAGAAGATGGTTCTAAAATTAACCCTACATTATCAATGACAGAAGGTGGATAC
    GAATTAGAAACAATTCAATTCCCATACTTTGATAGTGATGGAGATGGGATATTCAATCGT
    CCAATTCCAACTCGACAAGTCACTTTAGCAAATGGTGACAAAGTCCGTATTGCTACAATT
    TTTGACTTAATGGCAAGTCAATATGGCGTGCGTCGTTTTGATCATAAATTAGAATCAAAA
    GGATACGACGATGCAGAATCAAAATATACACCTGCTTGGCAAGAAGCCATTTCAGGCGTA
    AAACAAAGTGTTGTCATTCAAGTAGCGAAAGAATTTGCGCAAAACGCTATCGATACTGAA
    GGGCGTTCAATGATTATCATGGGTGCGGGTATTAACCATTGGTTTAACTCAGATACGATT
    TATCGTTCAATCTTAAACTTAGTTATGTTATGTGGCTGTCAAGGTGTGAATGGTGGCGGT
    TGGGCTCACTATGTGGGACAAGAAAAATGTCGTCCGATTGAAGGATGGAGTACTGTCGCA
    TTTGCGAAAGACTGGCAAGGACCACCACGTTTGCAAAACGGAACAAGTTGGTTCTATTTT
    GCAACAGACCAATGGAAATATGAAGAGTCAAATGTAGATCGTTTAAAATCTCCATTAGCT
    AAAACAGAGGAGTTAAAGCATCAACATCCAGCTGATTATAATGTTTTAGCAGCTAGACTT
    GGTTGGTTACCATCATATCCACAATTTAATAAAAATAGTTTGTTGTTTGCAGAAGAAGCT
    AAAGATGAAGGTATAGATTCAAATGAAGCGATTTTGCAACGTGCAATTGATGAAGTGAAA
    TCAAAACAAACACAATTTGCAATAGAAGATCCTGATTTGAAAAAGAATCATCCAAAATCA
    TTATTTATATGGCGTTCAAACTTAATTTCAAGTTCTGCAAAAGGTCAAGAATACTTTATG
    AAGCATTTACTTGGCACAAAATCAGGGTTATTAGCTACACCAAATGAAGATGAAAAGCCA
    GAAGAAATTACGTGGCGTGAAGAAACAACAGGTAAGTTAGATTTAGTGGTCTCTTTAGAC
    TTCAGAATGACGGCAACACCGTTATATTCTGACATTGTTTTGCCGGCAGCGACTTGGTAT
    GAAAAACATGATTTATCATCAACGGATATGCATCCATATGTACATCCTTTCAATCCTGCC
    ATTGATCCGTTATGGGAATCGCGTTCAGACTGGGATATTTATAAAACGTTGGCAAAAGCA
    TTTTCAGAAATGGCAAAAGACTATTTACCTGGAACGTTTAAAGATGTTGTGACAACTCCA
    CTTAGTCATGATACAAAGCAAGAAATTTCAACACCATACGGCGTAGTGAAAGATTGGTCG
    AAGGGTGAAATTGAAGCGGTACCTGGACGTACAATGCCTAACTTTGCAATTGTAGAACGC
    GACTACACTAAAATTTACGACAAATATGTCACGCTTGGTCCTGTACTTGAAAAAGGGAAA
    GTTGGAGCACATGGTGTAAGTTTCGGTGTCAGTGAACAATATGAAGAATTAAAAAGTATG
    TTAGGTACGTGGAGTGATACAAATGATGATTCTGTGAGAGCGAATCGTCCACGTATTGAT
    ACAGCACGTAATGTAGCTGATGCAATACTAAGTATTTCATCTGCTACGAATGGTAAATTA
    TCACAAAAATCATATGAAGATCTCGAAGAACAAACTGGAATGCCGTTAAAAGATATTTCT
    AGCGAACGCGCTGCTGAGAAAATTTCGTTTTTAAATATAACTTCACAACCACGAGAAGTA
    ATACCGACAGCAGTATTCCCAGGTTCAAATAAACAAGGTCGACGATATTCACCATTTACA
    ACGAATATAGAACGTCTAGTACCTTTTAGAACATTAACAGGACGTCAAAGTTATTATGTG
    GATCACGAAGTTTTCCAACAATTTGGGGAGAGCTTACCAGTATATAAACCGACATTGCCG
    CCAATGGTATTTGGGAATAGAGATAAGAAAATTAAAGGTGGTACAGATGCTTTGGTACTG
    CGTTATTTAACGCCTCATGGAAAATGGAATATACACTCAATGTATCAAGATAATAAGCAT
    ATGTTGACACTATTTAGAGGTGGTCCAACGGTTTGGATATCAAATGAAGATGCTGAAAAA
    CACGATATCCAAGATAATGATTGGCTAGAAGTGTATAACCGTAATGGTGTTGTAACGGCA
    AGAGCAGTTATTTCGCATCGTATGCCTAAAGGTACAATGTTTATGTATCATGCACAAGAT
    AAACATATTCAAACGCCTGGGTCAGAAATTACAGATACACGTGGTGGTTCACACAACGCG
    CCGACTAGAATCCATTTGAAACCAACACAACTAGTCGGAGGATACGCACAAATTAGTTAT
    CACTTTAATTATTATGGACCAATTGGGAACCAAAGGGATTTATATGTAGCAGTTAGAAAG
    ATGAAGGAGGTTAATTGGCTTGAAGATTAA
    60
    120
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    2160
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    3420
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    3690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS13210 [old locus tag: NWMN_2297 ]
  • symbol: NWMN_RS13210
  • description: nitrate reductase subunit alpha
  • length: 1229
  • theoretical pI: 6.19853
  • theoretical MW: 139828
  • GRAVY: -0.592596

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic nitrate reductase, alpha subunit (TIGR01580; EC 1.7.99.4; HMM-score: 1838.2)
    and 13 more
    DMSO reductase family type II enzyme, molybdopterin subunit (TIGR03479; HMM-score: 591.5)
    anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family (TIGR02166; HMM-score: 180.3)
    formate dehydrogenase, alpha subunit (TIGR01591; EC 1.2.1.2; HMM-score: 132.8)
    molybdopterin guanine dinucleotide-containing S/N-oxide reductases (TIGR00509; HMM-score: 102.5)
    trimethylamine-N-oxide reductase TorA (TIGR02164; EC 1.7.2.3; HMM-score: 78.6)
    Metabolism Energy metabolism Anaerobic formate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.7)
    Metabolism Energy metabolism Electron transport formate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.7)
    Metabolism Energy metabolism Electron transport arsenite oxidase, large subunit (TIGR02693; EC 1.20.9.1; HMM-score: 28.3)
    Metabolism Central intermediary metabolism Nitrogen metabolism periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    Metabolism Energy metabolism Electron transport periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    Metabolism Energy metabolism Aerobic periplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.6)
    oxidoreductase alpha (molybdopterin) subunit (TIGR01701; HMM-score: 19.7)
    Metabolism Energy metabolism Electron transport NADH dehydrogenase (quinone), G subunit (TIGR01973; EC 1.6.99.5; HMM-score: 13.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Molybdopterin; Molybdopterin oxidoreductase (PF00384; HMM-score: 539.6)
    and 3 more
    AcetylDC-like (CL0332) Molydop_binding; Molydopterin dinucleotide binding domain (PF01568; HMM-score: 75.9)
    4Fe-4S (CL0344) Nitr_red_alph_N; Respiratory nitrate reductase alpha N-terminal (PF14710; HMM-score: 38.9)
    Molybdop_Fe4S4; Molybdopterin oxidoreductase Fe4S4 domain (PF04879; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mo-bis(molybdopterin guanine dinucleotide), [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.069378
    • TAT(Tat/SPI): 0.00154
    • LIPO(Sec/SPII): 0.003017
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVRTTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYSPLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSNWKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDWYADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKVISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTDMPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWEEGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLANGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQVAKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVGQEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEELKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSKQTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKPEEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFRTLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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