From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13545 [old locus tag: NWMN_2350 ]
  • pan locus tag?: SAUPAN006007000
  • symbol: NWMN_RS13545
  • pan gene symbol?: pnbA
  • synonym:
  • product: carboxylesterase/lipase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS13545 [old locus tag: NWMN_2350 ]
  • symbol: NWMN_RS13545
  • product: carboxylesterase/lipase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2585758..2587110
  • length: 1353
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2585758..2587110) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2350

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGATTAATACTACAGGTGGTCAAATTCATGGTATTACACAAGATGGTTTAGATATC
    TTCTTAGGCATTCCTTATGCAGAACCACCAGTTCATGACAATCGCTTTAAACATTCTACG
    TTAAAAACACAATGGTCAGAGCCAATTGATGCAACTGAAATACAACCCATCCCACCGCAA
    CCAGACAACAAATTAGAAGATTTTTTCTCCTCACAATCTACAACTTTTACTGAACATGAA
    GACTGTTTATATCTAAATATTTGGAAACAACATAATGATCAGACGAAGAAACCTGTCATC
    ATTTATTTTTATGGTGGTAGTTTTGAAAATGGTCATGGTAAAGCCGAACTCTATCAACCG
    GCACATTTAGTACAAAATAACGACATTATCGTTATTACATGCAATTATCGTTTAGGCGCA
    TTAGGATATTTAGACTGGTCATATTTTAATAAAGATTTTCATTCCAATAATGGCCTTTCA
    GATCAAATCAATGTCATAAAATGGGTGCATCAATTTATTGAATCCTTCGGTGGCGACGCT
    AATAACATTACTTTAATGGGTCAGTCTGCAGGCAGTATGAGCATTTTGACTTTACTTAAA
    ATACCTGACATTGAGCCATACTTCCATAAAGTCGTTCTACTAAGTGGCGCACTACGATTA
    GACACCCTTGAGAGTGCACGCAATAAAGCACAACATTTCCAAAAAATGATGCTCGATTAT
    TTAGATACAGATGATGTTACATCATTATCGACAAATGATATTCTTATGCTGATGGCGAAG
    CTAAAACAATCTCGAGGACCTTCTAAAGGGCTTGATTTAATATATGCGCCTATTAAAACA
    GATTATATACAAAATAATTATCCAACAACGAAACCAATTTTTGCATGTTATACAAAAGAT
    GAAGGCGATATTTATATTACTAGTGAACAGAAAAAATTATCGCCGCAACGCTTTATCGAC
    ATTATGGAATTAAATGATATTCCTTTAAAATACGAAGATGTTCAGACGGCGAAGCAACAA
    TCTTTAGCGATTACACATTGTTATTTCAAACAGCCGATGAAGCAATTTTTACAACAACTC
    AATATACAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCT
    AGCGCACACTATCGCAGTGCTTACCATATTCTAGATATGGTATTTTGGTTCGGAAACTTA
    CAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAA
    ATGCAAAATGATTTAGCTAATTTTGCTAAAAGCGGTAAAATGCCTTGGCCGATGTATCAT
    AATGAACGTCGCTATTATCGCACATATCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1353

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS13545 [old locus tag: NWMN_2350 ]
  • symbol: NWMN_RS13545
  • description: carboxylesterase/lipase family protein
  • length: 450
  • theoretical pI: 6.60146
  • theoretical MW: 51995.6
  • GRAVY: -0.468222

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    AB_hydrolase (CL0028) COesterase; Carboxylesterase family (PF00135; HMM-score: 253.7)
    and 4 more
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 27.2)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 20.5)
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 18.5)
    SGNH_hydrolase (CL0264) ALGX; SGNH hydrolase-like domain, acetyltransferase AlgX (PF16822; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01266
    • TAT(Tat/SPI): 0.001294
    • LIPO(Sec/SPII): 0.002138
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQWSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIYFYGGSFENGHGKAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLSDQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGALRLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYAPIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDVQTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILDMVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS13545&oldid=91964"