PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS13860 [old locus tag: NWMN_2410 ]
• pan locus tag^?: SAUPAN006148000
• symbol: NWMN_RS13860
• pan gene symbol^?: —
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS13860 [old locus tag: NWMN_2410 ]
• symbol: NWMN_RS13860
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2653437..2654600
• length: 1164
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2653437..2654600) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2410

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1141
  
  ATGATTAATAGTAATATCAGATTATATATTCTAGGATTTTTAGCATTTTTCGCATCATTG
  ATTCAAAACATATACACGCCTATTATTCCGAGACTATATGACGATTTTCAAGTACCACTA
  TTTTGGATAAATGCGACAGTTGGCGGTTTTATATTTATCGTTGCAGTTATGCAAATTGTA
  CTTGGTAGAAGTATAGATTCTCGTGATTCGAAAAAAGTACTTTTAACGGGATTGGGAATA
  GTAATCATATCTAGCTTTATTTGTGCAGTGACACATAACTTTATATTATTTGCTATATCA
  AGGTTATTTCAAGCAATTGGTTGCGGAATTATTCCTCTCGTGACTTTAACTTTATTAGCC
  AAACTAAGTACAGATAATGGTAGAGCTCAAGCTATGGCGAACTATCAAATATTTTTATCA
  TGTGCACCAGCTTTGGCACCAATCTTAGGGAGTACTTTAGGTGCAAGATGGGATTATATT
  GGTATTTTTAGTTTTTTGCTCGTTATATCTATCGTATTATTTTTGATTATCTTTTTTATT
  GATATTCCAAATGTTGAAAAAGGGATTGTAAAATTAACTGAAAAAATTGAAGAAAAATAT
  TTGACCGATAAAGTGTTTATTACTTTAGTGACATTGGGATTTTTAATATTTTTTACGTAC
  TTTTCAATATTAGTTTATTTACCAACATTATTAAATAATACCTATGATATAGGCGTTGGA
  ATATCAGGAGTTTTGTTTTTACCTATTACTGTTAGTGTTATATTAGGAAGTTTATTTTAT
  AAGCGATTTTCAAAAAAATATAATGAAATAATGATACTTCGTGTTACTATAACTGGATTT
  GCTATATTTACGTTATTGTTTGGATGGTTAAACGAATCAAATGTAATTATATTATCAGTA
  ATTATATTTATATTAGGTACTTGTGTTGGTATTGTGCCAGCTTTGCTGTCTACTATAATT
  TCTAAAAGGTTTGAACATATAAAAGGGAAAGTGCTAGGTGTATTTAATTTTGTGAGATAT
  ATTGGAATGACTGTCGGTGCATTATTAATTGGTATCATTTCTCAGCCGTTGGTAGCCTTT
  TACTTCACAACTATAACTATCATGTTAATAGTAATATTTCTTTATATAAAGATAGTTGAC
  TTTCAGCTAAAGTATGCCAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS13860 [old locus tag: NWMN_2410 ]
• symbol: NWMN_RS13860
• description: MFS transporter
• length: 387
• theoretical pI: 9.77147
• theoretical MW: 43229.9
• GRAVY: 1.11705

⊟Function

• TIGRFAM:
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 101.2)
  and 4 more
  multidrug resistance protein (TIGR00880; HMM-score: 70.1)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 28.7)
• TheSEED: data available for COL, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 147.8)
  and 3 more
  MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 20.3)
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 7)
  no clan defined DUF4220; Domain of unknown function (DUF4220) (PF13968; HMM-score: 5.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.027152
  • TAT(Tat/SPI): 0.000354
  • LIPO(Sec/SPII): 0.064259
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446533538 NCBI
• RefSeq: WP_000610884 NCBI
• UniProt: see NWMN_2410

⊟Protein sequence

• MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWINATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRLFQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYIGIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFFTYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications