PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
• pan locus tag^?: SAUPAN006375000
• symbol: NWMN_RS14610
• pan gene symbol^?: —
• synonym:
• product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
• symbol: NWMN_RS14610
• product: N-acetylmuramoyl-L-alanine amidase
• replicon: chromosome
• strand: +
• coordinates: 2801862..2803721
• length: 1860
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2801862..2803721) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2543

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCCTAAAAATAAAATTTTAATTTATTTGCTATCAACTACGCTCGTATTACCTACTTTA
  GTTTCACCTACCGCTTATGCTGATACACCTCAAAAAGATACTACAGCTAAGACAACATCT
  CATGATTCAAAAAAATCTAATGACGATGAAACTTCTAAGGATACTACAAGTAAAGATATT
  GATAAAGCAGACAAAAATAATACAAGTAACCAAGACAATAACGACAAAAAATTTAAAACT
  ATAGACGACAGCACTTCAGACTCTAACAATATCATTGATTTTATTTATAAGAATTTACCA
  CAAACCAATATAAACCAATTGCTAACCAAAAATAAATACGATGATAATTACTCATTAACA
  ACTTTAATCCAAAACTTATTCAATTTAAATTCGGATATTTCTGATTACGAACAACCTCGT
  AATGGCGAAAAGTCAACAAATGATTCGAATAAAAACAGTGACAATAGCATCAAAAATGAC
  ACTGATACGCAATCATCTAAACAAGATAAAGCAGACAATCAAAAAGCACCTAAATCAAAC
  AATACAAAACCAAGTACATCTAATAAGCAACCAAATTCGCCAAAGCCAACACAACCTAAT
  CAATCAAATAGTCAACCAGCAAGTGACGATAAAGCAAATCAAAAATCTTCATCGAAAGAT
  AATCAATCAATGTCAGATTCGGCTTTAGACTCTATTTTGGATCAATACAGTGAAGATGCA
  AAGAAAACACAAAAAGATTATGCATCTCAATCTAAAAAAGACAAAAATGAAAAATCTAAT
  ACAAAGAATCCACAGTTACCAACACAAGATGAATTGAAACATAAATCTAAACCTGCTCAA
  TCATTCAATAACGATGTTAATCAAAAGGATACACGTGCAACATCATTATTCGAAACAGAT
  CCTAGTATATCTAACAATGATGATAGCGGACAATTTAACGTTGTTGACTCAAAAGATACA
  CGTCAATTTGTCAAATCAATTGCTAAAGATGCACATCGCATTGGTCAAGATAACGATATT
  TATGCGTCTGTCATGATTGCCCAAGCAATCTTAGAATCTGACTCAGGTCGTAGTGCTTTA
  GCTAAGTCACCAAACCATAATTTATTCGGTATCAAAGGTGCTTTTGAAGGGAATTCTGTT
  CCTTTTAACACATTAGAAGCTGATGGTAATCAATTGTATAGTATTAATGCTGGATTCCGA
  AAATATCCAAGCACGAAAGAATCACTAAAAGATTACTCTGACCTTATTAAAAATGGTATT
  GATGGCAATCGAACAATTTATAAACCAACATGGAAATCGGAAGCCGATTCTTATAAAGAT
  GCAACATCACACTTATCTAAAACATATGCTACAGATCCAAACTATGCTAAGAAATTAAAC
  AGTATTATTAAACACTATCAATTAACTCAGTTTGACGATGAACGCATGCCAGATTTAGAT
  AAATATGAACGTTCTATCAAGGATTATGATGATTCATCAGATGAATTCAAACCTTTCCGT
  GAGGTATCTGATAGTATGCCATATCCACATGGCCAATGTACTTGGTACGTATATAACCGT
  ATGAAACAATTTGGTACATCTATCTCAGGTGATTTAGGTGATGCACATAATTGGAATAAT
  CGAGCTCAATACCGTGATTATCAAGTAAGTCATACACCAAAACGTCATGCTGCTGTTGTA
  TTTGAGGCTGGACAATTTGGTGCAGATCAACATTACGGTCATGTAGCATTTGTTGAAAAA
  GTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATT
  TCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAA
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⊟Protein

• 5T1Q
  PDB

Protein Data Bank: 5T1Q

⊟General

• locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
• symbol: NWMN_RS14610
• description: N-acetylmuramoyl-L-alanine amidase
• length: 619
• theoretical pI: 6.34359
• theoretical MW: 69252.8
• GRAVY: -1.13279

⊟Function

• TIGRFAM:
  flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 81.9)
  and 1 more
  pentapeptide MXKDX repeat protein (TIGR02953; HMM-score: 15.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 75.2)
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 66.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.99354
  • TAT(Tat/SPI): 0.004521
  • LIPO(Sec/SPII): 0.001352
  • Cleavage Site: CS pos: 27-28. AYA-DT. Pr: 0.9885
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447048355 NCBI
• RefSeq: WP_001125611 NCBI
• UniProt: see NWMN_2543

⊟Protein sequence

• MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications