PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS14620 [old locus tag: NWMN_2545 ]
• pan locus tag^?: SAUPAN006379000
• symbol: NWMN_RS14620
• pan gene symbol^?: sasF
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS14620 [old locus tag: NWMN_2545 ]
• symbol: NWMN_RS14620
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2804716..2806623
• length: 1908
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2804716..2806623) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2545

⊟Phenotype

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⊟DNA sequence

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  ATGGCTAAATATCGAGGGAAACCGTTTCAATTATATGTAAAGTTATCGTGTTCGACAATG
  ATGGCGACAAGTATCATTTTAACGAATATCTTGCCGTACGATGCCCAAGCTGCATCTGAA
  AAGGATACTGAAATTACAAAAGAGATATTATCTAAGCAAGATTTATTAGACAAAGTTGAC
  AAGGCAATTCGTCAAATTGAGCAATTAAAACAGTTATCGGCTTCATCTAAAGAACATTAT
  AAAGCACAACTAAATGAAGCGAAAACAGCATCGCAAATAGATGAAATCATAAAACGAGCT
  AATGAGTTGGATAGCAAAGACAATAAAAGTTCTCACACTGAAATGAACGGTCAAAGTGAT
  ATAGACAGTAAATTAGATCAATTGCTTAAAGATTTAAATGAGGTTTCTTCAAATGTTGAT
  AGGGGTCAACAAAGTGGCGAGGACGATCTTAATGCAATGAAAAATGATATGTCACAAACG
  GCTACAACAAAACATGGAGAAAAAGATGATAAAAATGATGAAGCAATGGTAAATAAGGGG
  TTAGAAGACCTAGACCATTTGAATCAGCAAATACACAAATCGAAAGATGCATCGAAAGAT
  ACATCGGAAGATCCAGCAGTGTCTACAACAGATAATAATCATGAAGTAGCTAAAACGCCA
  AATAATGATGGTTCTGGACATGTTGTGTTAAATAAATTCCTTTCAAATGAAGAGAATCAA
  AGCCATAGTAATCGACTCACTGATAAATTACAAGGAAGCGATAAAATTAATCATGCTATG
  ATTGAAAAATTAGCTAAAAGTAATGCCTCAACGCAACATTACACATATCATAAACTGAAT
  ACGTTACAATCTTTAGATCAACGTATTGCAAATACGCAACTTCCTAAAAATCAAAAATCA
  GACTTAATGAGCGAAGTAAATAAGACGAAAGAGCGTATAAAAAGTCAACGAAATATTATT
  TTGGAAGAACTTGCACGTACTGATGATAAAAAGTATGCTACACAAAGCATTTTAGAAAGT
  ATATTTAATAAAGACGAGGCAGTTAAAATTCTAAAAGATATACGTGTTGATGGTAAAACA
  GATCAACAAATTGCAGATCAAATTACTCGTCATATTGATCAATTATCTCTGACAACGAGT
  GATGATTTATTAACGTCATTGATTGATCAATCACAAGATAAGTCGCTATTGATTTCTCAA
  ATTTTACAAACGAAATTAGGAAAAGCTGAAGCAGATAAATTGGCTAAAGATTGGACGAAT
  AAAGGATTATCAAATCGCCAAATCGTTGACCAATTGAAGAAACATTTTGCATCAACTGGC
  GACACGTCTTCAGATGATATATTAAAAGCAATTTTGAATAATGCCAAAGATAAAAAACAA
  GCAATTGAAACGATTTTAGCAACACGTATAGAAAGACAAAAGGCAAAATTACTGGCAGAT
  TTAATTACTAAAATAGAAACAGATCAAAATAAAATTTTTAATTTAGTTAAATCGGCATTG
  AATGGTAAAGCGGATGATTTATTGAATTTACAAAAGAGACTCAATCAAACGAAAAAAGAT
  ATAGATTATATTTTATCACCAATAGTAAATCGTCCAAGTTTACTAGATCGATTGAATAAA
  AATGGGAAAACGACAGATTTAAATAAGTTAGCAAATTTAATGAATCAAGGATCAGATTTA
  TTAGACAGTATTCCAGATATACCCACACCAAAGCCAGAAAAGACGTTAACACTTGGTAAA
  GGTAATGGATTGTTAAGTGGATTATTAAATGCTGATGGTAATGTATCTTTGCCTAAAGCG
  GGGGAAACGATAAAAGAACATTGGTTGCCGATATCTGTAATTGTTGGTGCAATGGGTGTA
  CTAATGATTTGGTTATCACGACGCAATAAGTTGAAAAATAAAGCATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS14620 [old locus tag: NWMN_2545 ]
• symbol: NWMN_RS14620
• description: hypothetical protein
• length: 635
• theoretical pI: 8.35733
• theoretical MW: 70917.6
• GRAVY: -0.752913

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  B_GA (CL0598) GA; GA module (PF01468; HMM-score: 16.2)
  HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 14.7)
  no clan defined Poly_export; Polysaccharide biosynthesis/export protein (PF02563; HMM-score: 13.4)
  and 3 more
  COX5A; Cytochrome c oxidase subunit Va (PF02284; HMM-score: 10.1)
  B2; RNA binding protein B2 (PF11473; HMM-score: 9.7)
  SAP30_Sin3_bdg; Sin3 binding region of histone deacetylase complex subunit SAP30 (PF13867; HMM-score: 8.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 37 aa
  • SP(Sec/SPI): 0.935075
  • TAT(Tat/SPI): 0.01494
  • LIPO(Sec/SPII): 0.003542
  • Cleavage Site: CS pos: 37-38. AQA-AS. Pr: 0.8917
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 447074646 NCBI
• RefSeq: WP_001151902 NCBI
• UniProt: see NWMN_2545

⊟Protein sequence

• MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTEITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANELDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQTATTKHGEKDDKNDEAMVNKGLEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAKTPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYATQSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPDIPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLKNKA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications