From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0097 [new locus tag: SA_RS00645 ]
  • pan locus tag?: SAUPAN000891000
  • symbol: SA0097
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0097 [new locus tag: SA_RS00645 ]
  • symbol: SA0097
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 108046..110283
  • length: 2238
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAACGTGATTATTTAATTCGAGTAGAAACTGAAAGCATGCCAGATTTCAAAAGGCTC
    AATGGTTTAATGATTGGTTTTGTTATTAAAGGTGAGGCACATATTTATGATGAAAATAAC
    ATGACGCAATGCAACAGTGGCGACATTTTCATCATTAACCACCGTGACTTGTATCGATTT
    CAACTTCAACAAGATGGCATCATATGTTATATCCAATTCCAAATGAAATATTTGGCAGAC
    AAGTTTGATGATGCGCATTGTCTATATTTTCACTTAACAGATGCGACCACAACCAAGAAT
    ATACATCAACTGAGAAATATAATGGCAAGACTGGTTTCAACACACATTCGACATAATGAG
    TTGTCTAAATTGACTGAGCAACAACTTGTGATTCAGTTGCTTATGCATATGATTCATTAT
    GTCCCGCGTACATATCATTCGAACCAAAGTATCTTAAATGATGATAAAGTGAATCAAGTA
    TGCGACTATATCGAGTTACATTTTCATGAAGATTTAAGCCTTTCAGAATTAAGCGAATAC
    GTTGGGTGGTCAGAGAGCCATCTGTCTAAAAAGTTTACAGAATCGCTAGGTGTAGGATTC
    CAACATTTCTTAAATACGACGCGAATTGAGCATGCGAAACTCGATTTAACATACACAGAT
    GAAACGATTACTGATATTGCATTGCAAAATGGCTTTTCAAGTGCAGCGAGCTTTGCGAGA
    ACATTTAAACACTTTACGCATCAAACGCCTAAACAATATCGAGGTGATCGTCCAGCAATC
    ACTGAAAATCAACAATCGGCACAACATAATTATCACGACCGTGAATTGATATTACTTTTA
    AATGACTACATTGAAGAAATGAATCATTTCATTGAAGATATTGAAAAGATGAACTATAAA
    GAGATTGCCTTTAAACCAACTAATCAACAACTAAATCAATTTAATCATATTATTCAAGTG
    GGCTATTTGAGGAATTTGCTCAATACACAGTATCAATCACAGTTGCTTACATGTCATCAT
    GATTTTCAAGTCAATGAAGTATTAGCATATGATGTGATGCCATATATTATGAAAAAGCTC
    AATGCGCCATTCACGTATGATGCAGAGATTTCGAATATATTTTATGATATCGATTTGTGT
    TTAGACTTTTTATTAGATCATAACTTTAGTCTAACCATGCATTTGAATCAGTATGACTCA
    CGAGATTATATCGATGCATTCAAAGTATTTATCCATCACGTTGCCCTGCATGTCAGTCAT
    AGAAAAGATTTGAAGTTCAACTTGTATGTGACGACATTGCACACGTCTTTAATTGAAATG
    ATTGATTATTTTAAAGCATTATTCCCTAATGGTGGCTTGTACATTCACTTAGATCAAGCT
    ACGGAAAGACATCTACCATTGTTGAAACGACTTGAGCCACACATCAACCATTTTGTATTT
    GATGCCAATTCAAATGATGCTGTTGATTTTAATAAAATGAATGATGATGAATTTAAAACC
    GCGAGTCAAATGATTATTAATAAAACGAATTACCTTATCGACTTAATGCATCGCCATAAC
    CTAAAGCGTCCACTCATTTTACTCAATTGGAATACATTGACGGGTGATACATTTATTACA
    AACGGCGAATATTTTAGAGGTGGTATCATCATTGAGCAATTATTAAAATTAAGTACTAAA
    GTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAGAAATGAA
    CGGGATTATATGAATTCTATTGAACTGTTTCATCAATATAATGGAAAACGTCCGGTCTAT
    TTCACGGCATTGCTATTTAATAAATTAACAAGCAATATTTTGTATTCTGATGATACATGT
    ATTGTCACGGGAACTGATTCAAATTTTCAAATATTGTTATATGATGCAAAGCATTTTAAT
    CCGTACTTAGCGTTGGACAATCAAATGAATATGCGTGCAACGGAAATGATCCATTTGAAC
    ATTAATGCCCTAGAAGACGGTATGTATAAGATTAAACATTTTACCTTAGATAAAGAAAAT
    GGTGCGTTATTTAATCTTTGGCGCAAACATCATACGATACATGGCATGGACAAGGACTCT
    ATAGATTACGTTAATCGAATGAGTTTTCCGAAATTAGAAGTATATGATATAGATATCACG
    GACACACTGGCATTAAACATTAAAATGATTACGAATGGGATTCACTTAATTGAAGTAAAA
    CGTTACCCAAGTTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2238

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0097 [new locus tag: SA_RS00645 ]
  • symbol: SA0097
  • description: hypothetical protein
  • length: 745
  • theoretical pI: 6.43379
  • theoretical MW: 87877.4
  • GRAVY: -0.372081

Function[edit | edit source]

  • TIGRFAM:
    YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 60.2)
    and 1 more
    4-hydroxyphenylacetate catabolism regulatory protein HpaA (TIGR02297; HMM-score: 45.2)
  • TheSEED  :
    • Transcriptional regulator, AraC family
  • PFAM:
    HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 77.1)
    and 2 more
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 39.1)
    Cupin (CL0029) AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 16.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009528
    • TAT(Tat/SPI): 0.000812
    • LIPO(Sec/SPII): 0.001792
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQCNSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIHQLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQVCDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTYTDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDRELILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQLLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLTMHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPNGGLYIHLDQATERHLPLLKRLEPHINHFVFDANSNDAVDFNKMNDDEFKTASQMIINKTNYLIDLMHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSTKVEGIGYWLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTGTDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGALFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVKRYPSS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]