From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0104 [new locus tag: SA_RS00675 ]
  • pan locus tag?: SAUPAN000904000
  • symbol: SA0104
  • pan gene symbol?: norG
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0104 [new locus tag: SA_RS00675 ]
  • symbol: SA0104
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 118475..119803
  • length: 1329
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGATACCTCCACAGAGGCAGTTGGCGATACAATACAACGTAAATAGAGTGACGATT
    ATTAAAAGTATTGAGTTATTAGAGGCTGAAGGATTTATCTATACTAAAGTGGGGAGTGGA
    ACATATGTTAATGACTATTTGAATGAAGCACATATTACAAATAAGTGGTCTGAAATGATG
    TTATGGTCCTCTCAACAAAGAAGTCAGTATACGGTGCAATTAATTAATAAAATTGAGACA
    GATGATTCGTATATACATATAAGTAAAGGTGAATTGGGTATATCGTTAATGCCACATATT
    CAATTGAAAAAAGCCATGTCTAATACAGCCAGTCATATTGAAGACTTATCTTTTGGTTAT
    AATAATGGCTATGGTTATATCAAGTTAAGAGATATTATCGTTGAACGAATGTCAAAGCAA
    GGTATAAATGTAGGTAGAGAAAATGTAATGATCACTTCAGGCGCTTTACATGCCATTCAA
    CTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATAT
    ATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAAT
    CAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATT
    TATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAA
    AATATAATTACTTATAGCGAAAGACATAATATTCCTATCATTGAAGATGATATCTTTAGA
    GATATTTTCTTTAGCGATCCAACTCCTGCTATCAAAACTTATGATAAATTGGGAAAAGTT
    ATACATATAAGCAGTTTTTCAAAAACGATTGCACCAGCAATAAGAATAGGTTGGATTGTT
    GCTTCTGAAAAAATAATAGAGCAATTGGCAGATGTAAGAATGCAAATTGACTATGGATCC
    AGTATTTTGTCACAAATGGTTGTATATGAGATGTTGAAAAATAAGTCTTATGATAAACAC
    TTAGTAAAGTTAAGGTATGTTTTAAAAGATAAACGAGACTTTATGTTAAACATCCTCAAT
    AATTTATTTAAGGATATAGCACATTGGGAGGTTCCAAGTGGAGGTTATTTTGTATGGTTA
    GTCTTTAAAATAGATATAGATATTAAATATTTATTTTACGAATTGTTAAGTAAAGAAAAA
    ATATTAATCAATCCGGGTTACATTTATGGCAGTAAAGAAAAGAGTATAAGGCTATCTTTT
    GCCTTTGAATCAAATGAAAATATTAAGCATGCGCTCTATAAAATTTATACATATGTGAAA
    AAGGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1329

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0104 [new locus tag: SA_RS00675 ]
  • symbol: SA0104
  • description: hypothetical protein
  • length: 442
  • theoretical pI: 9.43613
  • theoretical MW: 51286.9
  • GRAVY: -0.197059

⊟Function[edit | edit source]

  • TIGRFAM:
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 137.5)
    and 19 more
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 86)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 83.5)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 82.4)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 75.8)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 68)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 62)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 57.5)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 53)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 48.2)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 45.1)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 42.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 42.1)
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 27)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 27)
    Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 26.3)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 23)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 23)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 22.1)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 14.2)
  • TheSEED  :
    • Aspartate aminotransferase (EC 2.6.1.1)
    • Transcriptional regulator, GntR family domain
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Aspartate aminotransferase (EC 2.6.1.1)
    and 2 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Aspartate aminotransferase (EC 2.6.1.1)
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Aspartate aminotransferase (EC 2.6.1.1)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 120.5)
    and 4 more
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 33.8)
    PLP_aminotran (CL0061) Aminotran_MocR; Alanine-glyoxylate amino-transferase (PF12897; HMM-score: 23)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 16.7)
    no clan defined DUF2953; Protein of unknown function (DUF2953) (PF11167; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • gene regulated by NorG*, TF important in Metabolite transportRegPrecise
    SA0103

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006961
    • TAT(Tat/SPI): 0.000303
    • LIPO(Sec/SPII): 0.00055
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIPPQRQLAIQYNVNRVTIIKSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIETDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERMSKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHIDVPYNQINEINTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPIIEDDIFRDIFFSDPTPAIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLADVRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHWEVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNENIKHALYKIYTYVKKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA0104&oldid=90437"