PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0191 [new locus tag: SA_RS01135 ]
• pan locus tag^?: SAUPAN001044000
• symbol: SA0191
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0191 [new locus tag: SA_RS01135 ]
• symbol: SA0191
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 226088..227200
• length: 1113
• essential: no DEG

⊟Accession numbers

• Gene ID: 1122967 NCBI
• RefSeq: NP_373434 NCBI
• BioCyc: see SA_RS01135
• MicrobesOnline: 102460 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  TTGAGGCGTAAAAGGAAATGGCTAATTGTTATTTGTGTATTATTAGTATTGATTACGATA
  TCACAAATTTTGAAACATTTTAACTATGGAACTGAGAGTAAAGAAGCATACAATACATAT
  ACGGTTGAAAAAGAAAGACAATTAGATTTACAAGGTAAGGCATATCCACGTGAAGTTAAA
  ACATATTATAAAAATAATCAGGTTGGAACATATTTGGGTGTGCAAGTAGCGGACGGGCAA
  ACAGTCAAAAAAGGAGACATGTTAATTAATTATAAAATAAATAGTAATAAACGACAGCAA
  CTTGTTGATAAGATAAATGATACTCAACATATTATTACAGAAGTTTATTTAAACATTACT
  AAAGGTTTAAACAATCGAGAGATACAGAAGAAGTTAATTGAATATAAGCAGACATTATAT
  GAAGCTCAACAGCAATTGTATCAGTATGATAGTCAAAATAGGGATAGTATGTATGCATCA
  TTTGATGGCAAGATTATGATCAAGAATATAGATAATGACAGTGATAATCAACCTATACTG
  TATCTCATTTCAAAGGAGTCTCATATTAAAACGTCTGTTACGGAGTTTGATTTAAACAAA
  GTCAAAAAAGGAGACAAAGTTAACTTAAGTATTACGAGTACGGGTAAAACAGGAGCGGGG
  ATTATAAAACAAATCTCTGAGTTGCCTATTAGTTATGAAGAGAATTTAAGTCTTCACGAA
  AATGATGCCCTGCATTTACCAGAAAGTAACAACGATGGGGAATTAGCAAATTCAAAATCG
  ATTTCGGCATCTCCAATATTTAAGAGTAATAACAACTCTGAATTATCAAAGTATGGAGTT
  ATAATTGATGATCTTAGTCTACCAATACGTGCAGGTTATTCGTTGGAAATTAAAGTCCCA
  TTAAATGCGATTAAAATACCTAAATCAGTATTAACAAAAGGCAATAATGTTTTTATTGTT
  AATAAAAATAGTATTGTGGAAAAACGGAATATTCGTATAAAAAGAATCAATGGTGACATT
  ATTGTAGAAAAGGGGTTAAAACCTGGAGACAAGTTAATTAAACAACCTAAAAGTACAATG
  AATGATGGTGATAAGGTTGAGATTTCGTCATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1113
  

⊟Protein

⊟General

• locus tag: SA0191 [new locus tag: SA_RS01135 ]
• symbol: SA0191
• description: hypothetical protein
• length: 370
• theoretical pI: 9.80936
• theoretical MW: 42045.8
• GRAVY: -0.559459

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 53.8)
  and 2 more
  Metabolism Transport and binding proteins Other membrane fusion protein cluster 2 protein (TIGR00999; HMM-score: 26.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 9.8)
• TheSEED  :
  • ABC transporter, RND-adapter-like protein SERP0014
• PFAM:
  TPR (CL0020) Tcf25; Transcriptional repressor TCF25 (PF04910; HMM-score: 13.2)
  Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 13)
  no clan defined Myosin-VI_CBD; Myosin VI cargo binding domain (PF16521; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 4.6
  • Cellwall Score: 2.48
  • Extracellular Score: 2.92
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 33 aa
  • SP(Sec/SPI): 0.635664
  • TAT(Tat/SPI): 0.001514
  • LIPO(Sec/SPII): 0.043977
  • Cleavage Site: CS pos: 33-34. TES-KE. Pr: 0.2258
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15925901 NCBI
• RefSeq: NP_373434 NCBI
• UniProt: A0A0H3JNF0 UniProt

⊟Protein sequence

• MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEKERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDMLINYKINSNKRQQLVDKINDTQHIITEVYLNITKGLNNREIQKKLIEYKQTLYEAQQQLYQYDSQNRDSMYASFDGKIMIKNIDNDSDNQPILYLISKESHIKTSVTEFDLNKVKKGDKVNLSITSTGKTGAGIIKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELSKYGVIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIKRINGDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0191 > SA0192 > SA0193

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications