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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0257 [new locus tag: SA_RS01510 ]
- pan locus tag?: SAUPAN001152000
- symbol: SA0257
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0257 [new locus tag: SA_RS01510 ]
- symbol: SA0257
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 311940..312701
- length: 762
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123036 NCBI
- RefSeq: NP_373503 NCBI
- BioCyc: see SA_RS01510
- MicrobesOnline: 102529 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGTCTAAAGAAGCTGGTCATACATTTTTAGCTAAATTAGGAAAAACTCGTCTACGCCCC
GGTGGTAAAGAAGCAACAGATTGGTTAATACAACAAGGGGCATTTTCACAAGATAAACAA
GTGTTAGAAGTGGCATGTAATATGTGCACAACATCTATTTATCTAGCTCATACATATGGC
TGTCACATTCAAGGCGTTGATATAAATAAGAAAGCATTAGAAAAAGCACAGGAAAATATT
TCAGCAGCAGGTCTTGAATCATATATTCAAGTTCAACAAGCGAATGCTGTTAAATTGCCC
TTTGATGACAATCAATTCGATATCGTTTTAAATGAAGCAATGTTAACAATGTTACCCATC
GCCATAAAGGAAAAAGCATTACGCGAGTACTACCGAGTCTTAAAGCCTGGGGGTATCTTG
TTAACACATGATATTGTCATCGTTAATGAATCACATGCCACACATGTTGTTAAATCATTA
TCTGCAGCAATTAATGTCAATGTCTCACCGCAGACGAAACTTGGCTGGTTAGATTTATAT
CATCAAGCTGGTTTTAATCATGTGCATTATCATACTGGTCCAATGAGTTTAATGACACCA
AAAGGTTTAATTTATGACGAAGGTATTGTTGGAACTATAAAGATTATCAAAAATGCTTTA
AAAAAAGAAAATCGACCAATGTTTTGTAAAATGTTTAAAACGATGACTAAATTGCGTAAA
GATATGAATTATATTACTTTTGTCGCTAAAAAAGAGGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0257 [new locus tag: SA_RS01510 ]
- symbol: SA0257
- description: hypothetical protein
- length: 253
- theoretical pI: 9.42185
- theoretical MW: 28361.9
- GRAVY: -0.18498
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 58.6)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 48.9)and 14 moreBiosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 32.1)Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 31.6)pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 23.9)Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 23.6)Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 22.7)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 21.9)Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 21.6)methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 19)Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 18.5)Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 18.3)Unknown function Enzymes of unknown specificity tRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 16.5)Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 13.4)Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 13.4)Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 12.4)
- TheSEED :
- 2-heptaprenyl-1,4-naphthoquinone methyltransferase (EC 2.1.1.163)
- 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (EC 2.1.1.201)
- PFAM: NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 71.6)Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 70.5)Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 66.6)and 15 moreUbie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 55.2)Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 41.2)Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 39)CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 34.6)UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 30.1)Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 26.4)MTS; Methyltransferase small domain (PF05175; HMM-score: 26)PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 23.1)CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 19.5)Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 18.5)Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 17.9)TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 15.4)TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 13.5)ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 12.8)Methyltransf_10; Protein of unknown function (DUF890) (PF05971; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.077335
- TAT(Tat/SPI): 0.001947
- LIPO(Sec/SPII): 0.0036
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEVACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFDDNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSLSAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIIKNALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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